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Cell No. : Cell Name
RCB1015 : T3M-1 Clone2  update : 2021/02/26
CommentG-CSF producing oral squamous cell carcinoma, the same patient as T3M-1 Cl-10 and CJM.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name CJM-25 C5
Original cell T3M-1
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 33 years
Tissue oral cavity
Disease name squamous cell carcinoma
Metastastatic ability Yes
Metastatsd tissue the thoracic cavity
Classification cancer
Year of origin 1997
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/10-14 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 59-130(50) : /61(8),62(13),63(8),64(11)/
Reference information Reference 6
User's Publication 7


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Reference
2029  Okabe T, Kasuga T  Clonal heterogeneity of a human functioning tumor which produces granulocyte colony-stimulating factor.  Jpn J Clin Oncol  1990  20:349-55  PubMed ID: 1704443
1998  Sato K, Fujii Y, Kakiuchi T, Kasono K, Imamura H, Kondo Y, Mano H, Okabe T, Asano S, Takaku F, et al.  Paraneoplastic syndrome of hypercalcemia and leukocytosis caused by squamous carcinoma cells (T3M-1) producing parathyroid hormone-related protein, interleukin 1 alpha, and granulocyte colony-stimulating factor.  Cancer Res  1989  49:4740-6  PubMed ID: 2474371
2018  Sato K, Mimura H, Han, D C, Kakiuchi T, Ueyama Y, Ohkawa H, Okabe T, Kondo Y, Ohsawa N, Tsushima T, et al.  Production of bone-resorbing activity and colony-stimulating activity in vivo and in vitro by a human squamous cell carcinoma associated with hypercalcemia and leukocytosis.  J Clin Invest  1986  78:145-54  PubMed ID: 3487554
2019  Okabe T, Takaku F  Long-term cultivation of a human colony-stimulating factor-producing cell line in a protein-free chemically defined medium.  Cancer Res  1984  44:4503-6  PubMed ID: 6331879
2051  Okabe T, Suzuki A, Ohsawa N, Kosaka K, Terasima T.  Immune elimination of host fibroblasts for the cultivation of human tumors transplanted into nude mice.  Cancer Res  1979  39(10):4189-94  PubMed ID: 476654
2017  Okabe T, Sato N, Kondo Y, Asano S, Ohsawa N, Kosaka K, Ueyama Y  Establishment and characterization of a human cancer cell line that produces human colony-stimulating factor.  Cancer Res  1978  38:3910-7  PubMed ID: 308840

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User's Publication
9935  Suganya S AA, Kochurani KJ, Nair MG, Louis JM, Sankaran S, Rajagopal R, Kumar KS, Abraham P, P G B, Sebastian P, Somananthan T, Maliekal TT.  TM1-IR680 peptide for assessment of surgical margin and lymph node metastasis in murine orthotopic model of oral cancer.  Sci Rep  2016  6:36726  PubMed ID: 27827443
6344  Parvathy M, Sreeja S, Kumar R, Pillai MR.  Potential role of p21 Activated Kinase 1 (PAK1) in the invasion and motility of oral cancer cells.  BMC Cancer.  2016  16 Suppl 1:293  PubMed ID: 27229476
6099  Kishimoto K, Yoshida S, Ibaragi S, Yoshioka N, Okui T, Hu GF, Sasaki A.  Hypoxia-induced up-regulation of angiogenin, besides VEGF, is related to progression of oral cancer.  Oral Oncol  2012  48(11):1120-7  PubMed ID: 22694909
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
6141  Nakamura E, Kozaki K, Tsuda H, Suzuki E, Pimkhaokham A, Yamamoto G, Irie T, Tachikawa T, Amagasa T, Inazawa J, Imoto I.  Frequent silencing of a putative tumor suppressor gene melatonin receptor 1 A (MTNR1A) in oral squamous-cell carcinoma.  Cancer Sci  2008  99(7):1390-400  PubMed ID: 18452558
2906  Nishimaki, Haruaki, Kasai, Kenji, Kozaki, Ken ichi, Takeo, Tomohiro, Ikeda, Hiroshi, Saga, Shinsuke, Nitta, Masakazu, Itoh, Gen  A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line.  Biochem Biophys Res Commun  2004  314:313-20  PubMed ID: 14733907
6156  Nakayama S, Sasaki A, Mese H, Alcalde RE, Tsuji T, Matsumura T.  The E-cadherin gene is silenced by CpG methylation in human oral squamous cell carcinomas.  Int J Cancer.  2001  93(5):667-73  PubMed ID: 11477576



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