Cell No. : Cell Name
update : 2024/01/17
Comment Producing IgG MoAb against human 29 kDa lectin detectable in many human tumors. Also see HH17-4.
Comment from the depositor
Terms and conditions A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Hisamitsu Pharmaceutical Co., Inc.
Order Form
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see ). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp ).
Basic information
Depositor
Sato, Shuji
Originator
Kusunoki, Takuma
Year of deposit
1993
Animal
_mouse x mouse
< Hybrid
Tissue
hybridoma
Classification
hybrid cells
Lifespan
infinite
Morphology
lymphocyte-like
Cellosaurus(Expasy)
CVCL_U188
hybridoma
Fusion_cell_1 name
splenocyte
Fusion_cell_1 strain
mouse, BALB/c
Fusion_cell_2 name
P3X63Ag8U1
Fusion_cell_2 strain
mouse, BALB/c
Fusion method
PEG, HAT selection
Antibody name
HH13-1
Subclass
IgG
Immunogen
Human 29 kDa lectin
Antigen_name
Human 29 kDa lectin
Antigen_m.w.
29000
Antigen_character
Human 29 kDa lectin
Antigen_epitope
Unknown
Antigen_positive
Many type of human tumors
deposit info lot info
Medium
Medium List
Culture type
Suspension cells
Culture method
浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium
RPMI1640 + 10% FBS
Antibiotics
Free
Passage method
dilution
Culture information
Passage ratio
1 : 4-10 split
SC frequency
Subculture : 2 times/week
Temperature
37
℃
CO2 concentration
5
%
Freeze medium
Medium + 10% DMSO
Freezing method
Slow freezing
Mycoplasma/Acholeplasma
(-)
Antibody subclass
IgG
Reference information
Reference
0
User's Publication
0
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