Cell No. : Cell Name
RCB1007 : KP4-2
update : 2024/11/12
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| Comment | Pancreatic ductal cell carcinoma. PTHrP producing. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishi, Y. & Nawata, H.
|
| Originator |
Nawata, H. & Nishi, Y.
|
| Year of deposit |
1994
|
| Another name |
KP4-2(Cl-1)
|
| Original cell |
KP4
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
50 years
|
| Tissue |
pancreas, ascites meta
|
| Disease name |
pancreatic ductal cell carcino
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
liver, lung, etc.
|
| Classification |
cancer
|
| Year of origin |
1990
|
| History |
Direct from originator.
|
| Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_8728
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM/HamF12 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
60-67(50) : 60(7),61(5),62(12),63(10),64(7),65(5)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
|
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