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Cell No. : Cell Name
RCB0983 : ECC10  update : 2024/02/27
CommentHuman cell line derived from stomach cancer (small cell cancer). Expressing creatinine kinase of brain isoenzyme etc. ( PubMed ID: 8392984).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Motoyama, Teiichi
Originator Ishihara, Noriko
Year of deposit 1994
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue stomach, liver meta
Disease name small-cell gastrointestinal
Metastatic ability Yes
Metastatic tissue liver, lymph node
Classification cancer
History originator
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1188
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Chromosome mode 60-164(50) : /78(5),79(2),80(4),81(2),82(7)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 13

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11693  T Fujiwara, T Motoyama, N Ishihara, H Watanabe, T Kumanishi, K Kato, H Ichinose, T Nagatsu  Characterization of four new cell lines derived from small-cell gastrointestinal carcinoma  Int J Cancer  1993  54(6):965-71  PubMed ID: 8392984   DOI: 10.1002/ijc.2910540617

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User's Publication
20739  Chen P, Wang W, Wong SW, Li J, Wu Q, Zhang SD, Lin Y, Kwok HF.  RUNDC3A regulates SNAP25-mediated chemotherapy resistance by binding AKT in gastric neuroendocrine carcinoma (GNEC).  Cell Death Discov  2022  8(1):296  PubMed ID: 35752613   DOI: 10.1038/s41420-022-01084-4
13415  Yamada K, Hori Y, Inoue S, Yamamoto Y, Iso K, Kamiyama H, Yamaguchi A, Kimura T, Uesugi M, Ito J, Matsuki M, Nakamoto K, Harada H, Yoneda N, Takemura A, Kushida I, Wakayama N, Kubara K, Kato Y, Semba T, Yokoi A, Matsukura M, Odagami T, Iwata M, Tsuruoka A, Uenaka T, Matsui J, Matsushima T, Nomoto K, Kouji H, Owa T, Funahashi Y, Ozawa Y.  E7386, a selective inhibitor of the interaction between β-catenin and CBP, exerts antitumor activity in tumor models with activated canonical Wnt signaling.  Cancer Res  2021    PubMed ID: 33408116   DOI: 10.1158/0008-5472.CAN-20-0782
13233  Xie J, Chen P, Xie H, Sun Y, Huang Z, Wei R, Miao Z, Wang Q, Zhang SD, Wong KH, Lin Y, Huang C, Kwok HF.  Exploration of gastric neuroendocrine carcinoma (GNEC) specific signaling pathways involved in chemoresistance via transcriptome and in vitro analysis.  Comput Struct Biotechnol J  2020    PubMed ID: 33033581   DOI: 10.1016/j.csbj.2020.09.016
6405  Matsuo K, Taniguchi K, Hamamoto H, Ito Y, Futaki S, Inomata Y, Shima T, Asakuma M, Lee SW, Tanaka K, Okuda J, Kondo Y, Uchiyama K.  Delta-like 3 localizes to neuroendocrine cells and plays a pivotal role in gastrointestinal neuroendocrine malignancy.  Cancer Sci  2019  110(10):3122-3131  PubMed ID: 31369178   DOI: 10.1111/cas.14157
6413  Lin JX, Weng XF, Xie XS, Lian NZ, Qiu SL, Wang JB, Lu J, Chen QY, Cao LL, Lin M, Tu RH, Yang YH, Liu SJ, Hu M, Lin YK, Huang CM, Zheng CH, Li P, Xie JW.  CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling.  Cancer Cell Int  2019  7;19:282  PubMed ID: 31728130   DOI: 10.1186/s12935-019-0997-5
11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217   DOI: 10.1016/j.bbrep.2019.100632
4380  Yoshioka T, Shien K, Namba K, Torigoe H, Sato H, Tomida S, Yamamoto H, Asano H, Soh J, Tsukuda K, Nagasaka T, Fujiwara T, Toyooka S.  Antitumor activity of pan-HER inhibitors in HER2-positive gastric cancer.  Cancer Sci.  2018  109(4):1166-1176  PubMed ID: 29465762   DOI: 10.1111/cas.13546
6411  Shiimura Y, Ohgusu H, Sato T, Kojima M.  Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2.  Int J Endocrinol  2015  2015:580908  PubMed ID: 25699080   DOI: 10.1155/2015/580908
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
6412  Shida T, Furuya M, Nikaido T, Hasegawa M, Koda K, Oda K, Miyazaki M, Kishimoto T, Nakatani Y, Ishikura H.  Sonic Hedgehog-Gli1 signaling pathway might become an effective therapeutic target in gastrointestinal neuroendocrine carcinomas.  Cancer Biol Ther  2006  5(11):1530-8  PubMed ID: 17102592   DOI: 10.4161/cbt.5.11.3458
6414  Yeung CM, Chan CB, Woo NY, Cheng CH.  Seabream ghrelin: cDNA cloning, genomic organization and promoter studies.  J Endocrinol  2006  189(2):365-79  PubMed ID: 16648303   DOI: 10.1677/joe.1.06593
6408  Yoshino N, Ishihara S, Rumi MA, Ortega-Cava CF, Yuki T, Kazumori H, Takazawa S, Okamoto H, Kadowaki Y, Kinoshita Y.  Interleukin-8 regulates expression of Reg protein in Helicobacter pylori-infected gastric mucosa.  Am J Gastroenterol  2005  100(10):2157-66  PubMed ID: 16181363   DOI: 10.1111/j.1572-0241.2005.41915.x
5938  Kishimoto M, Okimura Y, Nakata H, Kudo T, Iguchi G, Takahashi Y, Kaji H, Chihara K.  Cloning and characterization of the 5(')-flanking region of the human ghrelin gene.  Biochem Biophys Res Commun  2003  305(1):186-92  PubMed ID: 12732215   DOI: 10.1016/s0006-291x(03)00722-8

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