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Cell No. : Cell Name
RCB0996 : Mv.1.Lu(NBL-7)  update : 2023/12/25
CommentAleutian mink cells. Used for focus forming assays for murine and ferine sarcoma viruses. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 88050503 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88050503 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Year of deposit 1994
Animal _mink < Mammals
Age at sampling embryo
Tissue embryo/fetus, lung
Classification transformed
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0593
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Reference information Reference 1
User's Publication 7

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1830  Henderson, I C, Lieber, M M, Todaro, G J  Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses.  Virology  1974  60:282-7  PubMed ID: 4366800   DOI: 10.1016/0042-6822(74)90386-9

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User's Publication
16064  Tahira Y, Sakai K, Sato H, Imamura R, Matsumoto K.  Dimer Interface in Natural Variant NK1 Is Dispensable for HGF-Dependent Met Receptor Activation  Int J Mol Sci  2021  22(17):9240  PubMed ID: 34502141   DOI: 10.3390/ijms22179240
15445  Erika Hayashi, Kaori Hachiya, Satoshi Kojo, Muhammad Baghdadi, Shintaro Takeuchi, Hiroyuki Yamanaka, Hirotak Abe, Haruka Wada, Ken-ichiro Seino  α-MSH stimulation contributes to TGF-β1 production via MC1R-MITF signaling pathway in melanoma cell  Inflammation and Regeneration  2015  35:244-254    DOI: 10.2492/inflammregen.35.244
9079  Manabu Shimojoh, Taku Kojima, Kohji Nakajima, Kiyoshige Hatta, Akira Katoh, Keisuke Kurita  Branched Chitins as Nonnatural Immunomodulatory Biopolymers: Secretions of TNF-alpha and NO by Direct Stimulation of Macrophages  Biomacromolecules  2010  11 (5), 1212-6  PubMed ID: 20415471   DOI: 10.1021/bm9013793
8776  Hayata D, Fukuta K, Matsumoto K, Adachi E, Hanada K, Adachi K, Nakamura T.  Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I.  J. Biotechnol.  2008  133:478-85  PubMed ID: 18178280   DOI: 10.1016/j.jbiotec.2007.11.006
18847  Yamamoto Y, Ueda Y, Itoh T, Iwamoto A, Yamagishi H, Shimagaki M, Teramoto K.  A novel immunotherapeutic modality with direct hemoperfusion targeting transforming growth factor-beta prolongs the survival of tumor-bearing rats  Oncol Rep  2006  16(6):1277-84  PubMed ID: 17089050  
3590  Koito A, Kameyama Y, Cheng-Mayer C, Matsushita S.  Susceptibility of mink (Mustera vision)-derived cells to replication by human immunodeficiency virus type 1.  J Virol  2003  77(9):5109-17  PubMed ID: 12692213   DOI: 10.1128/jvi.77.9.5109-5117.2003
11032  K Kitamura, S i Aota, R Sakamoto, S I Yoshikawa, K Okazaki  Smad7 selectively interferes with different pathways of activin signaling and inhibits erythroid leukemia cell differentiation  Blood  2000  95(11):3371-9  PubMed ID: 10828018  

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