Cell No. : Cell Name
RCB0870 : 12-1B6
update : 2024/01/17
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| Comment | Anti-human-myeloperoxidase MoAb producing. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Yamada, Michiyuki
|
| Originator |
Hur, S. -J. & Yamada, M.
|
| Year of deposit |
1993
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_J713
|
|
hybridoma
|
Fusion_cell_1 name |
spleen
|
| Fusion_cell_1 strain |
mouse, BALB/c
|
| Fusion_cell_2 name |
Sp2/0-Ag14
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
PEG #4000, HAT selection
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|
Antibody name |
12-1B6
|
| Subclass |
IgG2a
|
| Immunogen |
Human myeloperoxidase
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|
Antigen_name |
myeloperoxidase
|
| Antigen_m.w. |
150000
|
| Antigen_character |
Human origin native enzyme
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|
Antigen_positive |
Blood granulocyte, blood plasma, bone marrow
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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DMEM (low glucose) + 10% FBS (heat inactivated)
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| Antibiotics |
|
Free
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|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Isozyme |
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LD, NP
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| Antibody subclass |
|
IgG2a
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
1
|
| Reference |
|
1835
Yamada M, Hur, S J, Toda H
Isolation and characterization of extracellular myeloperoxidase precursor in HL-60 cell cultures.
Biochem Biophys Res Commun
1990
166:852-9
PubMed ID: 2154223
DOI: 10.1016/0006-291x(90)90888-t
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1836
Hur, S J, Toda, H K, Yamada M
Isolation and characterization of an unprocessed extracellular myeloperoxidase in HL-60 cell cultures.
J Biol Chem
1989
264:8542-8
PubMed ID: 2542279
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1834
Hur, S J, Yamada M
Production and characterization of two monoclonal antibodies against human myeloperoxidase.
Biotechnol Appl Biochem
1986
8:510-5
PubMed ID: 3028450
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