Cell No. : Cell Name RCB0996 : Mv.1.Lu(NBL-7)  update : 2025/03/21
Comment			Aleutian mink cells. Used for focus forming assays for murine and ferine sarcoma viruses. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 88050503 prior to order.
Comment from the depositor
Terms and conditions			1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88050503 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	ECACC
		Year of deposit	1994
		Animal	_mink < Mammals
		Age at sampling	embryo
		Tissue	embryo/fetus, lung
		Classification	transformed
		History	overseas, ECACC
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_0593
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS + 0.1mM NEAA
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8-10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		DNA barcoding		OK
Reference information		Reference	1
		User's Publication	7

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Reference

1830  Henderson, I C, Lieber, M M, Todaro, G J  Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses.  Virology  1974  60:282-7  PubMed ID: 4366800   DOI: 10.1016/0042-6822(74)90386-9

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User's Publication

16064  Tahira Y, Sakai K, Sato H, Imamura R, Matsumoto K.  Dimer Interface in Natural Variant NK1 Is Dispensable for HGF-Dependent Met Receptor Activation  Int J Mol Sci  2021  22(17):9240  PubMed ID: 34502141   DOI: 10.3390/ijms22179240

15445  Erika Hayashi, Kaori Hachiya, Satoshi Kojo, Muhammad Baghdadi, Shintaro Takeuchi, Hiroyuki Yamanaka, Hirotak Abe, Haruka Wada, Ken-ichiro Seino  α-MSH stimulation contributes to TGF-β1 production via MC1R-MITF signaling pathway in melanoma cell  Inflammation and Regeneration  2015  35:244-254    DOI: 10.2492/inflammregen.35.244

9079  Manabu Shimojoh, Taku Kojima, Kohji Nakajima, Kiyoshige Hatta, Akira Katoh, Keisuke Kurita  Branched Chitins as Nonnatural Immunomodulatory Biopolymers: Secretions of TNF-alpha and NO by Direct Stimulation of Macrophages  Biomacromolecules  2010  11 (5), 1212-6  PubMed ID: 20415471   DOI: 10.1021/bm9013793

8776  Hayata D, Fukuta K, Matsumoto K, Adachi E, Hanada K, Adachi K, Nakamura T.  Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I.  J. Biotechnol.  2008  133:478-85  PubMed ID: 18178280   DOI: 10.1016/j.jbiotec.2007.11.006

18847  Yamamoto Y, Ueda Y, Itoh T, Iwamoto A, Yamagishi H, Shimagaki M, Teramoto K.  A novel immunotherapeutic modality with direct hemoperfusion targeting transforming growth factor-beta prolongs the survival of tumor-bearing rats  Oncol Rep  2006  16(6):1277-84  PubMed ID: 17089050

3590  Koito A, Kameyama Y, Cheng-Mayer C, Matsushita S.  Susceptibility of mink (Mustera vision)-derived cells to replication by human immunodeficiency virus type 1.  J Virol  2003  77(9):5109-17  PubMed ID: 12692213   DOI: 10.1128/jvi.77.9.5109-5117.2003

11032  K Kitamura, S i Aota, R Sakamoto, S I Yoshikawa, K Okazaki  Smad7 selectively interferes with different pathways of activin signaling and inhibits erythroid leukemia cell differentiation  Blood  2000  95(11):3371-9  PubMed ID: 10828018