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Cell No. : Cell Name
RCB1009 : ECC12  update : 2022/03/28
CommentStomach cancer (small cell cancer + glandular cancer)-derived cell line. Expressing creatinine kinase of brain isoenzyme etc. ( PubMed ID: 8392984). Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Motoyama, Teiichi
Originator Ishihara, Noriko
Year of deposit 1994
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 63 years
Tissue stomach, subcutis
Disease name small-cell gastrointestinal
Metastastatic ability Yes
Metastatsd tissue liver
Genetics small-cell gastrointestinal
Classification cancer
Year of origin 1986
History originator
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1189
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics 100U/ml Penicillin + 100μg/ml Streptomycin
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2-3 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) (-)
Chromosome mode 40-52(50) : /47(4), 48(23), 49(5), 50(8), 51(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 8

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11693  T Fujiwara, T Motoyama, N Ishihara, H Watanabe, T Kumanishi, K Kato, H Ichinose, T Nagatsu  Characterization of four new cell lines derived from small-cell gastrointestinal carcinoma  Int J Cancer  1993  54(6):965-71  PubMed ID: 8392984   DOI: 10.1002/ijc.2910540617

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User's Publication
20739  Chen P, Wang W, Wong SW, Li J, Wu Q, Zhang SD, Lin Y, Kwok HF.  RUNDC3A regulates SNAP25-mediated chemotherapy resistance by binding AKT in gastric neuroendocrine carcinoma (GNEC).  Cell Death Discov  2022  8(1):296  PubMed ID: 35752613   DOI: 10.1038/s41420-022-01084-4
16129  Nagamura Y, Miyazaki M, Nagano Y, Tomiyama A, Ohki R, Yanagihara K, Sakai R, Yamaguchi H.  SHP2 as a Potential Therapeutic Target in Diffuse-Type Gastric Carcinoma Addicted to Receptor Tyrosine Kinase Signaling  Cancers (Basel)  2021  13(17):4309  PubMed ID: 34503119   DOI: 10.3390/cancers13174309
13233  Xie J, Chen P, Xie H, Sun Y, Huang Z, Wei R, Miao Z, Wang Q, Zhang SD, Wong KH, Lin Y, Huang C, Kwok HF.  Exploration of gastric neuroendocrine carcinoma (GNEC) specific signaling pathways involved in chemoresistance via transcriptome and in vitro analysis.  Comput Struct Biotechnol J  2020    PubMed ID: 33033581   DOI: 10.1016/j.csbj.2020.09.016
6405  Matsuo K, Taniguchi K, Hamamoto H, Ito Y, Futaki S, Inomata Y, Shima T, Asakuma M, Lee SW, Tanaka K, Okuda J, Kondo Y, Uchiyama K.  Delta-like 3 localizes to neuroendocrine cells and plays a pivotal role in gastrointestinal neuroendocrine malignancy.  Cancer Sci  2019  110(10):3122-3131  PubMed ID: 31369178   DOI: 10.1111/cas.14157
6413  Lin JX, Weng XF, Xie XS, Lian NZ, Qiu SL, Wang JB, Lu J, Chen QY, Cao LL, Lin M, Tu RH, Yang YH, Liu SJ, Hu M, Lin YK, Huang CM, Zheng CH, Li P, Xie JW.  CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling.  Cancer Cell Int  2019  7;19:282  PubMed ID: 31728130   DOI: 10.1186/s12935-019-0997-5
5302  Yamaguchi H, Takanashi M, Yoshida N, Ito Y, Kamata R, Fukami K, Yanagihara K, Sakai R.  Saracatinib impairs the peritoneal dissemination of diffuse-type gastric carcinoma cells resistant to Met and fibroblast growth factor receptor inhibitors.  Cancer Sci  2014  105(5):528-36  PubMed ID: 24612061   DOI: 10.1111/cas.12387
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
6412  Shida T, Furuya M, Nikaido T, Hasegawa M, Koda K, Oda K, Miyazaki M, Kishimoto T, Nakatani Y, Ishikura H.  Sonic Hedgehog-Gli1 signaling pathway might become an effective therapeutic target in gastrointestinal neuroendocrine carcinomas.  Cancer Biol Ther  2006  5(11):1530-8  PubMed ID: 17102592   DOI: 10.4161/cbt.5.11.3458

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