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Cell No. : Cell Name
RCB1018 : T3M-3  update : 2020/09/24
CommentTrophoblastic cell. Producing chorionic gonadotropin, estrogen, progesterone. Cell growth is slow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name Chorio
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue choriocarcinoma
Classification cancer
Year of origin 1978
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2-3 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 63-71(50) : /64(3),65(8),66(9),67(11),68(10),69(4)/
Images
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lot info
Reference information Reference 3
User's Publication 1


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Reference
5478  Okabe T, Ishizaka S, Fujisawa M.  Human trophoblastic cells metabolize 25-hydroxyvitamin D3 in vitro.  Jpn J Med  1991  30(4):326-9  PubMed ID: 1658420
2021  Uzumaki H, Okabe T, Sasaki N, Hagiwara K, Takaku F, Tobita M, Yasukawa K, Ito S, Umezawa Y  Identification and characterization of receptors for granulocyte colony-stimulating factor on human placenta and trophoblastic cells.  Proc Natl Acad Sci U S A  1989  86:9323-6  PubMed ID: 2480598
2020  Okabe T, Sasaki N, Matsuzaki M, Imai Y, Kaneko Y, Matsuzaki F, Takaku F, Tsushima T  Establishment and characterization of a new human functional cell line from a choriocarcinoma.  Cancer Res  1983  43:4920-6  PubMed ID: 6603906

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User's Publication
10703  Warthan MD, Washington SL, Franzese SE, Ramus RM, Kim KR, York TP, Stratikos E, Strauss JF, Lee ED.  The role of endoplasmic reticulum aminopeptidase 2 in modulating immune detection of choriocarcinoma.  Biol. Reprod.  2018  98:309-322  PubMed ID: 29324974



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