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Cell No. : Cell Name
RCB0959 : HH13-1  update : 2022/03/25
CommentProducing IgG MoAb against human 29 kDa lectin detectable in many human tumors. Also see HH17-4.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Hisamitsu Pharmaceutical Co., Inc.

1-25-11, Kannondai, Tsukuba-Shi, Ibaraki-Ken, Japan
Dr.SATO Shuji
Fax. +81-29-837-0685
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sato, Shuji
Originator Kusunoki, Takuma
Year of deposit 1993
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U188
hybridoma Fusion_cell_1 name splenocyte
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3X63Ag8U1
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name HH13-1
Subclass IgG
Immunogen Human 29 kDa lectin
Antigen_name Human 29 kDa lectin
Antigen_m.w. 29000
Antigen_character Human 29 kDa lectin
Antigen_epitope Unknown
Antigen_positive Many type of human tumors
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG
Reference information Reference 0
User's Publication 0


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