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Cell No. : Cell Name
RCB0990 : CPAE  update : 2021/05/20
CommentBovine pulmonary artery endothelial cells producing angiotensin-converting enzyme. Bovine Viral Diarrhea Virus (BVDV) positive. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86111401 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86111401 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 1994
Animal _bovine < Mammals
Genus Bos
Species taurus
Gender Female
Tissue pulmonary artery endothelium
Classification transformed
History overseas, ECACC
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2877
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 20% FBS + 0.1mM NEAA
Antibiotics Free
Passage method (0.05% trypsin + 0.02% EDTA) or (0.25% trypsin + 0.02% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
15541  Susumu Muroya, Ikuyo Nakajima, Koichi Chikuni  Bovine Skeletal Muscle Cells Predominantly Express a Vascular Cell Adhesion Molecule-1 Seven-Ig Domain Splice Form  Zoological Science  2001  18:797-805 



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