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Cell No. : Cell Name
RCB0998 : 117-13  update : 2022/03/25
CommentAnti-lactotetraosylceramide antibody producing. Discriminates metastatic cells from mesothelial cell
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tsuchida, Kazuo
Originator Tsuchida, Kazuo
Year of deposit 1994
Another name anti-OMC-4 117-13
Original cell anti-OMC-4 117
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Year of origin 1988
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_T730
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name SP2/0-Ag14
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG
Antibody name AD117m
Subclass IgM
Immunogen OMC4 cells
Antigen_name OMC4 cells
Antigen_epitope lactotetraosylceramide
Antigen_positive uterine adenocarcinoma
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
Reference information Reference 1
User's Publication 0


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Reference
2016  Tsuchida, K., Okamoto, Y., Yamada, T., Ueda, M., Iijima, J., Shiina, Y., Hirano, H., Rokukawa, C., Kasama, T., Taki, T., Handa, S., Ueki, M., Adachi, K.  Discrimination of Metastatic Cells from Mesothelial Cells in Effusion Cytology by Monoclonal Antibody AD117m Directed Against Lactotetraosylceramide  Acta Histochem. Cytochem.  1993  26:515-530   

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