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Cell No. : Cell Name
RCB0872 : LLC-GA5-CoL300  update : 2023/04/12
CommentPig kidney (LLC-PK1) cells expressing human P-glycoproteins.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Research Gate Building T2-A,
3-25-10 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821 Japan
Keio University Tonomachi Town Campus
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ueda, K. & Tanigawara, Y.
Year of deposit 1993
Original cell LLC-PK1
Animal _pig < Mammals
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene human P-glycoprotein
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U208
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium M199 + 10% FBS + 300ng/ml Colchicine
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 15

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4897  Saeki T1, Ueda K, Tanigawara Y, Hori R, Komano T.  Human P-glycoprotein transports cyclosporin A and FK506.  J Biol Chem  1993  268(9):6077-80  PubMed ID: 7681059  
4898  Saeki T, Ueda K, Tanigawara Y, Hori R, Komano T.  P-glycoprotein-mediated transcellular transport of MDR-reversing agents.  FEBS Lett  1993  324(1):99-102  PubMed ID: 8099333   DOI: 10.1016/0014-5793(93)81540-g
1888  Ueda K, Okamura N, Hirai M, Tanigawara Y, Saeki T, Kioka N, Komano T, Hori R  Human P-glycoprotein transports cortisol, aldosterone, and dexamethasone, but not progesterone.  J Biol Chem  1992  267:24248-52  PubMed ID: 1360010  

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User's Publication
21494  Han H, Akiyoshi T, Morita T, Tsuchitani T, Nabeta M, Yajima K, Imaoka A, Ohtani H.  The Effects of Jabara Juice on the Intestinal Permeation of Fexofenadine.  Biol Pharm Bull  2023  46(12):1745-1752  PubMed ID: 38044133   DOI: 10.1248/bpb.b23-00479
17713  Tajima K, Akanuma SI, Ohishi Y, Yoshida Y, Bauer B, Kubo Y, Inouye M, Hosoya KI.  Freshly isolated retinal capillaries to determine efflux transporter function at the inner BRB  J Control Release  2022  343:434-442  PubMed ID: 35104569   DOI: 10.1016/j.jconrel.2022.01.037
12143  May Phyu Thein Maw, Panadda Phattanawasin, Chanokporn Sukonpan and Nusara Piyapolrungroj  Possible Intestinal Absorption Enhancers from Citrus hystrix  E3S Web of Conferences  2020      DOI: 10.1051/e3sconf/202014102003
13493  Nusara Piyapolrungroj, Panadda Phattanawasin, Uthai Sotanaphun, May Phyu Thein Maw  Role of Citrus Limonoid as a Possible Bioavailability Enhancer  Key Engineering Materials  2020  859:132-138    DOI: 10.4028/
11690  May Phyu Thein Maw, Panadda Phattanawasin, Uthai Sotanaphun, Nusara Piyapolrungroj  Natural Furanocoumarin as Potential Oral Absorption Enhancer  Key Engineering Materials  2019      DOI: 10.4028/
10873    Effect of acidless orange on P-glycoprotein function   Thai Journal of Pharmaceutical Sciences  2016  40:139-142   
15682  K. Koga, M. Yoshinaga, Y. Uematsu, Y. Nagai, N. Miyakoshi, Y. Shimoda, M. Fujinaga, T. Minamimoto, M.-R. Zhang, M. Higuchi, N. Ohtake, T. Suhara, S. Chaki  TASP0434299: A Novel Pyridopyrimidin-4-One Derivative as a Radioligand for Vasopressin V1B Receptor  Journal of Pharmacology and Experimental Therapeutics  2016  357:495-508  PubMed ID: 27029585   DOI: 10.1124/jpet.116.232942
3830  Ohura K, Nakada Y, Kotani S, Imai T.  Design of Fexofenadine Prodrugs Based on Tissue-Specific Esterase Activity and Their Dissimilar Recognition by P-Glycoprotein.  J Pharm Sci.   2015  104(9):3076-83  PubMed ID: 25953731   DOI: 10.1002/jps.24467
8458  Ampasavate C, Sotanaphun U, Phattanawasin P, Piyapolrungroj N.  Effects of Curcuma spp. on P-glycoprotein function.  Phytomedicine  2010  17:506-12  PubMed ID: 19879740   DOI: 10.1016/j.phymed.2009.09.004
3432  Nakamura, Masato, Kawakita, Yasunori, Yasuhara, Akito, Fukasawa, Yoshiki, Yoshida, Koji, Sakagami, Kazunari, Nakazato, Atsuro  In vitro and in vivo evaluation of the metabolism and bioavailability of ester prodrugs of mgs0039 (3-(3,4-dichlorobenzyloxy)-2-amino-6-fluorobicyclo[3.1.0]hexane-2,6-dicarb oxylic Acid), a potent metabotropic glutamate receptor antagonist.  Drug Metab Dispos  2006  34:369-74  PubMed ID: 16326817   DOI: 10.1124/dmd.105.006213
4917  Yamamoto C, Murakami H, Koyabu N, Takanaga H, Matsuo H, Uchiumi T, Kuwano M, Naito M, Tsuruo T, Ohtani H, Sawada Y.  Contribution of P-glycoprotein to efflux of ramosetron, a 5-HT3 receptor antagonist, across the blood-brain barrier.  J Pharm Pharmacol  2002  54(8):1055-63  PubMed ID: 12195819   DOI: 10.1211/002235702320266208
4915  Soares-Da-Silva P1, Serrão MP.  Outward transfer of dopamine precursor L-3,4-dihydroxyphenylalanine (L-dopa) by native and human P-glycoprotein in LLC-PK(1) and LLC-GA5 col300 renal cells.  J Pharmacol Exp Ther  2000  293(2):697-704  PubMed ID: 10773046  
4918  Ohnishi A, Matsuo H, Yamada S, Takanaga H, Morimoto S, Shoyama Y, Ohtani H, Sawada Y.  Effect of furanocoumarin derivatives in grapefruit juice on the uptake of vinblastine by Caco-2 cells and on the activity of cytochrome P450 3A4.  Br J Pharmacol  2000  130(6):1369-77  PubMed ID: 10903978   DOI: 10.1038/sj.bjp.0703433
4916  Sampaio-Maia B, Gomes R, Soares-da-Silva P.  P-glycoprotein phosphorylation/dephosphorylation and cellular accumulation of L-DOPA in LLC-GA5 Col300 cells.  J Auton Pharmacol  1999  19(3):173-9  PubMed ID: 10511474   DOI: 10.1046/j.1365-2680.1999.00131.x
4923  Matsuzaki J, Yamamoto C, Miyama T, Takanaga H, Matsuo H, Ishizuka H, Kawahara Y, Kuwano M, Naito M, Tsuruo T, Sawada Y.  Contribution of P-glycoprotein to bunitrolol efflux across blood-brain barrier.  Biopharm Drug Dispos  1999  20(2):85-90  PubMed ID: 10206323   DOI: 10.1002/(sici)1099-081x(199903)20:2<85::aid-bdd159>;2-3

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