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Cell No. : Cell Name
RCB0995 : MDCK  update : 2022/09/22
CommentCanine kidney epithelial. Sensitive to wide range of viruses. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA84121903 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA84121903 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Originator Madin, S. H. & Darby, N. B.
Year of deposit 1994
Animal dog < Mammals
Genus Canis
Species familiaris
Tissue kidney
Classification other
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0422
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 112

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2581  Oberleithner H, Vogel U, Kersting U, Steigner W  Madin-Darby canine kidney cells. II. Aldosterone stimulates Na+/H+ and Cl-/HCO3- exchange.  Pflugers Arch  1990  416:533-9  PubMed ID: 2172916   DOI: 10.1007/bf00382686
260  Gaush CR, Hard WL, Smith TF.  Characterization of an established line of canine kidney cells (MDCK)  Proc Soc Exp Biol Med.  1966  122(3):931-5  PubMed ID: 5918973  

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User's Publication
15686  Hosokawa-Muto J, Sassa-O'Brien Y, Fujinami Y, Nakahara H.  Analysis Comparison for Rapid Identification of Pathogenic Virus from Infected Tissue Samples  Diagnostics (Basel)  2022  12(1):196  PubMed ID: 35054363   DOI: 10.3390/diagnostics12010196
16141  Hatano H, Meng F, Sakata M, Matsumoto A, Ishihara K, Miyahara Y, Goda T.  Transepithelial delivery of insulin conjugated with phospholipid-mimicking polymers via biomembrane fusion-mediated transcellular pathways  Acta Biomater  2022  140:674-685  PubMed ID: 34896268   DOI: 10.1016/j.actbio.2021.12.003
16436  Hirose R, Miyazaki H, Bandou R, Watanabe N, Yoshida T, Daidoji T, Itoh Y, Nakaya T.  Stability of SARS-CoV-2 and influenza virus varies across different paper types  J Infect Chemother  2022  28(2):252-256  PubMed ID: 34799238   DOI: 10.1016/j.jiac.2021.11.006
16738  Soshi Seike, Masaya Takehara, Keiko Kobayashi, Masahiro Nagahama  Critical Residues of Clostridium perfringens Delta-Toxin for Oligomerization and Receptor Binding  BPB Reports  2022  5:66-69    DOI: 10.1248/bpbreports.5.3_66
20866  Matsuura R, Kawamura A, Matsumoto Y, Iida Y, Kanayama M, Kurokawa M, Aida Y.  Epigallocatechin Gallate Stabilized by Cyclodextrin Inactivates Influenza Virus and Human Coronavirus 229E.  Microorganisms  2022  10(9)  PubMed ID: 36144398   DOI: 10.3390/microorganisms10091796
20146  Chang AC, Uto K, Homma K, Nakanishi J.  Viscoelastically tunable substrates elucidate the interface-relaxation-dependent adhesion and assembly behaviors of epithelial cells.  Biomaterials  2021  274:120861  PubMed ID: 33991949   DOI: 10.1016/j.biomaterials.2021.120861
14235  Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, Murakawa Y, Takaori-Kondo A, Terai K, Matsuda M.  Intravital imaging identifies the VEGF-TXA2 axis as a critical promoter of PGE2 secretion from tumor cells and immune evasion  Cancer Res  2021  canres.4245.2020  PubMed ID: 34035084   DOI: 10.1158/0008-5472.CAN-20-4245
20156  Oyama TG, Oyama K, Kimura A, Yoshida F, Ishida R, Yamazaki M, Miyoshi H, Taguchi M.  Collagen hydrogels with controllable combined cues of elasticity and topography to regulate cellular processes.  Biomed Mater  2021    PubMed ID: 34030146   DOI: 10.1088/1748-605X/ac0452
15520  Tomoko Gowa Oyama, Kotaro Oyama, Hiromi Miyoshi, Mitsumasa Taguchi  3D cell sheets formed via cell-driven buckling-delamination of patterned thin films  Materials & Design  2021  208:109975    DOI: 10.1016/j.matdes.2021.109975
20409  Nagahama M, Kobayashi K, Takehara M.  Cathepsin Release from Lysosomes Promotes Endocytosis of Clostridium perfringens Iota-Toxin.  Toxins (Basel)  2021  13(10)  PubMed ID: 34679014   DOI: 10.3390/toxins13100721
20456  Hirose R, Itoh Y, Ikegaya H, Miyazaki H, Watanabe N, Yoshida T, Bandou R, Daidoji T, Nakaya T.  Evaluation of the Residual Disinfection Effects of Commonly Used Skin Disinfectants against Viruses: An Innovative Contact Transmission Control Method.  Environ Sci Technol  2021  55(23):16044-16055  PubMed ID: 34841856   DOI: 10.1021/acs.est.1c05296
16046  Yamamoto K, Miura H, Ishida M, Mii Y, Kinoshita N, Takada S, Ueno N, Sawai S, Kondo Y, Aoki K.  Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis  Nat Commun  2021  12(1):7145  PubMed ID: 34880255   DOI: 10.1038/s41467-021-27458-3
11826  Matsuo H, Nakanishi J, Noguchi Y, Kitagawa K, Shigemura K, Sunazuka T, Takahashi Y, Ōmura S, Nakashima T.  Nanaomycin K, a new epithelial-mesenchymal transition inhibitor produced by the actinomycete "Streptomyces rosa subsp. notoensis" OS-3966.  J. Biosci. Bioeng.  2020  129(3):291-295  PubMed ID: 31582334   DOI: 10.1016/j.jbiosc.2019.09.007
10499  Naoya Hino, Leone Rossetti, Ariadna Marín-Llauradó, Kazuhiro Aoki, Xavier Trepat, Michiyuki Matsuda, Tsuyoshi Hirashima  ERK-Mediated Mechanochemical Waves Direct Collective Cell Polarization  Dev Cell  2020  S1534-5807(20)30400-7  PubMed ID: 32497487   DOI: 10.1016/j.devcel.2020.05.011
11999  Daidoji T, Kajikawa J, Arai Y, Watanabe Y, Hirose R, Nakaya T.  Infection of Human Tracheal Epithelial Cells by H5 Avian Influenza Virus Is Regulated by the Acid Stability of Hemagglutinin and the pH of Target Cell Endosomes.  Viruses  2020    PubMed ID: 31936692   DOI: 10.3390/v12010082
12119  Suzuki S, Shichinohe S, Itoh Y, Nakayama M, Ishigaki H, Mori Y, Ogata-Nakahara A, Nguyen CT, Okamatsu M, Sakoda Y, Kida H, Ogasawara K.  Low replicative fitness of neuraminidase inhibitor-resistant H7N9 avian influenza a virus with R292K substitution in neuraminidase in cynomolgus macaques compared with I222T substitution.  Antiviral Res.  2020    PubMed ID: 32272175   DOI: 10.1016/j.antiviral.2020.104790
12486  Hirose R, Ikegaya H, Naito Y, Watanabe N, Yoshida T, Bandou R, Daidoji T, Itoh Y, Nakaya T.  Survival of SARS-CoV-2 and influenza virus on the human skin: Importance of hand hygiene in COVID-19  Clin Infect Dis  2020  ciaa1517  PubMed ID: 33009907   DOI: 10.1093/cid/ciaa1517
13015  Nagahama M, Seike S, Ochi S, Kobayashi K, Takehara M.  Clostridium perfringens Epsilon-Toxin Impairs the Barrier Function in MDCK Cell Monolayers in a Ca2+-Dependent Manner.  Toxins (Basel)  2020    PubMed ID: 32365779   DOI: 10.3390/toxins12050286
13033  Ito H, Nishimura H, Kisu T, Hagiwara H, Watanabe O, Kadji FMN, Sato K, Omiya S, Takashita E, Nobusawa E.  Low response in eliciting neuraminidase inhibition activity of sera among recipients of a split, monovalent pandemic influenza vaccine during the 2009 pandemic.  PLoS One  2020    PubMed ID: 32401814   DOI: 10.1371/journal.pone.0233001
13084  Monden Y, Tsukamoto A, Ushida T, Kobayashi E, Nakagawa K, Sakuma I.  An in vitro model of temporal enhancement of epithelium barrier permeability by low-energy shock waves without contrast agents.  Med Biol Eng Comput  2020    PubMed ID: 32591989   DOI: 10.1007/s11517-020-02207-8
13398  Kajiwara N, Nomura N, Ukaji M, Yamamoto N, Kohara M, Yasui F, Sakoda Y, Kida H, Shibasaki F.  Cell-penetrating peptide-mediated cell entry of H5N1 highly pathogenic avian influenza virus.  Sci Rep  2020    PubMed ID: 33093460   DOI: 10.1038/s41598-020-74604-w
13560  Pezzotti G, Zhu W, Adachi T, Horiguchi S, Marin E, Boschetto F, Ogitani E, Mazda O.  Metabolic machinery encrypted in the Raman spectrum of influenza A virus-inoculated mammalian cells  J Cell Physiol  2020  235(6):5146-5170  PubMed ID: 31710091   DOI: 10.1002/jcp.29392
14807  Ishida-Ishihara S, Akiyama M, Furusawa K, Naguro I, Ryuno H, Sushida T, Ishihara S, Haga H.  Osmotic gradients induce stable dome morphogenesis on extracellular matrix  J Cell Sci   2020  133(14):jcs243865  PubMed ID: 32576662   DOI: 10.1242/jcs.243865
16815  Watabe T, Terai K, Sumiyama K, Matsuda M.  Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools  ACS Sens  2020  5(3):719-730  PubMed ID: 32101394   DOI: 10.1021/acssensors.9b01941
11278  Nakanishi J, Sugiyama K, Matsuo H, Takahashi Y, Omura S, Nakashima T.  An Application of Photoactivatable Substrate for the Evaluation of Epithelial-mesenchymal Transition Inhibitors.  Anal Sci  2019    PubMed ID: 30393243   DOI: 10.2116/analsci.18SDP07
11315  Arai Y, Ibrahim MS, Elgendy EM, Daidoji T, Ono T, Suzuki Y, Nakaya T, Matsumoto K, Watanabe Y.  Genetic Compatibility of Reassortants between Avian H5N1 and H9N2 Influenza Viruses with Higher Pathogenicity in Mammals.  J. Virol.  2019    PubMed ID: 30463961   DOI: 10.1128/JVI.01969-18
11362  Hatano H, Goda T, Matsumoto A, Miyahara Y.  Induced Proton Perturbation for Sensitive and Selective Detection of Tight Junction Breakdown.  Anal. Chem.  2019    PubMed ID: 30596415   DOI: 10.1021/acs.analchem.8b05237
5331  Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata S, Matsuda M.  FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics.  Nat Methods  2019  16(10):1029-1036  PubMed ID: 31501546   DOI: 10.1038/s41592-019-0541-5
5344  Tomoko Gowa Oyama, View ORCID ProfileKotaro Oyama, Atsushi Kimura, Fumiya Yoshida, Ryo Ishida, Masashi Yamazaki, Hiromi Miyoshi, View ORCID ProfileMitsumasa Taguchi  Elasticity and Topography-Controlled Collagen Hydrogels Mimicking Native Cellular Milieus  bioRxiv  2019  July 18    DOI: 10.1101/706952
5436  Hirose R, Nakaya T, Naito Y, Daidoji T, Bandou R, Inoue K, Dohi O, Yoshida N, Konishi H, Itoh Y.  Situations Leading to Reduced Effectiveness of Current Hand Hygiene against Infectious Mucus from Influenza Virus-Infected Patients.  mSphere  2019  4(5)  PubMed ID: 31533996   DOI: 10.1128/mSphere.00474-19
11621  Arai Y, Kawashita N, Ibrahim MS, Elgendy EM, Daidoji T, Ono T, Takagi T, Nakaya T, Matsumoto K, Watanabe Y.  PB2 mutations arising during H9N2 influenza evolution in the Middle East confer enhanced replication and growth in mammals.  PLoS Pathog.  2019    PubMed ID: 31265471   DOI: 10.1371/journal.ppat.1007919
11918  Hatano H, Goda T, Matsumoto A, Miyahara Y.  Induced Proton Dynamics on Semiconductor Surfaces for Sensing Tight Junction Formation Enhanced by an Extracellular Matrix and Drug.  ACS Sens  2019    PubMed ID: 31763825   DOI: 10.1021/acssensors.9b01635
12518  Fujii Y, Ochi Y, Tuchiya M, Kajita M, Fujita Y, Ishimoto Y, Okajima T.  Spontaneous Spatial Correlation of Elastic Modulus in Jammed Epithelial Monolayers Observed by AFM  Biophys J  2019  116(6):1152-1158  PubMed ID: 30826009   DOI: 10.1016/j.bpj.2019.01.037
12978  Kojima C, Narita Y, Nakajima Y, Morimoto N, Yoshikawa T, Takahashi N, Handa A, Waku T, Tanaka N.  Modulation of Cell Adhesion and Differentiation on Collagen Gels by the Addition of the Ovalbumin Secretory Signal Peptide.  ACS Biomater Sci Eng  2019    PubMed ID: 33405701   DOI: 10.1021/acsbiomaterials.8b01505
10717  Zhou Y, Saito M, Miyamoto T, Novak P, Shevchuk AI, Korchev YE, Fukuma T, Takahashi Y.  Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy.  Anal. Chem.  2018  90:2891-2895  PubMed ID: 29345134   DOI: 10.1021/acs.analchem.7b05112
10801  Abdellatef SA, Nakanishi J.  Photoactivatable substrates for systematic study of the impact of an extracellular matrix ligand on appearance of leader cells in collective cell migration.  Biomaterials  2018  169:72-84  PubMed ID: 29655082   DOI: 10.1016/j.biomaterials.2018.03.045
11055  Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K.  Automated Adherent Cell Elimination by a High-Speed Laser Mediated by a LightResponsive Polymer  Commun Biol  2018  1:218  PubMed ID: 30534610   DOI: 10.1038/s42003-018-0222-4
11225  Miyamoto R, Kurita S, Tani H, Ikeda T, Ishizaka M, Saima H, Kobayashi M, Tamura K, Bonkobara M.  Canine squamous cell carcinoma cell lines with high expression of survivin are sensitive to survivin inhibitor YM155.  Vet. J.  2018    PubMed ID: 30268330   DOI: 10.1016/j.tvjl.2018.09.001
11494  Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N.  Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport.  J. Cell Biol.  2018  217(12):4164-4183  PubMed ID: 30297389   DOI: 10.1083/jcb.201711178
11238  Nagahama M, Takehara M, Kobayashi K.  Interaction of Clostridium perfringens Iota Toxin and Lipolysis-Stimulated Lipoprotein Receptor (LSR).  Toxins (Basel)  2018    PubMed ID: 30297616   DOI: 10.3390/toxins10100405
4121  Kumeta M, Panina Y, Yamazaki H, Takeyasu K, Yoshimura SH.  N-terminal dual lipidation-coupled molecular targeting into the primary cilium.  Genes Cells  2018    PubMed ID: 29900630   DOI: 10.1111/gtc.12603
4289  Nagahama M, Takehara M, Miyamoto K, Ishidoh K, Kobayashi K.  Acid Sphingomyelinase Promotes Cellular Internalization of Clostridium perfringens Iota-Toxin.  Toxins (Basel)  2018    PubMed ID: 29783772   DOI: 10.3390/toxins10050209
4290  Imanishi A, Murata T, Sato M, Hotta K, Imayoshi I, Matsuda M, Terai K.  A Novel Morphological Marker for the Analysis of Molecular Activities at the Single-cell Level.  Cell Struct. Funct.  2018    PubMed ID: 29962383   DOI: 10.1247/csf.18013
4465  Watanabe Y, Arai Y, Kawashita N, Ibrahim MS, Elgendy EM, Daidoji T, Kajikawa J, Hiramatsu H, Sriwilaijaroen N, Ono T, Takagi T, Takahashi K, Shioda T, Matsumoto K, Suzuki Y, Nakaya T.  Characterization of H5N1 Influenza Virus Quasispecies with Adaptive Hemagglutinin Mutations from Single-Virus Infections of Human Airway Cells.  J. Virol.  2018  92(11)  PubMed ID: 29563293   DOI: 10.1128/JVI.02004-17
5776  Sumaru K, Takagi T, Morishita K, Kanamori T.  Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition.  Biomacromolecules  2018  19(7):2913-2922  PubMed ID: 29641886   DOI: 10.1021/acs.biomac.8b00470
10515  Hirashima T, Hoshuyama M, Adachi T.  In vitro tubulogenesis of Madin-Darby canine kidney (MDCK) spheroids occurs depending on constituent cell number and scaffold gel concentration.  J. Theor. Biol.  2017  435:110-115  PubMed ID: 28923372   DOI: 10.1016/j.jtbi.2017.09.009
10604  Aoki K, Kondo Y, Naoki H, Hiratsuka T, Itoh RE, Matsuda M.  Propagating Wave of ERK Activation Orients Collective Cell Migration.  Dev. Cell  2017  43:305-317.e5  PubMed ID: 29112851   DOI: 10.1016/j.devcel.2017.10.016
11030  Xu X, Yoshizaki H, Ishigaki Y, Kubo E, Minato H, Kiyokawa E.  Upregulation of multiple signaling pathways by Dock5 deletion in epithelial cells  Mol Vis  2017  23:1081-1092  PubMed ID: 29872253  
9124  Masahiro Nagahama, Masaya Takehara, Teruhisa Takagishi, Soshi Seike, Kazuaki Miyamoto, Keiko Kobayashi  Cellular Uptake of Clostridium Botulinum C2 Toxin Requires Acid Sphingomyelinase Activity  Infect Immun  2017  85 (4)   PubMed ID: 28138018   DOI: 10.1128/IAI.00966-16
14258  Hirose R, Nakaya T, Naito Y, Daidoji T, Watanabe Y, Yasuda H, Konishi H, Itoh Y.  Viscosity is an important factor of resistance to alcohol-based disinfectants by pathogens present in mucus  Sci Rep  2017  7(1):13186  PubMed ID: 29030631   DOI: 10.1038/s41598-017-13732-2
14767  Elgendy EM, Arai Y, Kawashita N, Daidoji T, Takagi T, Ibrahim MS, Nakaya T, Watanabe Y.  Identification of polymerase gene mutations that affect viral replication in H5N1 influenza viruses isolated from pigeons  J Gen Virol   2017  98(1):6-17  PubMed ID: 27926816   DOI: 10.1099/jgv.0.000674
7058  Ueyama T, Ninoyu Y, Nishio SY, Miyoshi T, Torii H, Nishimura K, Sugahara K, Sakata H, Thumkeo D, Sakaguchi H, Watanabe N, Usami SI, Saito N, Kitajiri SI.  Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss.  EMBO Mol Med  2016  8:1310-1324  PubMed ID: 27707755   DOI: 10.15252/emmm.201606609
7126  Fukao M, Kondo E, Nishino H, Hattori R, Horie A, Hashimoto Y.  Presence of an H(+)/Quinidine Antiport System in Madin-Darby Canine Kidney Cells.  Eur J Drug Metab Pharmacokinet  2016  41:819-824  PubMed ID: 26628429   DOI: 10.1007/s13318-015-0314-1
9986  Kawabata N, Matsuda M.  Cell Density-Dependent Increase in Tyrosine-Monophosphorylated ERK2 in MDCK Cells Expressing Active Ras or Raf.  PLoS ONE  2016  11:e0167940  PubMed ID: 27936234   DOI: 10.1371/journal.pone.0167940
10863  Kazuya Furusawa, Takeomi Mizutani, Naoki Sasaki  Development of the evaluation system for barrier functions of engineered epithelial lumens  Regen Ther  2016  3:82-89  PubMed ID: 31245477   DOI: 10.1016/j.reth.2016.02.010
5336  Yoshizaki H, Ogiso H, Okazaki T, Kiyokawa E.  Comparative lipid analysis in the normal and cancerous organoids of MDCK cells.  J Biochem  2016  159(6):573-84  PubMed ID: 26783265   DOI: 10.1093/jb/mvw001
5339  Marlar S, Abdellatef SA, Nakanishi J.  Reduced adhesive ligand density in engineered extracellular matrices induces an epithelial-mesenchymal-like transition.  Acta Biomater  2016  39:106-113  PubMed ID: 27163400   DOI: 10.1016/j.actbio.2016.05.006
9117  Soshi Seike, Kazuaki Miyamoto, Keiko Kobayashi, Masaya Takehara, Masahiro Nagahama  Clostridium Perfringens Delta-Toxin Induces Rapid Cell Necrosis  PLoS One  2016  11 (1), e0147957  PubMed ID: 26807591   DOI: 10.1371/journal.pone.0147957
10144  Teruhisa Takagishi, Masataka Oda, Masaya Takehara, Keiko Kobayashi, Masahiro Nagahama  Oligomer Formation of Clostridium Perfringens Epsilon-Toxin Is Induced by Activation of Neutral Sphingomyelinase  Biochim Biophys Acta  2016  1858(11):2681-2688  PubMed ID: 27453200   DOI: 10.1016/j.bbamem.2016.07.009
12209  R Hirose, T Daidoji, Y Naito, Y Watanabe, Y Arai 5, T Oda, H Konishi, M Yamawaki, Y Itoh, T Nakaya  Long-term detection of seasonal influenza RNA in faeces and intestine  Clin Microbiol Infect  2016  22(9):813.e1-813.e7  PubMed ID: 27424942   DOI: 10.1016/j.cmi.2016.06.015
14462  Ryosuke Takahashi and Takaharu Okajima  Comparison between power-law rheological parameters of living cells in frequency and time domains measured by atomic force microscopy  Japanese Journal of Applied Physics  2016  55    DOI: 10.7567/JJAP.55.08NB22
17108  Hirano T, Kikuchi T, Tode N, Santoso A, Yamada M, Mitsuhashi Y, Komatsu R, Kawabe T, Tanimoto T, Ishii N, Tanaka Y, Nishimura H, Nukiwa T, Watanabe A, Ichinose M.  OX40 ligand newly expressed on bronchiolar progenitors mediates influenza infection and further exacerbates pneumonia  EMBO Mol Med  2016  8(4):422-36  PubMed ID: 26976612   DOI: 10.15252/emmm.201506154
7165  Yoshizaki H, Kuwajima Y, Minato H, Kiyokawa E.  Regulation of Ripply1 expression in MDCK organoids.  Biochem. Biophys. Res. Commun.  2015  468:337-42  PubMed ID: 26514726   DOI: 10.1016/j.bbrc.2015.10.099
7286  Hosokawa-Muto J, Fujinami Y, Mizuno N.  Evaluation of the Universal Viral Transport system for long-term storage of virus specimens for microbial forensics.  J Forensic Leg Med  2015  34:29-33  PubMed ID: 26165655   DOI: 10.1016/j.jflm.2015.04.019
7485  Daidoji T, Watanabe Y, Ibrahim MS, Yasugi M, Maruyama H, Masuda T, Arai F, Ohba T, Honda A, Ikuta K, Nakaya T.  Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH.  J. Biol. Chem.  2015  290:10627-42  PubMed ID: 25673693   DOI: 10.1074/jbc.M114.611327
7534  K Furusawa, T Mizutani, H Machino, S Yahata, A Fukui, N Sasaki  Application of multichannel collagen gels in construction of epithelial lumen-like engineered tissues.  ACS Biomater Sci Eng  2015  1:539-548    DOI: 10.1021/acsbiomaterials.5b00003
9116  Masahiro Nagahama, Akiko Ohkubo, Yoshihito Kinouchi, Keiko Kobayashi, Kazuaki Miyamoto, Masaya Takehara, Jun Sakurai  Clostridium Perfringens TpeL Induces Formation of Stress Fibers via Activation of RhoA-ROCK Signaling Pathway  Biol Pharm Bull  2015  38 (5), 732-9  PubMed ID: 25947919   DOI: 10.1248/bpb.b14-00842
14957  Yamaguchi N, Mizutani T, Kawabata K, Haga H.  Leader cells regulate collective cell migration via Rac activation in the downstream signaling of integrin β1 and PI3K  Sci Rep  2015  5:7656  PubMed ID: 25563751   DOI: 10.1038/srep07656
14992  Imai M, Furusawa K, Mizutani T, Kawabata K, Haga H.  Three-dimensional morphogenesis of MDCK cells induced by cellular contractile forces on a viscous substrate  Sci Rep  2015  5:14208  PubMed ID: 26374384   DOI: 10.1038/srep14208
9107  Masahiro Nagahama, Chihiro Takahashi, Kouhei Aoyanagi, Ryo Tashiro, Keiko Kobayashi, Yoshihiko Sakaguchi, Kazumi Ishidoh, Jun Sakurai   Intracellular Trafficking of Clostridium Botulinum C2 Toxin  Toxicon  2014  82, 76-82  PubMed ID: 24582943   DOI: 10.1016/j.toxicon.2014.02.009
10262  Shin-Ichiro Miyashita, Koichi Niwa, Toshihiro Watanabe, Yoshimasa Sagane  Host-cell Specificity and Transcytosis of Nontoxic Nonhemagglutinin Protein of Botulinum Neurotoxin Serotype D  FEMS Microbiol Lett  2014  2014 Aug;357(2):115-22  PubMed ID: 25041523   DOI: 10.1111/1574-6968.12527
5338  Sogawa N, Hirai K, Sogawa C, Ohyama K, Miyazaki I, Tsukamoto G, Asanuma M, Sasaki A, Kitayama S.  Protective effect of cepharanthin on cisplatin-induced renal toxicity through metallothionein expression.  Life Sci  2013  92(12):727-32  PubMed ID: 23399700   DOI: 10.1016/j.lfs.2013.01.031
5340  Mori Y, Yagi S, Sakurai A, Matsuda M, Kiyokawa E.  Insufficient ability of Rac1b to perturb cystogenesis.  Small GTPases  2013  4(1):9-15  PubMed ID: 23411476   DOI: 10.4161/sgtp.23311
14193  Yu YC, Sohma Y, Takimoto S, Miyauchi T, Yasui M.  Direct visualization and quantitative analysis of water diffusion in complex biological tissues using CARS microscopy  Sci Rep  2013  3:2745  PubMed ID: 24067894   DOI: 10.1038/srep02745
17164  Hamamoto I, Takaku H, Tashiro M, Yamamoto N.  High yield production of influenza virus in Madin Darby canine kidney (MDCK) cells with stable knockdown of IRF7  PLoS One  2013  8(3):e59892  PubMed ID: 23555825   DOI: 10.1371/journal.pone.0059892
7635  Hosono Y, Yamaguchi T, Mizutani E, Yanagisawa K, Arima C, Tomida S, Shimada Y, Hiraoka M, Kato S, Yokoi K, Suzuki M, Takahashi T.  MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis.  EMBO J.  2012  31:481-93  PubMed ID: 22085929   DOI: 10.1038/emboj.2011.416
5332  Sakurai A, Matsuda M, Kiyokawa E.  Activated Ras protein accelerates cell cycle progression to perturb Madin-Darby canine kidney cystogenesis.  J Biol Chem  2012  287(38):31703-11  PubMed ID: 22829590   DOI: 10.1074/jbc.M112.377804
5335  Yagi S, Matsuda M, Kiyokawa E.  Chimaerin suppresses Rac1 activation at the apical membrane to maintain the cyst structure.  PLoS One  2012  7(12):e52258  PubMed ID: 23284959   DOI: 10.1371/journal.pone.0052258
5343  Komura K, Ise H, Akaike T.  Dynamic behaviors of vimentin induced by interaction with GlcNAc molecules.  Glycobiology  2012  22(12):1741-59  PubMed ID: 22846177   DOI: 10.1093/glycob/cws118
9090  Masahiro Nagahama, Mariko Umezaki, Ryo Tashiro, Masataka Oda, Keiko Kobayashi, Masahiro Shibutani, Teruhisa Takagishi, Kazumi Ishidoh, Mitsunori Fukuda, Jun Sakurai  Intracellular Trafficking of Clostridium Perfringens Iota-Toxin B  Infect Immun  2012  80 (10), 3410-6  PubMed ID: 22825447   DOI: 10.1128/IAI.00483-12
7743  Nagahama M, Itohayashi Y, Hara H, Higashihara M, Fukatani Y, Takagishi T, Oda M, Kobayashi K, Nakagawa I, Sakurai J.  Cellular vacuolation induced by Clostridium perfringens epsilon-toxin.  FEBS J.  2011  278:3395-407  PubMed ID: 21781280   DOI: 10.1111/j.1742-4658.2011.08263.x
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15672  Yamasaki T, Kawamura K, Hatori A, Yui J, Yanamoto K, Yoshida Y, Ogawa M, Nengaki N, Wakisaka H, Fukumura T, Zhang MR.  PET study on mice bearing human colon adenocarcinoma cells using [11C]GF120918, a dual radioligand for P-glycoprotein and breast cancer resistance protein  Nucl Med Commun  2010  31(11):985-93  PubMed ID: 20859232   DOI: 10.1097/MNM.0b013e32833fbf87
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15692  Yoshikawa F, Banno Y, Otani Y, Yamaguchi Y, Nagakura-Takagi Y, Morita N, Sato Y, Saruta C, Nishibe H, Sadakata T, Shinoda Y, Hayashi K, Mishima Y, Baba H, Furuichi T.  Phospholipase D family member 4, a transmembrane glycoprotein with no phospholipase D activity, expression in spleen and early postnatal microglia  PLoS One  2010  5(11):e13932  PubMed ID: 21085684   DOI: 10.1371/journal.pone.0013932
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8503  Nagahama M, Hagiyama T, Kojima T, Aoyanagi K, Takahashi C, Oda M, Sakaguchi Y, Oguma K, Sakurai J.  Binding and internalization of Clostridium botulinum C2 toxin.  Infect. Immun.  2009  77:5139-48  PubMed ID: 19720757   DOI: 10.1128/IAI.00638-09
8710  Sugibayashi K, Onuki Y, Takayama K.  Displacement of tight junction proteins from detergent-resistant membrane domains by treatment with sodium caprate.  Eur J Pharm Sci  2009  36:246-53  PubMed ID: 19013238   DOI: 10.1016/j.ejps.2008.09.011
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