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Cell No. : Cell Name
RCB0881 : OCUB-M  update : 2021/02/26
CommentSame as OCUB-1, OCUB-F.
Comment from the depositorChromosome = pseudotetraploid.
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sawada, Tetsuji
Originator Sawada, Tetsuji
Year of deposit 1993
Original cell OCUB-1
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 53 years
Tissue mammary gland, pleural fluid
Disease name mammary tumor
Metastastatic ability Yes
Metastatsd tissue chest wall, pleura
Classification cancer
Year of origin 1988
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS + 0.5mM Sodium Pyruvate
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 62-156(50) : /74(5),75(5),76(7),77(5),78(3),79(9)/
deposit info
lot info
Reference information Reference 2
User's Publication 7

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5477  Ikeda K, Yashiro M, Sawada T, Hato F, Hasuma T, Ishikawa T, Hirakawa-YSChung K.  M(R) 77 KDA factor derived from fibroblasts stimulates the invasion ability of breast-cancer cells.  Int J Cancer  2001  92(2):181-6  PubMed ID: 11291043
1881  Sawada T, Chung, Y S, Nakata B, Kubo T, Kondo Y, Sogabe T, Onoda N, Ogawa Y, Yamada N, Sowa M  [Establishment and characterization of a human breast cancer cell line, OCUB-1]  Hum Cell  1994  7:138-44  PubMed ID: 7873497

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User's Publication
12142  Kazuyuki Kuramoto, Hiroyoshi Yamada, Takashi Shin, Yuki Sawada, Hidenori Azami, Tomohiro Yamada, Takeyuki Nagashima, Kei Ohnuki  Development of a potent and orally active activator of adenosine monophosphate-activated protein kinase (AMPK), ASP4132, as a clinical candidate for the treatment of human cancer  Bioorganic & Medicinal Chemistry  2020    PubMed ID: 32007387
11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826
11545  Uhr K, Prager-van der Smissen WJC, Heine AAJ, Ozturk B, van Jaarsveld MTM, Boersma AWM, Jager A, Wiemer EAC, Smid M, Foekens JA, Martens JWM.  MicroRNAs as possible indicators of drug sensitivity in breast cancer cell lines.  PLoS ONE  2019    PubMed ID: 31063487
3352  Sugimoto, Mayumi, Fujikawa, Akira, Womack, James E, Sugimoto, Yoshikazu  Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance.  Proc Natl Acad Sci U S A  2006  103:6454-9  PubMed ID: 16611727
3796  Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.  Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.  Int J Cancer  2003  107(1):53-9  PubMed ID: 12925956
5547  Orui H, Imaizumi S, Ogino T, Motoyama T.  Effects of bone morphogenetic protein-2 on human tumor cell growth and differentiation: a preliminary report.  J Orthop Sci  2000  5(6):600-4  PubMed ID: 11180925
13694  Kawamoto M, Shichijo S, Imai Y, Imaizumi T, Koga T, Yanaga H, Itoh K.  Expression of the SART-1 tumor rejection antigen in breast cancer  Int J Cancer  1999  80(1):64-7  PubMed ID: 9935232

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