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Cell No. : Cell Name
RCB0808 : MMAc  update : 2022/08/12
CommentHuman melanoma, spindle-shaped. Said DOPA (+).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Katayama, Hiroshi
Originator Katayama, Hiroshi
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 39 years
Tissue melanoma, ascites meta
Metastastatic ability Yes
Metastatsd tissue liver, lymph node et
Classification cancer
Year of origin 1992
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_5951
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, MPI, PepB
Chromosome mode 46-232(50) : /76(7),77(7),78(3),79(1),80(6),81(5)/
STR(human) OK
deposit info
lot info
Reference information Reference 0
User's Publication 11

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User's Publication
20799  Fujimoto M, Takii R, Matsumoto M, Okada M, Nakayama KI, Nakato R, Fujiki K, Shirahige K, Nakai A.  HSF1 phosphorylation establishes an active chromatin state via the TRRAP-TIP60 complex and promotes tumorigenesis.  Nat Commun  2022  13(1):4355  PubMed ID: 35906200   DOI: 10.1038/s41467-022-32034-4
12521  Hayashi Y, Jia W, Kidoya H, Muramatsu F, Tsukada Y, Takakura N.  Galectin-3 Inhibits Cancer Metastasis by Negatively Regulating Integrin β3 Expression  Am J Pathol  2019  189(4):900-910  PubMed ID: 30653955   DOI: 10.1016/j.ajpath.2018.12.005
7328  Kim HJ, Kim JS, Woo JT, Lee IS, Cha BY.  Hyperpigmentation mechanism of methyl 3,5-di-caffeoylquinate through activation of p38 and MITF induction of tyrosinase.  Acta Biochim. Biophys. Sin. (Shanghai)  2015  47:548-56  PubMed ID: 26018825   DOI: 10.1093/abbs/gmv040
7375  Kim HJ, Kim IS, Dong Y, Lee IS, Kim JS, Kim JS, Woo JT, Cha BY.  Melanogenesis-inducing effect of cirsimaritin through increases in microphthalmia-associated transcription factor and tyrosinase expression.  Int J Mol Sci  2015  16:8772-88  PubMed ID: 25903150   DOI: 10.3390/ijms16048772
3689  Nobeyama Y, Okochi-Takada E, Furuta J, Miyagi Y, Kikuchi K, Yamamoto A, Nakanishi Y, Nakagawa H, Ushijima T.  Silencing of tissue factor pathway inhibitor-2 gene in malignant melanomas.  Int J Cancer  2007  121(2):301-7  PubMed ID: 17372906   DOI: 10.1002/ijc.22637
3445  Furuta, Junichi, Nobeyama, Yoshimasa, Umebayashi, Yoshihiro, Otsuka, Fujio, Kikuchi, Kanako, Ushijima, Toshikazu  Silencing of Peroxiredoxin 2 and aberrant methylation of 33 CpG islands in putative promoter regions in human malignant melanomas.  Cancer Res  2006  66:6080-6  PubMed ID: 16778180   DOI: 10.1158/0008-5472.CAN-06-0157
3093  Furuta, Junichi, Kaneda, Atsushi, Umebayashi, Yoshihiro, Otsuka, Fujio, Sugimura, Takashi, Ushijima, Toshikazu  Silencing of the thrombomodulin gene in human malignant melanoma.  Melanoma Res  2005  15:15-20  PubMed ID: 15714116   DOI: 10.1097/00008390-200502000-00004
13482  Tsutsumida A, Hamada J, Tada M, Aoyama T, Furuuchi K, Kawai Y, Yamamoto Y, Sugihara T, Moriuchi T.  Epigenetic silencing of E- and P-cadherin gene expression in human melanoma cell lines  Int J Oncol  2004  25(5):1415-21  PubMed ID: 15492833  
15609  Furuta J, Umebayashi Y, Miyamoto K, Kikuchi K, Otsuka F, Sugimura T, Ushijima T.  Promoter methylation profiling of 30 genes in human malignant melanoma  Cancer Sci   2004  95(12):962-8  PubMed ID: 15596045   DOI: 10.1111/j.1349-7006.2004.tb03184.x
3688  Satomi H1, Wang B, Fujisawa H, Otsuka F.  Interferon-beta from melanoma cells suppresses the proliferations of melanoma cells in an autocrine manner.  Cytokine  2002  18(2):108-15  PubMed ID: 12096926   DOI: 10.1006/cyto.2002.1028
13483  Okubo Y, Hamada J, Takahashi Y, Tada M, Tsutsumida A, Furuuchi K, Aoyama T, Sugihara T, Moriuchi T.  Transduction of HOXD3-antisense into human melanoma cells results in decreased invasive and motile activities  Clin Exp Metastasis  2002  19(6):503-11  PubMed ID: 12405287   DOI: 10.1023/a:1020346211686

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