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Cell No. : Cell Name
RCB0996 : Mv.1.Lu(NBL-7)  update : 2019/12/11
CommentAleutian mink cells. Used for focus forming assays for murine and ferine sarcoma viruses. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88050503 prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88050503 prior to order.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1994
Animal mink < Mammals
Age at sampling embryo
Tissue embryo/fetus, lung
Classification transformed
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Reference information Reference 1
User's Publication 3


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Reference
1830  Henderson, I C, Lieber, M M, Todaro, G J  Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses.  Virology  1974  60:282-7  PubMed ID: 4366800

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User's Publication
9079  Manabu Shimojoh, Taku Kojima, Kohji Nakajima, Kiyoshige Hatta, Akira Katoh, Keisuke Kurita  Branched Chitins as Nonnatural Immunomodulatory Biopolymers: Secretions of TNF-alpha and NO by Direct Stimulation of Macrophages  Biomacromolecules  2010  11 (5), 1212-6  PubMed ID: 20415471
8776  Hayata D, Fukuta K, Matsumoto K, Adachi E, Hanada K, Adachi K, Nakamura T.  Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I.  J. Biotechnol.  2008  133:478-85  PubMed ID: 18178280
3590  Koito A, Kameyama Y, Cheng-Mayer C, Matsushita S.  Susceptibility of mink (Mustera vision)-derived cells to replication by human immunodeficiency virus type 1.  J Virol  2003  77(9):5109-17  PubMed ID: 12692213



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