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Cell No. : Cell Name
RCB0989 : COLO 679  update : 2023/06/30
CommentMelanoma from a 47 year old female. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 87061210 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA87061210 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 1994
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 47 years
Tissue skin
Disease name melanima
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1130
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 20% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 42-88(50) : /44(3),45(7),46(17),47(18),48(1),73(1)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 19


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Reference

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User's Publication
19811  Une S, Nakata R, Nonaka K, Akiyama J.  Antiproliferative and anti-inflammatory effects of fractionated crude lectins from boiled kidney beans (Phaseolus vulgaris)  J Food Sci  2024  89(1):671-683  PubMed ID: 37983860   DOI: 10.1111/1750-3841.16847
22098  Acra AM, Uota S, Yoshihara M, Murakami Y, Sakagami H.  Potential Medicinal Efficacy of Alkaline Extract of Pine Seed Shell: Anti-UVC Activity and Macrophage Activation.  In Vivo  2024  38(6):2629-2638  PubMed ID: 39477410   DOI: 10.21873/invivo.13739
21460  Otaka Y, Izawa M, Sakagami H, Shiba N, Takahashi N, Tanuma SI, Amano S, Uota S, Inomata M, Yokose S, Sunaga K, Hayashi S, Koga-Ogawa Y, Nakaya G, Kito S.  UVC-Protective Activity of Lemongrass Among 12 Fat-soluble Herbal Extracts: Rapid Decay Due to Cytotoxicity.  In Vivo  2023  37(6):2464-2472  PubMed ID: 37905640   DOI: 10.21873/invivo.13353
20766  Tachibana K, Ohkawa Y, Kanto N, Maeda K, Ohe S, Isei T, Harada Y, Taniguchi N.  The expression of keratan sulfate in malignant melanoma enhances the adhesion and invasion activity of melanoma cells.  J Dermatol  2022    PubMed ID: 35811379   DOI: 10.1111/1346-8138.16506
20909  Sakagami H, Amano S, Uota S, Tanuma SI, Inomata M, Shindo A, Kusano M, Kikkawa Y, Horiuchi M, Ooka T.  Prominent Anti-UVC Activity of Lignin Degradation Products.  In Vivo  2022  36(6):2689-2699  PubMed ID: 36309360   DOI: 10.21873/invivo.13004
11645  Yamada H, Hakozaki M, Uemura A, Yamashita T.  Effect of fatty acids on melanogenesis and tumor cell growth in melanoma cells.  J. Lipid Res.  2019    PubMed ID: 31345992   DOI: 10.1194/jlr.M090712
6893  Ogawa M, Yaginuma T, Nakatomi C, Nakajima T, Tada-Shigeyama Y, Addison WN, Urata M, Matsubara T, Watanabe K, Matsuo K, Sato T, Honda H, Hikiji H, Watanabe S, Kokabu S.  Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity.  Oncotarget  2019  10(3):404-414  PubMed ID: 30719233   DOI: 10.18632/oncotarget.26552
12351  Masuda H, Kimura M, Nishioka A, Kato H, Morita A.  Dual wavelength 5-aminolevulinic acid photodynamic therapy using a novel flexible light-emitting diode unit  J Dermatol Sci  2019  93(2):109-115  PubMed ID: 30704937   DOI: 10.1016/j.jdermsci.2018.12.006
14901  Enjoji S, Yabe R, Tsuji S, Yoshimura K, Kawasaki H, Sakurai M, Sakai Y, Takenouchi H, Yoshino S, Hazama S, Nagano H, Oshima H, Oshima M, Vitek MP, Matsuura T, Hippo Y, Usui T, Ohama T, Sato K.  Stemness Is Enhanced in Gastric Cancer by a SET/PP2A/E2F1 Axis  Mol Cancer Res  2018  16(3):554-563  PubMed ID: 29330298   DOI: 10.1158/1541-7786.MCR-17-0393
13651  Kito Y, Bai J, Goto N, Okubo H, Adachi Y, Nagayama T, Takeuchi T.  Pathobiological properties of the ubiquitin ligase Nedd4L in melanoma  Int J Exp Pathol  2014  95(1):24-8  PubMed ID: 24456330   DOI: 10.1111/iep.12051
16001  Yanagishita T, Yajima I, Kumasaka M, Kawamoto Y, Tsuzuki T, Matsumoto Y, Watanabe D, Kato M.  Actin-binding protein, Espin: a novel metastatic regulator for melanoma  Mol Cancer Res  2014  12(3):440-6  PubMed ID: 24337068   DOI: 10.1158/1541-7786.MCR-13-0468-T
7610  Thang ND, Yajima I, Nakagawa K, Tsuzuki T, Kumasaka MY, Ohgami N, Ly TB, Iwamoto T, Watanabe D, Kato M.  A novel hairless mouse model for malignant melanoma.  J. Dermatol. Sci.  2012  65:207-12  PubMed ID: 22169325   DOI: 10.1016/j.jdermsci.2011.10.010
13479  Matsumoto Y, Iwakawa M, Furusawa Y, Ishikawa K, Aoki M, Imadome K, Matsumoto I, Tsujii H, Ando K, Imai T.  Gene expression analysis in human malignant melanoma cell lines exposed to carbon beams  Int J Radiat Biol  2008  84(4):299-314  PubMed ID: 18386195   DOI: 10.1080/09553000801953334
3689  Nobeyama Y, Okochi-Takada E, Furuta J, Miyagi Y, Kikuchi K, Yamamoto A, Nakanishi Y, Nakagawa H, Ushijima T.  Silencing of tissue factor pathway inhibitor-2 gene in malignant melanomas.  Int J Cancer  2007  121(2):301-7  PubMed ID: 17372906   DOI: 10.1002/ijc.22637
3445  Furuta, Junichi, Nobeyama, Yoshimasa, Umebayashi, Yoshihiro, Otsuka, Fujio, Kikuchi, Kanako, Ushijima, Toshikazu  Silencing of Peroxiredoxin 2 and aberrant methylation of 33 CpG islands in putative promoter regions in human malignant melanomas.  Cancer Res  2006  66:6080-6  PubMed ID: 16778180   DOI: 10.1158/0008-5472.CAN-06-0157
6448  Takeuchi T, Adachi Y, Sonobe H, Furihata M, Ohtsuki Y.  A ubiquitin ligase, skeletrophin, is a negative regulator of melanoma invasion.  Oncogene  2006  25(53):7059-69  PubMed ID: 16715130   DOI: 10.1038/sj.onc.1209688
3093  Furuta, Junichi, Kaneda, Atsushi, Umebayashi, Yoshihiro, Otsuka, Fujio, Sugimura, Takashi, Ushijima, Toshikazu  Silencing of the thrombomodulin gene in human malignant melanoma.  Melanoma Res  2005  15:15-20  PubMed ID: 15714116   DOI: 10.1097/00008390-200502000-00004
15609  Furuta J, Umebayashi Y, Miyamoto K, Kikuchi K, Otsuka F, Sugimura T, Ushijima T.  Promoter methylation profiling of 30 genes in human malignant melanoma  Cancer Sci   2004  95(12):962-8  PubMed ID: 15596045   DOI: 10.1111/j.1349-7006.2004.tb03184.x
13889  Furukawa Y, Nishimura N, Furukawa Y, Satoh M, Endo H, Iwase S, Yamada H, Matsuda M, Kano Y, Nakamura M.  Apaf-1 is a mediator of E2F-1-induced apoptosis  J Biol Chem  2002  277(42):39760-8  PubMed ID: 12149244   DOI: 10.1074/jbc.M200805200


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