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Cell No. : Cell Name
RCB0844 : 8C9  update : 2024/10/22
CommentProducing anti-human CD36 MoAb.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kudo, Eiji
Originator Kudo, Eiji
Year of deposit 1993
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J736
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name NS-1
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Subclass IgM
Immunogen human malignant fibrous histiocytoma
Antigen_name CD36
Antigen_m.w. 88kDa or 97kDa
Antigen_character platelet glycoprotein IV
Antigen_epitope cell surface molecule
Antigen_positive PBL, platelets, endothelial cells, adipocytes, monocyte
Antigen_negative erithroid, T cell, B cell, HUVECs
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium GIT + 5% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Antibody subclass IgM
Reference information Reference 1
User's Publication 0


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Reference
1934  Kudo E, Hirose T, Sano T, Hizawa K  Epitopic heterogeneity of the CD36 antigen expressed by normal and neoplastic endothelial cells. An immunohistochemical study with a novel monoclonal antibody 8C9.  Acta Pathol Jpn  1992  42:807-17  PubMed ID: 1281958   DOI: 10.1111/j.1440-1827.1992.tb01882.x

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