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Cell No. : Cell Name
RCB1006 : KP4-1  update : 2022/03/28
CommentPancreatic ductal cell carcinoma. PTHrP producing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishi, Y. & Nawata, H.
Originator Nawata, H. & Nishi, Y.
Year of deposit 1994
Another name KP4-1(Cl-4)
Original cell KP4
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 50 years
Tissue pancreas, ascites meta
Disease name pancreatic ductal cell carcino
Metastastatic ability Yes
Metastatsd tissue liver, lung, etc.
Classification cancer
Year of origin 1990
History Direct from originator.
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8727
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 65-71(50) : 65(3),66(12),67(10),68(9),70(5),71(2)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 9

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1840  Nishi, Y., Haji, M., Takayanagi, R., Iguchi, H., Shimazoe, T., Hirata, J., and Nawata, H.  Establishment and characterization of PTHrP-producing human pancreatic cancer cell line  Int J Oncol  1994  5:33-39  PubMed ID: 21559554  

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User's Publication
20773  Han JH, Kim YK, Kim H, Lee J, Oh MJ, Kim SB, Kim M, Kim KH, Yoon HJ, Lee MS, Minna JD, White MA, Kim HS.  Snail acetylation by autophagy-derived acetyl-coenzyme A promotes invasion and metastasis of KRAS-LKB1 co-mutated lung cancer cells.  Cancer Commun (Lond)  2022    PubMed ID: 35838183   DOI: 10.1002/cac2.12332
20431  Shiozaki A, Konishi T, Kosuga T, Kudou M, Kurashima K, Inoue H, Shoda K, Arita T, Konishi H, Morimura R, Komatsu S, Ikoma H, Toma A, Kubota T, Fujiwara H, Okamoto K, Otsuji E.  Roles of voltage‑gated potassium channels in the maintenance of pancreatic cancer stem cells.  Int J Oncol  2021  59(4)  PubMed ID: 34414448   DOI: 10.3892/ijo.2021.5256
14726  Imamura T, Komatsu S, Ichikawa D, Miyamae M, Okajima W, Ohashi T, Kiuchi J, Nishibeppu K, Konishi H, Shiozaki A, Morimura R, Ikoma H, Ochiai T, Okamoto K, Taniguchi H, Otsuji E.  Depleted tumor suppressor miR-107 in plasma relates to tumor progression and is a novel therapeutic target in pancreatic cancer  Sci Rep  2017  7(1):5708  PubMed ID: 28720759   DOI: 10.1038/s41598-017-06137-8
9592  Hijiya N, Tsukamoto Y, Nakada C, Tung Nguyen L, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.  Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma.  Cancer Res.  2016  76:2612-25  PubMed ID: 26941286   DOI: 10.1158/0008-5472.CAN-15-1846
7168  Miyamae M, Komatsu S, Ichikawa D, Kawaguchi T, Hirajima S, Okajima W, Ohashi T, Imamura T, Konishi H, Shiozaki A, Morimura R, Ikoma H, Ochiai T, Okamoto K, Taniguchi H, Otsuji E.  Plasma microRNA profiles: identification of miR-744 as a novel diagnostic and prognostic biomarker in pancreatic cancer.  Br. J. Cancer  2015  113:1467-76  PubMed ID: 26505678   DOI: 10.1038/bjc.2015.366
5024  Kawaguchi T, Komatsu S, Ichikawa D, Morimura R, Tsujiura M, Konishi H, Takeshita H, Nagata H, Arita T, Hirajima S, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E.  Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer.  Br J Cancer  2013  108(2):361-9  PubMed ID: 23329235   DOI: 10.1038/bjc.2012.546
5032  Igarashi S, Matsubara T, Harada K, Ikeda H, Sato Y, Sasaki M, Matsui O, Nakanuma Y.  Bile duct expression of pancreatic and duodenal homeobox 1 in perihilar cholangiocarcinogenesis.  Histopathology  2012  61(2):266-76  PubMed ID: 22594685   DOI: 10.1111/j.1365-2559.2012.04218.x
7643  Morimura R, Komatsu S, Ichikawa D, Takeshita H, Tsujiura M, Nagata H, Konishi H, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E.  Novel diagnostic value of circulating miR-18a in plasma of patients with pancreatic cancer.  Br. J. Cancer  2011  105:1733-40  PubMed ID: 22045190   DOI: 10.1038/bjc.2011.453
3717  Wada M, Yazumi S, Takaishi S, Hasegawa K, Sawada M, Tanaka H, Ida H, Sakakura C, Ito K, Ito Y, Chiba T.  Frequent loss of RUNX3 gene expression in human bile duct and pancreatic cancer cell lines.  Oncogene  2004  23(13):2401-7  PubMed ID: 14743205   DOI: 10.1038/sj.onc.1207395

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