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Cell No. : Cell Name
RCB1008 : KP4-3  update : 2020/09/24
CommentPancreatic ductal cell carcinoma. PTHrP producing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nishi, Y. & Nawata, H.
Originator Nawata, H. & Nishi, Y.
Year of deposit 1994
Another name KP4-3(Cl-5)
Original cell KP4
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 50 years
Tissue pancreas, ascites meta
Case history pancreatic ductal cell carcino
Metastastatic ability Yes
Metastatsd tissue liver, lung, etc.
Classification cancer
Year of origin 1990
History Direct from originator.
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 61-73(50) : /63(8),64(9),65(9),66(7),67(6),68(2)/
Reference information Reference 1
User's Publication 1


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Reference
1840  Nishi, Y., Haji, M., Takayanagi, R., Iguchi, H., Shimazoe, T., Hirata, J., and Nawata, H.  Establishment and characterization of PTHrP-producing human pancreatic cancer cell line  Int J Oncol  1994  5:33-39  PubMed ID: 21559554

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User's Publication
9183  Maria New, Tim Van Acker Jun-Ichi Sakamaki, Ming Jiang, Rebecca E Saunders, Jaclyn Long, Victoria M-Y Wang, Axel Behrens, Joana Cerveira, Padhmanand Sudhakar, Tamas Korcsmaros, Harold B J Jefferies, Kevin M Ryan, Michael Howell, Sharon A Tooze  MDH1 and MPP7 Regulate Autophagy in Pancreatic Ductal Adenocarcinoma  Cancer Res  2019  79 (8), 1884-1898  PubMed ID: 30765601



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