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Cell No. : Cell Name
RCB0867 : 3-2H3  update : 2018/12/04
CommentAnti-human-myeloperoxidase MoAb producing.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Yamada, Michiyuki
Originator Hur, S. -J. & Yamada, M.
Year of deposit 1993
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name Sp2/0-Ag14
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name 3-2H3
Subclass IgG1
Immunogen Human myeloperoxidase
Antigen_name myeloperoxidase
Antigen_m.w. 150000
Antigen_character Human origin native enzyme
Antigen_positive Blood granulocyte, plasma, bone marrow cells
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS (heat inactivated)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Antibody subclass IgG1
Reference information Reference 3
User's Publication 4


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Reference
1835  Yamada M, Hur, S J, Toda H  Isolation and characterization of extracellular myeloperoxidase precursor in HL-60 cell cultures.  Biochem Biophys Res Commun  1990  166:852-9  PubMed ID: 2154223
1836  Hur, S J, Toda, H K, Yamada M  Isolation and characterization of an unprocessed extracellular myeloperoxidase in HL-60 cell cultures.  J Biol Chem  1989  264:8542-8  PubMed ID: 2542279
1834  Hur, S J, Yamada M  Production and characterization of two monoclonal antibodies against human myeloperoxidase.  Biotechnol Appl Biochem  1986  8:510-5  PubMed ID: 3028450

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User's Publication
9767  Hakoda M.  Development of dielectrophoresis separator with an insulating porous membrane using DC-Offset AC Electric Fields.  Biotechnol. Prog.  2016  32:1292-1300  PubMed ID: 27453404
8341  Hakoda M, Wakizaka Y, Hirota Y.  Separation of viable and nonviable animal cell using dielectrophoretic filter.  Biotechnol. Prog.  2010  26:1061-7  PubMed ID: 20205163
8471  Hirota Y, Hakoda M, Wakizaka Y.  Separation characteristics of animal cells using a dielectrophoretic filter.  Bioprocess Biosyst Eng  2009  33:607-12  PubMed ID: 19838737
8929  Hakoda M, Hachisu T, Wakizaka Y, Mii S, Kitajima N.  Development of a method to analyze single cell activity by using dielectrophoretic levitation.  Biotechnol. Prog.  2005  21:1748-53  PubMed ID: 16321061



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