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Cell No. : Cell Name
RCB0991 : G-361  update : 2022/03/25
CommentHuman melanoma producing melanin up to 50 PDL. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88030401 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88030401 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 1994
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 31 years
Tissue skin
Disease name malignant melanoma
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1220
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives McCoy's 5A + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 65-133(50) : /67(3),68(5),69(13),70(7),71(6),72(4)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 17


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Reference
125  Paul T Peebles, Timothy Trisch & Alex G Papageorge   727 ISOLATION OF FOUR UNUSUAL PEDIATRIC SOLID TUMOR CELL LINES  Pediatric Research  1978  12:485    DOI: 10.1203/00006450-197804001-00732

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User's Publication
20334  Oyama T, Ogawa H, Shirai Y, Abe H, Kamiya T, Abe T, Tanuma SI.  Hinokitiol-induced decreases of tyrosinase and microphthalmia-associated transcription factor are mediated by the endoplasmic reticulum-associated degradation pathway in human melanoma cells.  Biochimie  2022    PubMed ID: 34536557   DOI: 10.1016/j.biochi.2021.09.007
20774  Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.  Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.  Nat Commun  2022  13(1):4063  PubMed ID: 35831322   DOI: 10.1038/s41467-022-31690-w
20898  Koizumi K, Shintani T, Hayashido Y, Hamada A, Higaki M, Yoshioka Y, Sakamoto A, Yanamoto S, Okamoto T.  VEGF-A promotes the motility of human melanoma cells through the VEGFR1-PI3K/Akt signaling pathway.  In Vitro Cell Dev Biol Anim  2022  58(8):758-770  PubMed ID: 35997849   DOI: 10.1007/s11626-022-00717-3
13894  Andoh T, Fujimoto T, Satani R, Suzuki M, Wada K, Sudo T, Sakurai Y, Tanaka H, Takata T, Ichikawa H.  Preclinical study of boron neutron capture therapy for bone metastasis using human breast cancer cell lines  Appl Radiat Isot  2020  165:109257.  PubMed ID: 32777739   DOI: 10.1016/j.apradiso.2020.109257
4187  Fujiwara S, Nagai H, Jimbo H, Jimbo N, Tanaka T, Inoie M, Nishigori C.  Gene Expression and Methylation Analysis in Melanomas and Melanocytes From the Same Patient: Loss of NPM2 Expression Is a Potential Immunohistochemical Marker for Melanoma.  Front Oncol  2019  8:675  PubMed ID: 30719424   DOI: 10.3389/fonc.2018.00675
11645  Yamada H, Hakozaki M, Uemura A, Yamashita T.  Effect of fatty acids on melanogenesis and tumor cell growth in melanoma cells.  J. Lipid Res.  2019    PubMed ID: 31345992   DOI: 10.1194/jlr.M090712
12521  Hayashi Y, Jia W, Kidoya H, Muramatsu F, Tsukada Y, Takakura N.  Galectin-3 Inhibits Cancer Metastasis by Negatively Regulating Integrin β3 Expression  Am J Pathol  2019  189(4):900-910  PubMed ID: 30653955   DOI: 10.1016/j.ajpath.2018.12.005
14488  Nobeyama Y, Nakagawa H.  Silencing of interferon regulatory factor gene 6 in melanoma  PLoS One  2017  12(9):e0184444  PubMed ID: 28877249   DOI: 10.1371/journal.pone.0184444
14489  Nobeyama Y, Nakagawa H.  Silencing of metallothionein 1A gene in melanoma  J Dermatol Sci  2017  88(2):232-237  PubMed ID: 28764861   DOI: 10.1016/j.jdermsci.2017.07.009
15056  Iijima M, Kubota Y, Sawa R, Kubota Y, Hatano M, Igarashi M, Kawada M, Momose I, Takekawa M, Shibasaki M.  A guanine derivative as a new MEK inhibitor produced by Streptomyces sp. MK63-43F2  J Antibiot (Tokyo)  2017  Sep 6  PubMed ID: 28874847   DOI: 10.1038/ja.2017.100
7303  Kobayashi H, Nobeyama Y, Nakagawa H.  Tumor-suppressive effects of natural-type interferon-β through CXCL10 in melanoma.  Biochem. Biophys. Res. Commun.  2015  464:416-21  PubMed ID: 26102027   DOI: 10.1016/j.bbrc.2015.06.122
10291  Katsuhiro Uzawa, Atsushi Kasamatsu, Toshihiro Shimizu, Yasuhiro Saito, Takao Baba, Kentaro Sakuma, Kazuaki Fushimi, Yosuke Sakamoto, Katsunori Ogawara, Masashi Shiiba, Hideki Tanzawa   Suppression of Metastasis by Mirtazapine via Restoration of the Lin-7C/β-catenin Pathway in Human Cancer Cells  Sci Rep  2014  4:5433  PubMed ID: 24961284   DOI: 10.1038/srep05433
16001  Yanagishita T, Yajima I, Kumasaka M, Kawamoto Y, Tsuzuki T, Matsumoto Y, Watanabe D, Kato M.  Actin-binding protein, Espin: a novel metastatic regulator for melanoma  Mol Cancer Res  2014  12(3):440-6  PubMed ID: 24337068   DOI: 10.1158/1541-7786.MCR-13-0468-T
7610  Thang ND, Yajima I, Nakagawa K, Tsuzuki T, Kumasaka MY, Ohgami N, Ly TB, Iwamoto T, Watanabe D, Kato M.  A novel hairless mouse model for malignant melanoma.  J. Dermatol. Sci.  2012  65:207-12  PubMed ID: 22169325   DOI: 10.1016/j.jdermsci.2011.10.010
9063  Daisuke Watanabe, Fumi Goshima, Isamu Mori, Yasuhiko Tamada, Yoshinari Matsumoto, Yukihiro Nishiyama  Oncolytic Virotherapy for Malignant Melanoma With Herpes Simplex Virus Type 1 Mutant HF10  J Dermatol Sci  2008  50 (3), 185-96  PubMed ID: 18226503   DOI: 10.1016/j.jdermsci.2007.12.001
4448  Nagata S, Obana A, Gohto Y, Nakajima S.  Necrotic and apoptotic cell death of human malignant melanoma cells following photodynamic therapy using an amphiphilic photosensitizer, ATX-S10(Na)  Lasers Surg Med  2003  33(1):64-70.  PubMed ID: 12866123   DOI: 10.1002/lsm.10190
13889  Furukawa Y, Nishimura N, Furukawa Y, Satoh M, Endo H, Iwase S, Yamada H, Matsuda M, Kano Y, Nakamura M.  Apaf-1 is a mediator of E2F-1-induced apoptosis  J Biol Chem  2002  277(42):39760-8  PubMed ID: 12149244   DOI: 10.1074/jbc.M200805200



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