Cell No. : Cell Name
RCB1009 : ECC12
update : 2025/05/27
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| Comment | Stomach cancer (small cell cancer + glandular cancer)-derived cell line. Expressing creatinine kinase of brain isoenzyme etc. ( PubMed ID: 8392984). Application consideration |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Motoyama, Teiichi
|
| Originator |
Ishihara, Noriko
|
| Year of deposit |
1994
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
63 years
|
| Tissue |
stomach, subcutis
|
| Disease name |
small-cell gastrointestinal
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
liver
|
| Genetics |
small-cell gastrointestinal
|
| Classification |
cancer
|
| Year of origin |
1986
|
| History |
originator
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_1189
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|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
RPMI1640 + 10% FBS
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| Antibiotics |
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100U/ml Penicillin + 100μg/ml Streptomycin
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
|
| SC frequency |
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Subculture : once/2-3 weeks, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
(-)
|
| Chromosome mode |
|
40-52(50) : /47(4), 48(23), 49(5), 50(8), 51(1)/
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| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
8
|
| User's Publication |
|
20739
Chen P, Wang W, Wong SW, Li J, Wu Q, Zhang SD, Lin Y, Kwok HF.
RUNDC3A regulates SNAP25-mediated chemotherapy resistance by binding AKT in gastric neuroendocrine carcinoma (GNEC).
Cell Death Discov
2022
8(1):296
PubMed ID: 35752613
DOI: 10.1038/s41420-022-01084-4
|
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16129
Nagamura Y, Miyazaki M, Nagano Y, Tomiyama A, Ohki R, Yanagihara K, Sakai R, Yamaguchi H.
SHP2 as a Potential Therapeutic Target in Diffuse-Type Gastric Carcinoma Addicted to Receptor Tyrosine Kinase Signaling
Cancers (Basel)
2021
13(17):4309
PubMed ID: 34503119
DOI: 10.3390/cancers13174309
|
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13233
Xie J, Chen P, Xie H, Sun Y, Huang Z, Wei R, Miao Z, Wang Q, Zhang SD, Wong KH, Lin Y, Huang C, Kwok HF.
Exploration of gastric neuroendocrine carcinoma (GNEC) specific signaling pathways involved in chemoresistance via transcriptome and in vitro analysis.
Comput Struct Biotechnol J
2020
PubMed ID: 33033581
DOI: 10.1016/j.csbj.2020.09.016
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6405
Matsuo K, Taniguchi K, Hamamoto H, Ito Y, Futaki S, Inomata Y, Shima T, Asakuma M, Lee SW, Tanaka K, Okuda J, Kondo Y, Uchiyama K.
Delta-like 3 localizes to neuroendocrine cells and plays a pivotal role in gastrointestinal neuroendocrine malignancy.
Cancer Sci
2019
110(10):3122-3131
PubMed ID: 31369178
DOI: 10.1111/cas.14157
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6413
Lin JX, Weng XF, Xie XS, Lian NZ, Qiu SL, Wang JB, Lu J, Chen QY, Cao LL, Lin M, Tu RH, Yang YH, Liu SJ, Hu M, Lin YK, Huang CM, Zheng CH, Li P, Xie JW.
CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling.
Cancer Cell Int
2019
7;19:282
PubMed ID: 31728130
DOI: 10.1186/s12935-019-0997-5
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5302
Yamaguchi H, Takanashi M, Yoshida N, Ito Y, Kamata R, Fukami K, Yanagihara K, Sakai R.
Saracatinib impairs the peritoneal dissemination of diffuse-type gastric carcinoma cells resistant to Met and fibroblast growth factor receptor inhibitors.
Cancer Sci
2014
105(5):528-36
PubMed ID: 24612061
DOI: 10.1111/cas.12387
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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6412
Shida T, Furuya M, Nikaido T, Hasegawa M, Koda K, Oda K, Miyazaki M, Kishimoto T, Nakatani Y, Ishikura H.
Sonic Hedgehog-Gli1 signaling pathway might become an effective therapeutic target in gastrointestinal neuroendocrine carcinomas.
Cancer Biol Ther
2006
5(11):1530-8
PubMed ID: 17102592
DOI: 10.4161/cbt.5.11.3458
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