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細胞番号 : 細胞名
RCB0992 : HOS  update : 2023/05/25
細胞特性(Comment:英)Sensitive to further transformation with both virus and chemicals. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 87070202 prior to order.
細胞特性(日)ヒト骨肉腫だが、扁平な細胞で、飽和密度も低い。ウイルスや発癌物質で更に悪性に転換する。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、 ECACC 87070202 を必ずご参照ください。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA87070202 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、 ECA87070202 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 ECACC
寄託日 1994
別名 TE 85, clone F-5
動物種 human < Mammals
属名 Homo
種名 sapiens
性別 Female
13 years
採取組織 bone
病名 Osteogenic sarcoma
細胞分類 cancer
入手歴 overseas, ECACC
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_0312
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% FBS + 0.1mM NEAA
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8-10 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
染色体検査 47-76(50) : 47(5),48(15),49(20),50(9),76(1)
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 3件
Reference(日) 0件
利用者成果(英) 31件
利用者成果(日) 0件

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Reference(英)
1827  Rhim, J S, Cho, H Y, Huebner, R J  Non-producer human cells induced by murine sarcoma virus.  Int J Cancer  1975  15:23-9  PubMed ID: 165148   DOI: 10.1002/ijc.2910150104
1828  Rhim, J S, Cho, H Y, Vernon, M L, Arnstein P, Huebner, R J, Gilden, R V  Characterization of non-producer human cells induced by Kirsten sarcoma virus.  Int J Cancer  1975  16:840-9  PubMed ID: 171229   DOI: 10.1002/ijc.2910160516
1829  McAllister, R M, Gardner, M B, Greene, A E, Bradt C, Nichols, W W, Landing, B H  Cultivation in vitro of cells derived from a human osteosarcoma.  Cancer  1971  27:397-402  PubMed ID: 5100401   DOI: 10.1002/1097-0142(197102)27:2<397::aid-cncr2820270224>3.0.co;2-x

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Reference(日)

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16215  Manami Suzuki-Karasaki, Takashi Ando, Yushi Ochiai, Kenta Kawahara, Miki Suzuki-Karasaki, Hideki Nakayama, Yoshihiro Suzuki-Karasaki  Air Plasma-Activated Medium Evokes a Death-Associated Perinuclear Mitochondrial Clustering  Int J Mol Sci  2022  23(3):1124  PubMed ID: 35163042   DOI: 10.3390/ijms23031124
20833  Tanaka K, Suzuki K, Miyashita K, Wakasa K, Kawano M, Nakatsu Y, Tsumura H, Yoshida MA, Oda S.  Activation of recombinational repair in Ewing sarcoma cells carrying EWS-FLI1 fusion gene by chromosome translocation.  Sci Rep  2022  12(1):14764  PubMed ID: 36042341   DOI: 10.1038/s41598-022-19164-x
13419  Suzuki S, Yuan H, Hirata-Tsuchiya S, Yoshida K, Sato A, Nemoto E, Shiba H, Yamada S.  DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.  Mol Cell Biochem  2021    PubMed ID: 33420898   DOI: 10.1007/s11010-020-04046-5
20341  Ouchi S, Niiyama E, Sugo K, Uto K, Takenaka S, Kikuchi A, Ebara M.  Shape-memory balloon offering simultaneous thermo/chemotherapies to improve anti-osteosarcoma efficacy.  Biomater Sci  2021    PubMed ID: 34546257   DOI: 10.1039/d1bm00780g
16865  Ando T, Suzuki-Karasaki M, Suzuki-Karasaki M, Ichikawa J, Ochiai T, Yoshida Y, Haro H, Suzuki-Karasaki Y.  Combined Anticancer Effect of Plasma-Activated Infusion and Salinomycin by Targeting Autophagy and Mitochondrial Morphology  Front Oncol  2021  11:593127  PubMed ID: 34150606   DOI: 10.3389/fonc.2021.593127
11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020  20(1):25.  PubMed ID: 31914969   DOI: 10.1186/s12885-019-6499-y
11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020  20(1):25.  PubMed ID: 31914969   DOI: 10.1186/s12885-019-6499-y
13057  Ichikawa J, Ando T, Kawasaki T, Sasaki T, Shirai T, Tsukiji N, Kimura Y, Aoki K, Hayakawa K, Suzuki-Inoue K, Saitoh M, Haro H.  Role of Platelet C-Type Lectin-Like Receptor 2 in Promoting Lung Metastasis in Osteosarcoma.  J Bone Miner Res  2020    PubMed ID: 32479683   DOI: 10.1002/jbmr.4045
13306  Ando T, Ichikawa J, Fujimaki T, Taniguchi N, Takayama Y, Haro H.  Gemcitabine and Rapamycin Exhibit Additive Effect against Osteosarcoma by Targeting Autophagy and Apoptosis.  Cancers (Basel)  2020    PubMed ID: 33114161   DOI: 10.3390/cancers12113097
13527  Takashi Ando, Manami Suzuki-Karasaki, Miki Suzuki-Karasaki, Jiro Ichikawa, Toyoko Ochiai, Yukihiro Yoshida, Hirotaka Haro, Yoshihiro Suzuki-Karasaki  Synergistic anticancer effect of plasma-activated infusion and salinomycin by targeting autophagy and mitochondrial morphology  bioRxiv  2020  October 15    DOI: 10.1101/2020.10.14.340208
14841  Fujikawa I, Ando T, Suzuki-Karasaki M, Suzuki-Karasaki M, Ochiai T, Suzuki-Karasaki Y  Aspirin Induces Mitochondrial Ca 2+ Remodeling in Tumor Cells via ROS‒Depolarization‒Voltage-Gated Ca 2+ Entry  Int J Mol Sci  2020  21(13):4771  PubMed ID: 32635638   DOI: 10.3390/ijms21134771
11978  Asano N, Takeshima H, Yamashita S, Takamatsu H, Hattori N, Kubo T, Yoshida A, Kobayashi E, Nakayama R, Matsumoto M, Nakamura M, Ichikawa H, Kawai A, Kondo T, Ushijima T.  Epigenetic reprogramming underlies efficacy of DNA demethylation therapy in osteosarcomas.  Sci Rep  2019    PubMed ID: 31889115   DOI: 10.1038/s41598-019-56883-0
12453  Kubo S, Takagi-Kimura M, Kasahara N.  Efficient tumor transduction and antitumor efficacy in experimental human osteosarcoma using retroviral replicating vectors  Cancer Gene Ther  2019  ;26(1-2):41-47  PubMed ID: 30042500   DOI: 10.1038/s41417-018-0037-y
10773  Yamada N, Kato-Kogoe N, Yamanegi K, Nishiura H, Fujihara Y, Fukunishi S, Okamura H, Terada N, Nakasho K.  Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.  Anticancer Res.  2018  38:1353-1359  PubMed ID: 29491059   DOI: 10.21873/anticanres.12358
4234  Ito T, Ando T, Suzuki-Karasaki M, Tokunaga T, Yoshida Y, Ochiai T, Tokuhashi Y, Suzuki-Karasaki Y.  Cold PSM, but not TRAIL, triggers autophagic cell death: A therapeutic advantage of PSM over TRAIL.  Int. J. Oncol.  2018    PubMed ID: 29845256   DOI: 10.3892/ijo.2018.4413
9465  Tomohiko Tokunaga, Takashi Ando, Miki Suzuki-Karasaki, Tomohisa Ito, Asuka Onoe-Takahashi, Toyoko Ochiai, Masayoshi Soma , Yoshihiro Suzuki-Karasaki  Plasma-stimulated Medium Kills TRAIL-resistant Human Malignant Cells by Promoting Caspase-Independent Cell Death via Membrane Potential and Calcium Dynamics Modulation  Int J Oncol  2018  52(3):697-708  PubMed ID: 29393427   DOI: 10.3892/ijo.2018.4251
14901  Enjoji S, Yabe R, Tsuji S, Yoshimura K, Kawasaki H, Sakurai M, Sakai Y, Takenouchi H, Yoshino S, Hazama S, Nagano H, Oshima H, Oshima M, Vitek MP, Matsuura T, Hippo Y, Usui T, Ohama T, Sato K.  Stemness Is Enhanced in Gastric Cancer by a SET/PP2A/E2F1 Axis  Mol Cancer Res  2018  16(3):554-563  PubMed ID: 29330298   DOI: 10.1158/1541-7786.MCR-17-0393
10919    Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma  Integr Mol Med  2016  3: 755-760    DOI: 10.15761/IMM.1000239
14959  Iwasaki T, Tanaka K, Kawano M, Itonaga I, Tsumura H.  Tumor-suppressive microRNA-let-7a inhibits cell proliferation via targeting of E2F2 in osteosarcoma cells  Int J Oncol  2015  46(4):1543-50  PubMed ID: 25647078   DOI: 10.3892/ijo.2015.2867
15802  Takagi-Kimura M, Yamano T, Tagawa M, Kubo S.  Oncolytic virotherapy for osteosarcoma using midkine promoter-regulated adenoviruses  Cancer Gene Ther  2014  21(3):126-32  PubMed ID: 24577130   DOI: 10.1038/cgt.2014.7
17112  Natsuko Ito, Masanobu Kamitakahara, Koji Ioku  Preparation and evaluation of spherical porous granules of octacalcium phosphate/hydroxyapatite as drug carriers in bone cancer treatment  Materials Letters  2014  120:94-96    DOI: 10.1016/j.matlet.2014.01.040
13804  Inoue T, Hagiyama M, Enoki E, Sakurai MA, Tan A, Wakayama T, Iseki S, Murakami Y, Fukuda K, Hamanishi C, Ito A.  Cell adhesion molecule 1 is a new osteoblastic cell adhesion molecule and a diagnostic marker for osteosarcoma  Life Sci  2013  92(1):91-9  PubMed ID: 23142238   DOI: 10.1016/j.lfs.2012.10.021
7838  Tsunashima Y, Kondo A, Matsuda T, Togari A.  Hydrocortisone inhibits cellular proliferation by downregulating hepatocyte growth factor synthesis in human osteoblasts.  Biol. Pharm. Bull.  2011  34:700-3  PubMed ID: 21532160   DOI: 10.1248/bpb.34.700
8031  Iizuka S, Kudo Y, Yoshida M, Tsunematsu T, Yoshiko Y, Uchida T, Ogawa I, Miyauchi M, Takata T.  Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.  Mol. Cell. Biol.  2011  31:783-92  PubMed ID: 21149578   DOI: 10.1128/MCB.00912-10
8056  Hori T, Kondo T, Lee H, Song CW, Park HJ.  Hyperthermia enhances the effect of β-lapachone to cause γH2AX formations and cell death in human osteosarcoma cells.  Int J Hyperthermia  2011  27:53-62  PubMed ID: 21070139   DOI: 10.3109/02656736.2010.513361
8261  Matsuda T, Kondo A, Tsunashima Y, Togari A.  Inhibitory effect of vitamin K(2) on interleukin-1beta-stimulated proliferation of human osteoblasts.  Biol. Pharm. Bull.  2010  33:804-8  PubMed ID: 20460758   DOI: 10.1248/bpb.33.804
8542  Hori T, Kondo T, Kanamori M, Tabuchi Y, Ogawa R, Zhao QL, Ahmed K, Yasuda T, Seki S, Suzuki K, Kimura T.  Nutlin-3 enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through up-regulation of death receptor 5 (DR5) in human sarcoma HOS cells and human colon cancer HCT116 cells.  Cancer Lett.  2010  287:98-108  PubMed ID: 19577358   DOI: 10.1016/j.canlet.2009.06.002
15673  Nakashima Y, Deie M, Yanada S, Sharman P, Ochi M.  Magnetically labeled human natural killer cells, accumulated in vitro by an external magnetic force, are effective against HOS osteosarcoma cells  Int J Oncol  2005  27(4):965-71  PubMed ID: 16142312  
3041  Terasaki, Tadashi, Kyo, Satoru, Takakura, Masahiro, Maida, Yoshiko, Tsuchiya, Hiroyuki, Tomita, Katsuro, Inoue, Masaki  Analysis of telomerase activity and telomere length in bone and soft tissue tumors.  Oncol Rep  2004  11:1307-11  PubMed ID: 15138570  
3962  Yoshiko Y, Son A, Maeda S, Igarashi A, Takano S, Hu J, Maeda N.  Evidence for stanniocalcin gene expression in mammalian bone.  Endocrinology  1999  140(4):1869-74  PubMed ID: 10098526   DOI: 10.1210/endo.140.4.6662
3970  Kashima T, Kawaguchi J, Takeshita S, Kuroda M, Takanashi M, Horiuchi H, Imamura T, Ishikawa Y, Ishida T, Mori S, Machinami R, Kudo A.  Anomalous cadherin expression in osteosarcoma. Possible relationships to metastasis and morphogenesis.  Am J Pathol  1999  155(5):1549-55  PubMed ID: 10550312   DOI: 10.1016/S0002-9440(10)65471-5

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