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Cell No. : Cell Name
RCB0978 : HIRS-BM  update : 2022/03/25
CommentHuman cell line derived from malignant mixed mesodermal tumor found in uterus (malignant mixed Müllerian tumor). Established from metastasis in bone marrow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ono, I. & Ishiwata, I. & Ishikawa, H.
Year of deposit 1994
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 16 years
Tissue muscle, bone marrow meta
Disease name mixed mesodermal tumor
Metastatic ability Yes
Metastatic tissue whole body
Classification cancer
Year of origin 1981
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_E060
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/3-4 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 41-51(50) : /45(1),46(4),47(1),48(35),49(3),51(1)
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
1838  Ono I, Ishiwata I, Tashiro A, Mihara A, Nakaguchi T, Ito S, Tokita N, Nakajima S, Ishiwata C, Soma M, et al.  Establishment and characterization of two human mixed mesodermal tumor cell lines from the same patient.  J Natl Cancer Inst  1984  72:1241-52  PubMed ID: 6328089  

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