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Cell No. : Cell Name
RCB0982 : ECC4  update : 2021/06/14
CommentHuman cell line derived from rectum cancer (small cell cancer). Expressing creatinine kinase of brain isoenzyme etc. ( PubMed ID: 8392984).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Motoyama, Teiichi
Originator Ishihara, Noriko
Year of deposit 1994
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 45 years
Tissue rectum
Disease name small-cell gastrointestinal
Metastastatic ability Yes
Classification cancer
History originator
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1190
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 59-63(50) : 59(1),60(4),61(10),62(34),63(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 4


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Reference
11693  T Fujiwara, T Motoyama, N Ishihara, H Watanabe, T Kumanishi, K Kato, H Ichinose, T Nagatsu  Characterization of four new cell lines derived from small-cell gastrointestinal carcinoma  Int J Cancer  1993  54(6):965-71  PubMed ID: 8392984

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User's Publication
11320  Hayashi I, Kanda S, Lamaningao P, Mishima N, Nishiyama T.  Shared expression of mucin12 in Ascaris lumbricoides and the human small intestine.  Mol. Biochem. Parasitol.  2019    PubMed ID: 30481538
11381  Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.  Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.  Chem. Biodivers.  2019    PubMed ID: 30645035
6405  Matsuo K, Taniguchi K, Hamamoto H, Ito Y, Futaki S, Inomata Y, Shima T, Asakuma M, Lee SW, Tanaka K, Okuda J, Kondo Y, Uchiyama K.  Delta-like 3 localizes to neuroendocrine cells and plays a pivotal role in gastrointestinal neuroendocrine malignancy.  Cancer Sci  2019  110(10):3122-3131  PubMed ID: 31369178
6406  Park Y, Kubo A, Komiya T, Coxon A, Beebe K, Neckers L, Meltzer PS, Kaye FJ.  Low-penetrant RB allele in small-cell cancer shows geldanamycin instability and discordant expression with mutant ras.  Cell Cycle  2008  7(15):2384-91  PubMed ID: 18677112



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