細胞番号 : 細胞名
RCB0991 : G-361
update : 2023/06/30
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| 細胞特性(Comment:英) | Human melanoma producing melanin up to 50 PDL. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 88030401 prior to order. |
| 細胞特性(日) | ヒト悪性黒色腫。初期 passage (50PDL程度)まではメラニン産生能を維持している。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、 ECACC 88030401 を必ずご参照ください。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88030401 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、 ECA88030401 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
ECACC
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| 寄託日 |
1994
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Male
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| 齢 |
31 years
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| 採取組織 |
skin
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| 病名 |
malignant melanoma
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| 細胞分類 |
cancer
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| 入手歴 |
overseas, ECACC
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|
細胞寿命 |
infinite
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| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_1220
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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McCoy's 5A + 10% FBS
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| 抗生物質 |
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Free
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継代方法 |
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pipetting
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継代密度 |
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1 : 8 split
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| 継代・培地交換頻度 |
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Subculture : once/week, Medium Renewal : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| ウィルス（HIV) |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
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65-133(50) : /67(3),68(5),69(13),70(7),71(6),72(4)/
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
1件
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| Reference(日) |
0件
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| 利用者成果(英) |
20件
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| 利用者成果(日) |
0件
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| 利用者成果(英) |
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17336
Takahiro Oyama, Atsushi Yoshimori, Haruka Ogawa, Yoko Shirai, Hideaki Abe, Takanori Kamiya, Sei-ichi Tanuma
The structural differences between mushroom and human tyrosinase cleared by investigating the inhibitory activities of stilbenes
Journal of Molecular Structure
2023
1272
DOI: 10.1016/j.molstruc.2022.134180
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20334
Oyama T, Ogawa H, Shirai Y, Abe H, Kamiya T, Abe T, Tanuma SI.
Hinokitiol-induced decreases of tyrosinase and microphthalmia-associated transcription factor are mediated by the endoplasmic reticulum-associated degradation pathway in human melanoma cells.
Biochimie
2022
PubMed ID: 34536557
DOI: 10.1016/j.biochi.2021.09.007
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20774
Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.
Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.
Nat Commun
2022
13(1):4063
PubMed ID: 35831322
DOI: 10.1038/s41467-022-31690-w
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20898
Koizumi K, Shintani T, Hayashido Y, Hamada A, Higaki M, Yoshioka Y, Sakamoto A, Yanamoto S, Okamoto T.
VEGF-A promotes the motility of human melanoma cells through the VEGFR1-PI3K/Akt signaling pathway.
In Vitro Cell Dev Biol Anim
2022
58(8):758-770
PubMed ID: 35997849
DOI: 10.1007/s11626-022-00717-3
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13894
Andoh T, Fujimoto T, Satani R, Suzuki M, Wada K, Sudo T, Sakurai Y, Tanaka H, Takata T, Ichikawa H.
Preclinical study of boron neutron capture therapy for bone metastasis using human breast cancer cell lines
Appl Radiat Isot
2020
165:109257.
PubMed ID: 32777739
DOI: 10.1016/j.apradiso.2020.109257
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4187
Fujiwara S, Nagai H, Jimbo H, Jimbo N, Tanaka T, Inoie M, Nishigori C.
Gene Expression and Methylation Analysis in Melanomas and Melanocytes From the Same Patient: Loss of NPM2 Expression Is a Potential Immunohistochemical Marker for Melanoma.
Front Oncol
2019
8:675
PubMed ID: 30719424
DOI: 10.3389/fonc.2018.00675
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11645
Yamada H, Hakozaki M, Uemura A, Yamashita T.
Effect of fatty acids on melanogenesis and tumor cell growth in melanoma cells.
J. Lipid Res.
2019
PubMed ID: 31345992
DOI: 10.1194/jlr.M090712
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12521
Hayashi Y, Jia W, Kidoya H, Muramatsu F, Tsukada Y, Takakura N.
Galectin-3 Inhibits Cancer Metastasis by Negatively Regulating Integrin β3 Expression
Am J Pathol
2019
189(4):900-910
PubMed ID: 30653955
DOI: 10.1016/j.ajpath.2018.12.005
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14488
Nobeyama Y, Nakagawa H.
Silencing of interferon regulatory factor gene 6 in melanoma
PLoS One
2017
12(9):e0184444
PubMed ID: 28877249
DOI: 10.1371/journal.pone.0184444
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14489
Nobeyama Y, Nakagawa H.
Silencing of metallothionein 1A gene in melanoma
J Dermatol Sci
2017
88(2):232-237
PubMed ID: 28764861
DOI: 10.1016/j.jdermsci.2017.07.009
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15056
Iijima M, Kubota Y, Sawa R, Kubota Y, Hatano M, Igarashi M, Kawada M, Momose I, Takekawa M, Shibasaki M.
A guanine derivative as a new MEK inhibitor produced by Streptomyces sp. MK63-43F2
J Antibiot (Tokyo)
2017
Sep 6
PubMed ID: 28874847
DOI: 10.1038/ja.2017.100
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7303
Kobayashi H, Nobeyama Y, Nakagawa H.
Tumor-suppressive effects of natural-type interferon-β through CXCL10 in melanoma.
Biochem. Biophys. Res. Commun.
2015
464:416-21
PubMed ID: 26102027
DOI: 10.1016/j.bbrc.2015.06.122
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10291
Katsuhiro Uzawa, Atsushi Kasamatsu, Toshihiro Shimizu, Yasuhiro Saito, Takao Baba, Kentaro Sakuma, Kazuaki Fushimi, Yosuke Sakamoto, Katsunori Ogawara, Masashi Shiiba, Hideki Tanzawa
Suppression of Metastasis by Mirtazapine via Restoration of the Lin-7C/β-catenin Pathway in Human Cancer Cells
Sci Rep
2014
4:5433
PubMed ID: 24961284
DOI: 10.1038/srep05433
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16001
Yanagishita T, Yajima I, Kumasaka M, Kawamoto Y, Tsuzuki T, Matsumoto Y, Watanabe D, Kato M.
Actin-binding protein, Espin: a novel metastatic regulator for melanoma
Mol Cancer Res
2014
12(3):440-6
PubMed ID: 24337068
DOI: 10.1158/1541-7786.MCR-13-0468-T
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7610
Thang ND, Yajima I, Nakagawa K, Tsuzuki T, Kumasaka MY, Ohgami N, Ly TB, Iwamoto T, Watanabe D, Kato M.
A novel hairless mouse model for malignant melanoma.
J. Dermatol. Sci.
2012
65:207-12
PubMed ID: 22169325
DOI: 10.1016/j.jdermsci.2011.10.010
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18797
Saito A, Saito N, Mol W, Furukawa H, Tsutsumida A, Oyama A, Sekido M, Sasaki S, Yamamoto Y.
Simvastatin inhibits growth via apoptosis and the inducti
Melanoma Res
2008
18(2):85-94
PubMed ID: 18337644
DOI: 10.1097/CMR.0b013e3282f60097
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9063
Daisuke Watanabe, Fumi Goshima, Isamu Mori, Yasuhiko Tamada, Yoshinari Matsumoto, Yukihiro Nishiyama
Oncolytic Virotherapy for Malignant Melanoma With Herpes Simplex Virus Type 1 Mutant HF10
J Dermatol Sci
2008
50 (3), 185-96
PubMed ID: 18226503
DOI: 10.1016/j.jdermsci.2007.12.001
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17377
Nakamura S, Inui M, Kamei T, Nakase M, Okumura K, Tagawa T.
G3139 induces cell death by caspase-dependent and -independent apoptosis on human melanoma cell lines
Oncol Rep
2006
15(6):1563-8
PubMed ID: 16685396
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4448
Nagata S, Obana A, Gohto Y, Nakajima S.
Necrotic and apoptotic cell death of human malignant melanoma cells following photodynamic therapy using an amphiphilic photosensitizer, ATX-S10(Na)
Lasers Surg Med
2003
33(1):64-70.
PubMed ID: 12866123
DOI: 10.1002/lsm.10190
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13889
Furukawa Y, Nishimura N, Furukawa Y, Satoh M, Endo H, Iwase S, Yamada H, Matsuda M, Kano Y, Nakamura M.
Apaf-1 is a mediator of E2F-1-induced apoptosis
J Biol Chem
2002
277(42):39760-8
PubMed ID: 12149244
DOI: 10.1074/jbc.M200805200
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