細胞番号 : 細胞名
RCB0820 : HKBML
update : 2024/03/08
|
細胞特性(Comment:英) | Human cell line derived from malignant lymphoma occurred in brain. Epstein-Barr virus positive. |
細胞特性(日) | ヒト脳内悪性リンパ腫由来細胞株。EBV陽性(PCR法にて確認済み)。 |
細胞特性(寄託者記述:英) | |
細胞特性(寄託者記述:日) | |
使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
|
|
寄託者 |
Ishiwata, Isamu
|
樹立者 |
Ishiwata, I. & Sato, E. & Ishikawa, H.
|
寄託日 |
1992
|
動物種 |
_human
< Mammals
|
属名 |
Homo
|
種名 |
sapiens
|
人種 |
Japanese
|
性別 |
Male
|
採取組織 |
brain
|
病名 |
Lymphoma
|
細胞分類 |
cancer
|
初代培養日 |
1991
|
|
細胞寿命 |
infinite
|
細胞形態 |
lymphocyte-like
|
Cellosaurus(Expasy) |
CVCL_8161
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Suspension cells
|
培養方法 |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
培地 |
|
HamF12 + 15% FBS
|
抗生物質 |
|
Free
|
|
継代方法 |
|
dilution
|
|
継代密度 |
|
1 : 4 split
|
継代・培地交換頻度 |
|
Subculture : 2 times/week
|
培養最適温度 |
|
37
℃
|
二酸化炭素濃度 |
|
5
%
|
凍結培地 |
|
Medium + 10% DMSO
|
凍結方法 |
|
Slow freezing
|
マイコプラズマ/アコレプラズマ |
|
(-)
|
ウィルス(HIV) |
|
(-)
|
ウィルス(HTLV-1) |
|
(-)
|
ウィルス(EBV) |
|
(+)
|
アイソザイム検査 |
|
LD, NP
|
染色体検査 |
|
36-96(50) : /45(1),46(2),47(5),48(37),49(1),96(1)
|
個体識別検査 |
|
OK
|
画像情報 |
寄託時情報 | ロット情報 |
|
|
文献情報 |
Reference(英) |
0件
|
Reference(日) |
0件
|
利用者成果(英) |
16件
|
利用者成果(日) |
0件
|
利用者成果(英) |
22337
Osako R, Hayano A, Kawaguchi A, Yamanaka R.
Single-cell RNA-seq reveals diverse molecular signatures associated with Methotrexate resistance in primary central nervous system lymphoma cells
J Neurooncol
2024
2024 Dec 5
PubMed ID: 39636551
DOI: 10.1007/s11060-024-04893-y
|
21284
Suzuki K, Yamamoto J, Toh K, Miyaoka R.
5‑aminiolevulinic acid induces a radiodynamic effect with enhanced delayed reactive oxygen species production under hypoxic conditions in lymphoma cells: An in vitro study.
Exp Ther Med
2023
26(1):360
PubMed ID: 37324514
DOI: 10.3892/etm.2023.12059
|
16945
Yano H, Fujiwara Y, Horlad H, Pan C, Kai K, Niino D, Ohsawa K, Higashi M, Nosaka K, Okuno Y, Tamaru JI, Mukasa A, Matsuoka M, Komohara Y.
Blocking cholesterol efflux mechanism is a potential target for antilymphoma therapy
Cancer Sci
2022
113(6):2129-2143
PubMed ID: 35343027
DOI: 10.1111/cas.15349
|
20208
Takashima Y, Kawaguchi A, Fukai J, Iwadate Y, Kajiwara K, Hondoh H, Yamanaka R.
Survival prediction based on the gene expression associated with cancer morphology and microenvironment in primary central nervous system lymphoma.
PLoS One
2021
16(6):e0251272
PubMed ID: 34166375
DOI: 10.1371/journal.pone.0251272
|
9864
Yasuo Takashima, Azusa Hayano, Ryuya Yamanaka
Metabolome Analysis Reveals Excessive Glycolysis via PI3K/AKT/mTOR and RAS/MAPK Signaling in Methotrexate-Resistant Primary CNS Lymphoma-Derived Cells
Clin Cancer Res
2020
26(11):2754-2766
PubMed ID: 32108030
DOI: 10.1158/1078-0432.CCR-18-3851
|
11997
Muta H, Sugita Y, Furuta T, Shiimura Y, Ohshima K, Nakashima K, Sato K, Morioka M, Abe H, Nozawa T, Fujii Y, Kakita A.
Expression of the ghrelin/growth hormone secretagogue receptor axis and its functional role in promoting tumor growth in primary central nervous system lymphomas.
Neuropathology
2020
PubMed ID: 31925841
DOI: 10.1111/neup.12634
|
13062
Takashima Y, Hamano M, Fukai J, Iwadate Y, Kajiwara K, Kobayashi T, Hondoh H, Yamanaka R.
GSEA-assisted gene signatures valid for combinations of prognostic markers in PCNSL.
Sci Rep
2020
PubMed ID: 32439996
DOI: 10.1038/s41598-020-65463-6
|
13099
Fujimoto K, Shinojima N, Hayashi M, Nakano T, Ichimura K, Mukasa A.
Histone deacetylase inhibition enhances the therapeutic effects of methotrexate on primary central nervous system lymphoma.
Neurooncol Adv
2020
PubMed ID: 32793886
DOI: 10.1093/noajnl/vdaa084
|
11518
Hayano A, Takashima Y, Yamanaka R.
Cell-type-specific sensitivity of bortezomib in the methotrexate-resistant primary central nervous system lymphoma cells.
Int. J. Clin. Oncol.
2019
PubMed ID: 30993483
DOI: 10.1007/s10147-019-01451-9
|
11849
Mulazzani M, Huber M, Borchard S, Langer S, Angele B, Schuh E, Meinl E, Dreyling M, Birnbaum T, Straube A, Koedel U, von Baumgarten L.
APRIL and BAFF: novel biomarkers for central nervous system lymphoma.
J Hematol Oncol
2019
PubMed ID: 31615554
DOI: 10.1186/s13045-019-0796-4
|
6897
Takashima Y, Yoshimura T, Kano Y, Hayano A, Hondoh H, Ikenaka K, Yamanaka R.
Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.
BMC Cancer
2019
19(1):910
PubMed ID: 31510952
DOI: 10.1186/s12885-019-6129-8
|
11125
Miyasato Y, Takashima Y, Takeya H, Yano H, Hayano A, Nakagawa T, Makino K, Takeya M, Yamanaka R, Komohara Y.
The expression of PD-1 ligands and IDO1 by macrophage/microglia in primary central nervous system lymphoma.
J Clin Exp Hematop
2018
58(2):95-101
PubMed ID: 29998979
DOI: 10.3960/jslrt.18001
|
11858
Shinojima N, Fujimoto K, Makino K, Todaka K, Yamada K, Mikami Y, Oda K, Nakamura K, Jono H, Kuratsu JI, Nakamura H, Yano S, Mukasa A.
Clinical significance of polyglutamylation in primary central nervous system lymphoma.
Acta Neuropathol Commun
2018
PubMed ID: 29475458
DOI: 10.1186/s40478-018-0522-4
|
10300
Takahashi Y, Sawada T, Akahane T, Kawase Y, Ikeda H, Makino K, Nakamura H, Hide T, Yano S, Hashimoto N, Kamada H.
Monocyte chemoattractant protein 1 expression and proliferation in primary central nervous system lymphoma.
Oncol Lett
2017
14:264-270
PubMed ID: 28693163
DOI: 10.3892/ol.2017.6122
|
14351
Ma C, Horlad H, Pan C, Yano H, Ohnishi K, Fujiwara Y, Matsuoka M, Lee A, Niidome T, Yamanaka R, Takeya M, Komohara Y.
Stat3 inhibitor abrogates the expression of PD-1 ligands on lymphoma cell lines
J Clin Exp Hematop
2017
57(1):21-25
PubMed ID: 28496056
DOI: 10.3960/jslrt.17006
|
7310
Mizowaki T, Sasayama T, Tanaka K, Mizukawa K, Takata K, Nakamizo S, Tanaka H, Nagashima H, Nishihara M, Hirose T, Itoh T, Kohmura E.
STAT3 activation is associated with cerebrospinal fluid interleukin-10 (IL-10) in primary central nervous system diffuse large B cell lymphoma.
J. Neurooncol.
2015
124:165-74
PubMed ID: 26080800
DOI: 10.1007/s11060-015-1843-9
|