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Cell No. : Cell Name
RCB0808 : MMAc  update : 2020/09/24
CommentHuman melanoma, spindle-shaped. Said DOPA (+). Cell growth is slow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Katayama, Hiroshi
Originator Katayama, Hiroshi
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 39 years
Tissue melanoma, ascites meta
Metastastatic ability Yes
Metastatsd tissue liver, lymph node et
Classification cancer
Year of origin 1992
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP, MPI, PepB
Chromosome mode 46-232(50) : /76(7),77(7),78(3),79(1),80(6),81(5)/
Images
deposit info
lot info
Reference information Reference 0
User's Publication 5


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Reference

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User's Publication
7328  Kim HJ, Kim JS, Woo JT, Lee IS, Cha BY.  Hyperpigmentation mechanism of methyl 3,5-di-caffeoylquinate through activation of p38 and MITF induction of tyrosinase.  Acta Biochim. Biophys. Sin. (Shanghai)  2015  47:548-56  PubMed ID: 26018825
3689  Nobeyama Y, Okochi-Takada E, Furuta J, Miyagi Y, Kikuchi K, Yamamoto A, Nakanishi Y, Nakagawa H, Ushijima T.  Silencing of tissue factor pathway inhibitor-2 gene in malignant melanomas.  Int J Cancer  2007  121(2):301-7  PubMed ID: 17372906
3445  Furuta, Junichi, Nobeyama, Yoshimasa, Umebayashi, Yoshihiro, Otsuka, Fujio, Kikuchi, Kanako, Ushijima, Toshikazu  Silencing of Peroxiredoxin 2 and aberrant methylation of 33 CpG islands in putative promoter regions in human malignant melanomas.  Cancer Res  2006  66:6080-6  PubMed ID: 16778180
3093  Furuta, Junichi, Kaneda, Atsushi, Umebayashi, Yoshihiro, Otsuka, Fujio, Sugimura, Takashi, Ushijima, Toshikazu  Silencing of the thrombomodulin gene in human malignant melanoma.  Melanoma Res  2005  15:15-20  PubMed ID: 15714116
3688  Satomi H1, Wang B, Fujisawa H, Otsuka F.  Interferon-beta from melanoma cells suppresses the proliferations of melanoma cells in an autocrine manner.  Cytokine  2002  18(2):108-15  PubMed ID: 12096926



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