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Cell No. : Cell Name
RCB0998 : 117-13 update : 2024/01/17 |
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| Comment | Anti-lactotetraosylceramide antibody producing. Discriminates metastatic cells from mesothelial cell | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Tsuchida, Kazuo | ||
| Originator | Tsuchida, Kazuo | |||
| Year of deposit | 1994 | |||
| Another name | anti-OMC-4 117-13 | |||
| Original cell | anti-OMC-4 117 | |||
| Animal | _mouse x mouse < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | |||
| Year of origin | 1988 | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_T730 | hybridoma | Fusion_cell_1 name | spleen |
| Fusion_cell_1 strain | mouse, BALB/c | |||
| Fusion_cell_2 name | SP2/0-Ag14 | |||
| Fusion_cell_2 strain | mouse, BALB/c | Fusion method | PEG | Antibody name | AD117m |
| Subclass | IgM | |||
| Immunogen | OMC4 cells | Antigen_name | OMC4 cells | |
| Antigen_epitope | lactotetraosylceramide | Antigen_positive | uterine adenocarcinoma | |
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | DMEM (low glucose) + 10% FBS | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Antibody subclass | IgM | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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| Reference |
| 2016 Tsuchida, K., Okamoto, Y., Yamada, T., Ueda, M., Iijima, J., Shiina, Y., Hirano, H., Rokukawa, C., Kasama, T., Taki, T., Handa, S., Ueki, M., Adachi, K. Discrimination of Metastatic Cells from Mesothelial Cells in Effusion Cytology by Monoclonal Antibody AD117m Directed Against Lactotetraosylceramide Acta Histochem. Cytochem. 1993 26:515-530 |
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| User's Publication |
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