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Cell No. : Cell Name
RCB0871 : LLC-GA5-CoL150  update : 2025/06/06
CommentPig kidney (LLC-PK1) cells expressing human P-glycoproteins.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Research Gate Building T2-A,
3-25-10 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821 Japan
Keio University Tonomachi Town Campus
E-mail. tanigawara@keio.jp
Dr.TANIGAWARA Yusuke
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ueda, K. & Tanigawara, Y.
Year of deposit 1993
Original cell LLC-PK1
Animal _pig < Mammals
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene human P-glycoprotein
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U207
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium M199 + 10% FBS + 150ng/ml Colchicine
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
DNA barcoding OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 5
User's Publication 41


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Reference
4896  Kusunoki N, Takara K, Tanigawara Y, Yamauchi A, Ueda K, Komada F, Ku Y, Kuroda Y, Saitoh Y, Okumura K.  Inhibitory effects of a cyclosporin derivative, SDZ PSC 833, on transport of doxorubicin and vinblastine via human P-glycoprotein.  Jpn J Cancer Res  1998  89(11):1220-8  PubMed ID: 9914792   DOI: 10.1111/j.1349-7006.1998.tb00518.x
4895  Tanaka K, Hirai M, Tanigawara Y, Ueda K, Takano M, Hori R, Inui K.  Relationship between expression level of P-glycoprotein and daunorubicin transport in LLC-PK1 cells transfected with human MDR1 gene.  Biochem Pharmacol  1997  53(5):741-6  PubMed ID: 9113094   DOI: 10.1016/s0006-2952(96)00810-6
4894  NCBINCBI Logo Skip to main content Skip to navigation Resources How To About NCBI Accesskeys PubMed US National Library of Medicine National Institutes of Health Search databaseSearch termSearch AdvancedHelp Result Filters Format: AbstractSend to Pharm Res. 1996 Jul;13(7):1073-7. Effect of cyclosporin analogues and FK506 on transcellular transport of daunorubicin and vinblastine via P-glycoprotein. Tanaka K1, Hirai M, Tanigawara Y, Yasuhara M, Hori R, Ueda K, Inui K.  Effect of cyclosporin analogues and FK506 on transcellular transport of daunorubicin and vinblastine via P-glycoprotein.  Pharm Res  1996  13(7):1073-7  PubMed ID: 8842048   DOI: 10.1023/a:1016019010339
4893  Hirai M, Tanaka K, Shimizu T, Tanigawara Y, Yasuhara M, Hori R, Kakehi Y, Yoshida O, Ueda K, Komano T, et al.  Cepharanthin, a multidrug resistant modifier, is a substrate for P-glycoprotein.  J Pharmacol Exp Ther  1995  275(1):73-8  PubMed ID: 7562598  
1887  Tanigawara Y, Okamura N, Hirai M, Yasuhara M, Ueda K, Kioka N, Komano T, Hori R  Transport of digoxin by human P-glycoprotein expressed in a porcine kidney epithelial cell line (LLC-PK1).  J Pharmacol Exp Ther  1992  263:840-5  PubMed ID: 1359120  

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User's Publication
22442  Sunakawa H, Mizoi K, Takahashi R, Takahashi S, Ogihara T.  Impact of P-Glycoprotein-Mediated Drug-Endogenous Substrate Interactions on Androgen and Blood-Brain Barrier Permeability  J Pharm Sci  2024  113(1):228-234  PubMed ID: 37898165   DOI: 10.1016/j.xphs.2023.10.034
20055  Watanabe R, Esaki T, Ohashi R, Kuroda M, Kawashima H, Komura H, Natsume-Kitatani Y, Mizuguchi K.  Development of an In Silico Prediction Model for P-glycoprotein Efflux Potential in Brain Capillary Endothelial Cells toward the Prediction of Brain Penetration.  J Med Chem  2021    PubMed ID: 33619967   DOI: 10.1021/acs.jmedchem.0c02011
20180  Sasahara K, Shibata M, Sasabe H, Suzuki T, Takeuchi K, Umehara K, Kashiyama E.  Feature importance of machine learning prediction models shows structurally active part and important physicochemical features in drug design.  Drug Metab Pharmacokinet  2021  39:100401  PubMed ID: 34089983   DOI: 10.1016/j.dmpk.2021.100401
13493  Nusara Piyapolrungroj, Panadda Phattanawasin, Uthai Sotanaphun, May Phyu Thein Maw  Role of Citrus Limonoid as a Possible Bioavailability Enhancer  Key Engineering Materials  2020  859:132-138    DOI: 10.4028/www.scientific.net/KEM.859.132
13520  Morishita H, Okawa K, Ishii M, Mizoi K, Ito MA, Arakawa H, Yano K, Ogihara T.  Gastrointestinal absorption of pimozide is enhanced by inhibition of P-glycoprotein  PLoS One  2020  15(10):e0232438  PubMed ID: 33119612   DOI: 10.1371/journal.pone.0232438
11342  Washio I, Nakanishi T, Ishiguro N, Bister B, Tamai I.  Effect of endogenous multidrug resistance 1 and P-glycoprotein expression on anticancer drug resistance in colon cancer cell lines.  Biopharm Drug Dispos  2019    PubMed ID: 30556139   DOI: 10.1002/bdd.2167
4060  Miyamoto R, Nozawa T, Shiozuka K, Tabata K.   The Impact of Endogenous Breast Cancer Resistance Protein on Human P-Glycoprotein-Mediated Transport Assays Using LLC-PK1 Cells Transfected With Human P-Glycoprotein.  J Pharm Sci   2019  108:1085-1089  PubMed ID: 30339864   DOI: 10.1016/j.xphs.2018.10.012
11502  Ohashi R, Watanabe R, Esaki T, Taniguchi T, Torimoto-Katori N, Watanabe T, Ogasawara Y, Takahashi T, Tsukimoto M, Mizuguchi K.  Development of Simplified in Vitro P-Glycoprotein Substrate Assay and in Silico Prediction Models To Evaluate Transport Potential of P-Glycoprotein.  Mol. Pharm.  2019    PubMed ID: 30933526   DOI: 10.1021/acs.molpharmaceut.8b01143
11690  May Phyu Thein Maw, Panadda Phattanawasin, Uthai Sotanaphun, Nusara Piyapolrungroj  Natural Furanocoumarin as Potential Oral Absorption Enhancer  Key Engineering Materials  2019      DOI: 10.4028/www.scientific.net/KEM.819.63
11824  Yano K, Seto S, Kamioka H, Mizoi K, Ogihara T.  Testosterone and androstenedione are endogenous substrates of P-glycoprotein.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31585733   DOI: 10.1016/j.bbrc.2019.09.067
10564  Watanabe N, Higashi H, Nakamura S, Nomura K, Adachi Y, Taguchi M.  The possible clinical impact of risperidone on P-glycoprotein-mediated transport of tacrolimus: A case report and in vitro study.  Biopharm Drug Dispos  2018  39:30-37  PubMed ID: 29055041   DOI: 10.1002/bdd.2109
11121  Tomono T, Machida T, Kamioka H, Shibasaki Y, Yano K, Ogihara T.  Entinostat reverses P-glycoprotein activation in snail-overexpressing adenocarcinoma HCC827 cells.  PLoS ONE  2018  13(7):e0200015  PubMed ID: 29979729   DOI: 10.1371/journal.pone.0200015
10318  Kanamitsu K, Nozaki Y, Nagaya Y, Sugiyama Y, Kusuhara H.  Corrigendum to "Quantitative prediction of histamine H1 receptor occupancy by the sedative and non-sedative antagonists in the human central nervous system based on systemic exposure and preclinical data" [Drug Metab Pharmacokinet 32 (2017) 135-144].  Drug Metab. Pharmacokinet.  2017  32:228  PubMed ID: 28587746   DOI: 10.1016/j.dmpk.2017.05.003
10453  Higashi H, Watanabe N, Tamura R, Taguchi M.  In Vitro P-Glycoprotein-Mediated Transport of Tadalafil: A Comparison with Sildenafil.  Biol. Pharm. Bull.  2017  40:1314-1319  PubMed ID: 28769012   DOI: 10.1248/bpb.b17-00278
9614  Miyata K, Nakagawa Y, Kimura Y, Ueda K, Akamatsu M.  In vitro and in vivo evaluations of the P-glycoprotein-mediated efflux of dibenzoylhydrazines.  Toxicol. Appl. Pharmacol.  2016  298:40-7  PubMed ID: 26995013   DOI: 10.1016/j.taap.2016.03.008
9697  Miyata K, Nakagawa Y, Kimura Y, Ueda K, Akamatsu M.  Structure-activity relationships of dibenzoylhydrazines for the inhibition of P-glycoprotein-mediated quinidine transport.  Bioorg. Med. Chem.  2016  24:3184-91  PubMed ID: 27262425   DOI: 10.1016/j.bmc.2016.05.039
7397  Singh R, Rachumallu R, Bhateria M, Panduri J, Bhatta RS.  In vitro effects of standardized extract of Bacopa monniera and its five individual active constituents on human P-glycoprotein activity.  Xenobiotica  2015  45:741-9  PubMed ID: 25869246   DOI: 10.3109/00498254.2015.1017752
7432  Miyamoto R, Nozawa T, Kimura M, Shiozuka K, Tabata K.  Development and validation of semiautomated 96-well transport assay using LLC-PK1 cells transfected with human P-glycoprotein for high-throughput screening.  Assay Drug Dev Technol  2015  13:79-87  PubMed ID: 25785771   DOI: 10.1089/adt.2014.621
4909  Yano K, Mita S, Morimoto K, Haraguchi T, Arakawa H, Yoshida M, Yamashita F, Uchida T, Ogihara T.  Multiple Linear Regression Analysis Indicates Association of P-Glycoprotein Substrate or Inhibitor Character with Bitterness Intensity, Measured with a Sensor.  J Pharm Sci  2015  104(9):2789-94  PubMed ID: 25545612   DOI: 10.1002/jps.24232
4905  Yano K, Takimoto S, Motegi T, Tomono T, Hagiwara M, Idota Y, Morimoto K, Takahara A, Ogihara T.  Role of P-glycoprotein in regulating cilnidipine distribution to intact and ischemic brain.  Drug Metab Pharmacokinet  2014  29(3):254-8  PubMed ID: 24366438   DOI: 10.2133/dmpk.dmpk-13-rg-072
4907  Horie A, Ishida K, Shibata K, Taguchi M, Ozawa A, Hirono K, Ichida F, Hashimoto Y.  Pharmacokinetic variability of flecainide in younger Japanese patients and mechanisms for renal excretion and intestinal absorption.  Biopharm Drug Dispos  2014  35(3):145-53  PubMed ID: 24166085   DOI: 10.1002/bdd.1877
4912  Yokooji T, Kameda Y, Utsumi M, Mori N, Murakami T.  Interaction of hydrophobic components in female urine before and after childbirth with P-glycoprotein in vitro.  Pharmazie  2014  69(6):430-6  PubMed ID: 24974576  
19291  Ishiguro N, Shimizu H, Kishimoto W, Ebner T, Schaefer O.  Evaluation and prediction of potential drug-drug interactions of linagliptin using in vitro cell culture methods  Drug Metab Dispos  2013  41(1):149-58  PubMed ID: 23073734   DOI: 10.1124/dmd.112.048470
16161  Narumi Sugihara, Yuusuke Tsutsui, Tomohiko Tagashira, Tominari Choshi, Satoshi Hibino, Jun Kamishikiryou, Koji Furuno  The ability of gallate and pyrogallol moieties of catechins to inhibit P-glycoprotein function  Journal of Functional Foods  2011  3:298-304    DOI: 10.1016/j.jff.2011.05.005
19374    Optimization of Experimental Conditions for In vitro P-glycoprotein Assay Using LLC-GA5 Cells  Journal of Pharmaceutical Investigation  2010  40:363-366    DOI: 10.4333/kps.2010.40.6.363
18689  Morimoto K, Nakakariya M, Shirasaka Y, Kakinuma C, Fujita T, Tamai I, Ogihara T.  Oseltamivir (Tamiflu) efflux transport at the blood-brain barrier via P-glycoprotein  Drug Metab Dispos  2008  36(1):6-9  PubMed ID: 17940134   DOI: 10.1124/dmd.107.017699
4899  Tahara K, Kagawa Y, Takaai M, Taguchi M, Hashimoto Y.  Directional transcellular transport of bisoprolol in P-glycoprotein-expressed LLC-GA5-COL150 cells, but not in renal epithelial LLC-PK1 Cells.  Drug Metab Pharmacokinet  2008  23(5):340-6  PubMed ID: 18974611   DOI: 10.2133/dmpk.23.340
12809  Patanasethanont D, Nagai J, Yumoto R, Murakami T, Sutthanut K, Sripanidkulchai BO, Yenjai C, Takano M.  Effects of Kaempferia parviflora extracts and their flavone constituents on P-glycoprotein function  J Pharm Sci   2007  96(1):223-3  PubMed ID: 17031860   DOI: 10.1002/jps.20769
4903  Yoshida N, Koizumi M, Adachi I, Kawakami J.  Inhibition of P-glycoprotein-mediated transport by terpenoids contained in herbal medicines and natural products.  Food Chem Toxicol  2006  44(12):2033-9  PubMed ID: 16904803   DOI: 10.1016/j.fct.2006.07.003
3380  Tian, Run, Koyabu, Noriko, Morimoto, Satoshi, Shoyama, Yukihiro, Ohtani, Hisakazu, Sawada, Yasufumi  Functional induction and de-induction of P-glycoprotein by St. John's wort and its ingredients in a human colon adenocarcinoma cell line.  Drug Metab Dispos  2005  33:547-54  PubMed ID: 15640377   DOI: 10.1124/dmd.104.002485
4902  Yoshida N, Takagi A, Kitazawa H, Kawakami J, Adachi I.  Inhibition of P-glycoprotein-mediated transport by extracts of and monoterpenoids contained in Zanthoxyli fructus.  Toxicol Appl Pharmacol  2005  209(2):167-73  PubMed ID: 15890377   DOI: 10.1016/j.taap.2005.04.001
12836  Kimura O, Endo T, Hotta Y, Sakata M.  Effects of P-glycoprotein inhibitors on transepithelial transport of cadmium in cultured renal epithelial cells, LLC-PK1 and LLC-GA5-COL 150  Toxicology  2005  208(1):123-32  PubMed ID: 15664439   DOI: 10.1016/j.tox.2004.11.015
3216  Ishiguro, Naoki, Nozawa, Takashi, Tsujihata, Akiko, Saito, Asami, Kishimoto, Wataru, Yokoyama, Kazutoshi, Yotsumoto, Takafumi, Sakai, Kenji, Igarashi, Takashi, Tamai, Ikumi  Influx and efflux transport of H1-antagonist epinastine across the blood-brain barrier.  Drug Metab Dispos  2004  32:519-24  PubMed ID: 15100174   DOI: 10.1124/dmd.32.5.519
3217  Egashira, Kanoko, Ohtani, Hisakazu, Itoh, Suwako, Koyabu, Noriko, Tsujimoto, Masayuki, Murakami, Hideyasu, Sawada, Yasufumi  Inhibitory effects of pomelo on the metabolism of tacrolimus and the activities of CYP3A4 and P-glycoprotein.  Drug Metab Dispos  2004  32:828-33  PubMed ID: 15258108   DOI: 10.1124/dmd.32.8.828
4906  Honda Y, Ushigome F, Koyabu N, Morimoto S, Shoyama Y, Uchiumi T, Kuwano M, Ohtani H, Sawada Y.  Effects of grapefruit juice and orange juice components on P-glycoprotein- and MRP2-mediated drug efflux.  Br J Pharmacol  2004  143(7):856-64  PubMed ID: 15504753   DOI: 10.1038/sj.bjp.0706008
4911  Li Q, Sai Y, Kato Y, Muraoka H, Tamai I, Tsuji A.  Transporter-mediated renal handling of nafamostat mesilate.  J Pharm Sci  2004  93(2):262-72  PubMed ID: 14705184   DOI: 10.1002/jps.10534
4904  Toyobuku H, Tamai I, Ueno K, Tsuji A.  Limited influence of P-glycoprotein on small-intestinal absorption of cilostazol, a high absorptive permeability drug.  J Pharm Sci  2003  92(11):2249-59  PubMed ID: 14603510   DOI: 10.1002/jps.10490
2952  Murakami, Teruo, Fukuda, Takeshi, Yumoto, Ryoko, Nagai, Junya, Kuramoto, Taiju, Takano, Mikihisa  Interaction of endogenous compounds in human and rat urine with P-glycoprotein  Drug Metab Pharmacokinet  2002  17:245-252  PubMed ID: 15618676   DOI: 10.2133/dmpk.17.245
4900  Endo T, Kimura O, Sakata M.  Effects of P-glycoprotein inhibitors on cadmium accumulation in cultured renal epithelial cells, LLC-PK1, and OK.  Toxicol Appl Pharmacol  2002  185(3):166-71  PubMed ID: 12498733   DOI: 10.1006/taap.2002.9533
4901  Katoh M, Nakajima M, Yamazaki H, Yokoi T.  Inhibitory effects of CYP3A4 substrates and their metabolites on P-glycoprotein-mediated transport.  Eur J Pharm Sci  2001  12(4):505-13  PubMed ID: 11231118   DOI: 10.1016/s0928-0987(00)00215-3
3769  Naruhashi K, Tamai I, Inoue N, Muraoka H, Sai Y, Suzuki N, Tsuji A.  Active intestinal secretion of new quinolone antimicrobials and the partial contribution of P-glycoprotein.  J Pharm Pharmacol  2001  53(5):699-709  PubMed ID: 11370709   DOI: 10.1211/0022357011775820


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