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Cell No. : Cell Name
RCB0870 : 12-1B6  update : 2022/03/24
CommentAnti-human-myeloperoxidase MoAb producing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamada, Michiyuki
Originator Hur, S. -J. & Yamada, M.
Year of deposit 1993
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J713
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name Sp2/0-Ag14
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name 12-1B6
Subclass IgG2a
Immunogen Human myeloperoxidase
Antigen_name myeloperoxidase
Antigen_m.w. 150000
Antigen_character Human origin native enzyme
Antigen_positive Blood granulocyte, blood plasma, bone marrow
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS (heat inactivated)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Antibody subclass IgG2a
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
1835  Yamada M, Hur, S J, Toda H  Isolation and characterization of extracellular myeloperoxidase precursor in HL-60 cell cultures.  Biochem Biophys Res Commun  1990  166:852-9  PubMed ID: 2154223  
1836  Hur, S J, Toda, H K, Yamada M  Isolation and characterization of an unprocessed extracellular myeloperoxidase in HL-60 cell cultures.  J Biol Chem  1989  264:8542-8  PubMed ID: 2542279  
1834  Hur, S J, Yamada M  Production and characterization of two monoclonal antibodies against human myeloperoxidase.  Biotechnol Appl Biochem  1986  8:510-5  PubMed ID: 3028450  

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User's Publication
8882  Munaka T, Abe H, Kanai M, Sakamoto T, Nakanishi H, Yamaoka T, Shoji S, Murakami A.  Real-time monitoring of antibody secretion from hybridomas on a microchip by time-resolved luminescence anisotropy analysis.  Anal. Biochem.  2006  353:1-6  PubMed ID: 16620756   DOI: 10.1016/j.ab.2006.03.009



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