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Cell No. : Cell Name
RCB0806 : Jurkat  update : 2022/03/28
CommentHuman T cell line, the same patient as JM. IL-2 productivity of this line was undetermined.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Todokoro, Kazuo
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 years
Tissue blood
Disease name acute T cell leukemia
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0065
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode Lot.1 : 42-92(50) : 42(2),43(1),44(6),45(21),46(14),47(5)/
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
Jurkat_CD3.pdf
Reference information Reference 7
User's Publication 60


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Reference
1640  LaGree KA, Lee AT, Stetten G, Strauss PR.  The human Jurkat (FHCRC-11) cell line is heterogeneous in ploidy and cell size and releases detergent-soluble DNA.  Exp Hematol  1988  16(8):686-90  PubMed ID: 3165345
4749  Wendler I1, Jentsch KD, Schneider J, Hunsmann G.  Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells.  Med Microbiol Immunol  1987  176(5):273-80  PubMed ID: 2443825
1685  Weiss A, Wiskocil RL, Stobo JD.  The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level.  J Immunol  1984  133(1):123-8  PubMed ID: 6327821
1638  Pawelec G, Borowitz A, Krammer PH, Wernet P.  Constitutive interleukin 2 production by the JURKAT human leukemic T cell line.  Eur J Immunol  1982  12(5):387-92  PubMed ID: 6980126
1637  Kahle P, Wernet P, Rehbein A, Kumbier I, Pawelec G.  Cloning of functional human T lymphocytes by limiting dilution: impact of filler cells and interleukin 2 sources on cloning efficiencies.  Scand J Immunol  1981  14(5):493-502  PubMed ID: 6278580
515  Gillis S, Watson J.  Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.  J Exp Med  1980  152(6):1709-19  PubMed ID: 6778951
902  Schneider U, Schwenk HU, Bornkamm G.  Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.  Int J Cancer  1977  19(5):621-6  PubMed ID: 68013

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User's Publication
20498  Aoyama M, Tada M, Yokoo H, Demizu Y, Ishii-Watabe A.  Fcγ Receptor-Dependent Internalization and Off-Target Cytotoxicity of Antibody-Drug Conjugate Aggregates.  Pharm Res  2022    PubMed ID: 34961908
16069  Nonaka Y, Ogawa T, Shoji H, Nishi N, Kamitori S, Nakamura T.  Crystal structure and conformational stability of a galectin-1 tandem-repeat mutant with a short linker  Glycobiology  2022  cwab101  PubMed ID: 34735570
13052  Kawai S, Suzuki M, Arimoto S, Korenaga T, Yasukawa T.  Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation.  Analyst  2020    PubMed ID: 32462157
13322  Bakalova R, Semkova S, Ivanova D, Zhelev Z, Miller T, Takeshima T, Shibata S, Lazarova D, Aoki I, Higashi T.  Selective Targeting of Cancerous Mitochondria and Suppression of Tumor Growth Using Redox-Active Treatment Adjuvant.  Oxid Med Cell Longev  2020    PubMed ID: 33204397
10599  Yoshimi Utaka, Gengo Kashiwazaki, Sachiko Tajima, Yuko Fujiwara, Keitaro Sumi, Tomohiro Itoh, Takashi Kitayama  Antiproliferative effects of zerumbone-pendant derivatives on human T-cell lymphoid cell line Jurkat cells  Tetrahedron  2019  75:1343-1350 
10946  Tsubasa Koga, Keiko Morotomi-Yano, Takashi Sakugawa, Hisato Saitoh, Ken-Ichi Yano  Nanosecond pulsed electric fields induce extracellular release of chromosomal DNA and histone citrullination in neutrophil-differentiated HL-60 cells  Sci Rep  2019  9(1):8451  PubMed ID: 31186478
12485  Sharma N, Baek K, Shimokawa N, Takagi M.  Effect of temperature on raft-dependent endocytic cluster formation during activation of Jurkat T cells by concanavalin A  J Biosci Bioeng  2019  127(4):479-485  PubMed ID: 30355461
14826  Nakashima M, Yamochi T, Watanabe M, Uchimaru K, Utsunomiya A, Higashihara M, Watanabe T, Horie R.  CD30 Characterizes Polylobated Lymphocytes and Disease Progression in HTLV-1-Infected Individuals  Clin Cancer Res  2018  24(21):5445-5457  PubMed ID: 30068708
14450  Yabuuchi S, Endo S, Baek K, Hoshino K, Tsujino Y, Vestergaard MC, Takagi M.  Raft-dependent endocytic movement and intracellular cluster formation during T cell activation triggered by concanavalin A  J Biosci Bioeng  2017  124(6):685-693  PubMed ID: 28711300
7069  Kataoka K, Shiraishi Y, Takeda Y, Sakata S, Matsumoto M, Nagano S, Maeda T, Nagata Y, Kitanaka A, Mizuno S, Tanaka H, Chiba K, Ito S, Watatani Y, Kakiuchi N, Suzuki H, Yoshizato T, Yoshida K, Sanada M, Itonaga H, Imaizumi Y, Totoki Y, Munakata W, Nakamura H, Hama N, Shide K, Kubuki Y, Hidaka T, Kameda T, Masuda K, Minato N, Kashiwase K, Izutsu K, Takaori-Kondo A, Miyazaki Y, Takahashi S, Shibata T, Kawamoto H, Akatsuka Y, Shimoda K, Takeuchi K, Seya T, Miyano S, Ogawa S.  Aberrant PD-L1 expression through 3'-UTR disruption in multiple cancers.  Nature  2016  534:402-6  PubMed ID: 27281199
9159  Tsukasa Nakanishi, Yuan Song, Cuiying He 1, Duo Wang, Kentaro Morita, Junichi Tsukada, Tamotsu Kanazawa, Yasuhiro Yoshida  Autophagy Is Associated With Cucurbitacin D-induced Apoptosis in Human T Cell Leukemia Cells  Med Oncol  2016  33 (4), 30  PubMed ID: 26913856
12962  Hayashi K, Ouchi M, Endou H, Anzai N.  HOXB9 acts as a negative regulator of activated human T cells in response to amino acid deficiency  Immunol Cell Biol  2016  94(6):612-7.  PubMed ID: 26926958
14128  REGINA APPIAH-OPONG, ISAAC KWADWO ASANTE, DORCAS OSEI SAFO, ISAAC TUFFOUR, EBENEZER OFORI- ATTAH, TAKUHIRO UTO, ALEXANDER KWADWO NYARKO  Cytotoxic effects of Albizia zygia (DC) JF Macbr, a Ghanaian medicinal plant, against human T-lymphoblast-like leukemia, prostate and breast cancer cell lines  International Journal of Pharmacy and Pharmaceutical Sciences  2016  8 
7244  Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T.  Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS.  Nat Commun  2015  6:8021  PubMed ID: 26282514
7435  Ebina H, Kanemura Y, Misawa N, Sakuma T, Kobayashi T, Yamamoto T, Koyanagi Y.  A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector.  PLoS ONE  2015  10:e0120047  PubMed ID: 25781496
15173  Kataoka C, Suzuki T, Kotani O, Iwata-Yoshikawa N, Nagata N, Ami Y, Wakita T, Nishimura Y, Shimizu H.  The Role of VP1 Amino Acid Residue 145 of Enterovirus 71 in Viral Fitness and Pathogenesis in a Cynomolgus Monkey Model  PLoS Pathog  2015  11(7):e1005033  PubMed ID: 26181772
14392  Tada M, Ishii-Watabe A, Suzuki T, Kawasaki N.  Development of a cell-based assay measuring the activation of FcγRIIa for the characterization of therapeutic monoclonal antibodies  PLoS One  2014  9(4):e95787  PubMed ID: 24752341
7821  Qi H, Takano H, Kato Y, Wu Q, Ogata C, Zhu B, Murata Y, Nakamura Y.  Hydrogen [corrected] peroxide-dependent photocytotoxicity by phloxine B, a xanthene-type food colorant.  Biochim. Biophys. Acta  2011  1810:704-12  PubMed ID: 21565256
7874  Wang F, Fang W, Zhang MR, Zhao M, Liu B, Wang Z, Hua Z, Yang M, Kumata K, Hatori A, Yamasaki T, Yanamoto K, Suzuki K.  Evaluation of chemotherapy response in VX2 rabbit lung cancer with 18F-labeled C2A domain of synaptotagmin I.  J. Nucl. Med.  2011  52:592-9  PubMed ID: 21421722
6237  Tanaka N, Abe-Dohmae S, Iwamoto N, Fitzgerald ML, Yokoyama S.  HMG-CoA reductase inhibitors enhance phagocytosis by upregulating ATP-binding cassette transporter A7.  Atherosclerosis   2011  217(2):407-14  PubMed ID: 21762915
14408  Ding N, Yamashita U, Matsuoka H, Sugiura T, Tsukada J, Noguchi J, Yoshida Y.  Apoptosis induction through proteasome inhibitory activity of cucurbitacin D in human T-cell leukemia  Cancer  2011  117(12):2735-46  PubMed ID: 21656752
8582  Zhang G, Wu H, Zhu B, Shimoishi Y, Nakamura Y, Murata Y.  Induction of apoptosis by beta-carotene and dimethyl tetrasulfide assisted by UVA irradiation in HL-60 cells.  Biosci. Biotechnol. Biochem.  2009  73:1014-20  PubMed ID: 19420723
8667  Kobayashi K, Yasuda Y, Shintani Y, Sumitomo T, Saga T, Kimura M, Yamamoto A, Mori T, Maeda A, Yamaguchi Y, Morita A.  The development of a filter to enhance the efficacy and safety of excimer light (308 nm) therapy.  Photodermatol Photoimmunol Photomed  2009  25:30-6  PubMed ID: 19152513
9078  Yorihiro Nishimura, Masayuki Shimojima, Yoshio Tano, Tatsuo Miyamura, Takaji Wakita, Hiroyuki Shimizu  Human P-selectin Glycoprotein ligand-1 Is a Functional Receptor for Enterovirus 71  Nat Med  2009  15 (7), 794-7  PubMed ID: 19543284
14412  Takeshita A, Shinjo K, Yamakage N, Ono T, Hirano I, Matsui H, Shigeno K, Nakamura S, Tobita T, Maekawa M, Ohnishi K, Sugimoto Y, Kiyoi H, Naoe T, Ohno R.  CMC-544 (inotuzumab ozogamicin) shows less effect on multidrug resistant cells: analyses in cell lines and cells from patients with B-cell chronic lymphocytic leukaemia and lymphoma  Br J Haematol  2009  146(1):34-43  PubMed ID: 19388933
16556  Nakase I, Hirose H, Tanaka G, Tadokoro A, Kobayashi S, Takeuchi T, Futaki S.  Cell-surface accumulation of flock house virus-derived peptide leads to efficient internalization via macropinocytosis  Mol Ther  2009  17(11):1868-76  PubMed ID: 19707187
8761  Okamura A, Hagiwara T, Yamagami S, Yamaguchi M, Shinbo T, Kanamori T, Kondo S, Miwa K, Itagaki I.  Effective cell separation utilizing poly(N-isopropylacrylamide)-grafted polypropylene membrane containing adsorbed antibody.  J. Biosci. Bioeng.  2008  105:221-5  PubMed ID: 18397772
9059  Y-C Jung, E Mizuki, T Akao, J-C Côté  Isolation and Characterization of a Novel Bacillus Thuringiensis Strain Expressing a Novel Crystal Protein With Cytocidal Activity Against Human Cancer Cells  J Appl Microbiol  2007  103 (1), 65-79  PubMed ID: 17584453
15599  Kato N, Tanaka J, Sugita J, Toubai T, Miura Y, Ibata M, Syono Y, Ota S, Kondo T, Asaka M, Imamura M.  Regulation of the expression of MHC class I-related chain A, B (MICA, MICB) via chromatin remodeling and its impact on the susceptibility of leukemic cells to the cytotoxicity of NKG2D-expressing cells  Leukemia  2007  21(10):2103-8  PubMed ID: 17625602
16559  Nishida M, Usuda S, Okabe M, Miyakoda H, Komatsu M, Hanaoka H, Teshigawara K, Niwa O.  Characterization of novel murine anti-CD20 monoclonal antibodies and their comparison to 2B8 and c2B8 (rituximab)  Int J Oncol  2007  31(1):29-40  PubMed ID: 17549402
15553  Masatoshi Beppu, Yoshie Nakadai, Yu Igarashi  Apoptosis Inducing and Enhancing Activities of Environmental Estrogenic Compounds  Journal of Health Science  2006  52:831-837 
3413  Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72  PubMed ID: 16428294
2892  Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis  J Biochem (Tokyo)  2005  137:17-25  PubMed ID: 15713879
2974  Tamura H, Dan K, Tamada K, Nakamura K, Shioi Y, Hyodo H, Wang SD, Dong H, Chen L, Ogata K  Expression of functional B7-H2 and B7.2 costimulatory molecules and their prognostic implications in de novo acute myeloid leukemia  Clin Cancer Res  2005  15:5708-5717  PubMed ID: 16115907
2875  Miyoshi N, Naniwa K, Kumagai T, Uchida K, Osawa T, Nakamura Y.  Alpha-tocopherol-mediated caspase-3 up-regulation enhances susceptibility to apoptotic stimuli.  Biochem Biophys Res Commun  2005  334(2):466-73  PubMed ID: 16009347
2891  Kishimoto S, Muramatsu M, Gokoh M, Oka S, Waku K, Sugiura T.  Endogenous cannabinoid receptor ligand induces the migration of human natural killer cells.  J Biochem  2005  137(2):217-23  PubMed ID: 15749836
2896  Yamanaka M, Eda S, Beppu M.  Carbohydrate chains and phosphatidylserine successively work as signals for apoptotic cell removal.  Biochem Biophys Res Commun  2005  328(1):273-80  PubMed ID: 15670780
3349  Hirano K, Miki Y, Hirai Y, Sato R, Itoh T, Hayashi A, Yamanaka M, Eda S, Beppu M.  A multifunctional shuttling protein nucleolin is a macrophage receptor for apoptotic cells.  J Biol Chem  2005  280(47):39284-93  PubMed ID: 16135517
3387  Ogawa Y, Morikawa N, Ohkubo-Suzuki A, Miyoshi S, Arakawa H, Kita Y, Nishimura S.  An epoch-making application of discharge plasma phenomenon to gene-transfer.  Biotechnol Bioeng  2005  92(7):865-70  PubMed ID: 16170826
9048  Shiro Okumura, Hiroyuki Saitoh, Tomoyuki Ishikawa, Naoya Wasano, Satoko Yamashita, Ken-Ichi Kusumoto, Tetsuyuki Akao, Eiichi Mizuki, Michio Ohba, Kuniyo Inouye  Identification of a Novel Cytotoxic Protein, Cry45Aa, From Bacillus Thuringiensis A1470 and Its Selective Cytotoxic Activity Against Various Mammalian Cell Lines  J Agric Food Chem  2005  53 (16), 6313-8  PubMed ID: 16076112
4437  Hamasu T, Inanami O, Tsujitani M, Yokoyama K, Takahashi E, Kashiwakura I, Kuwabara M.  Post-irradiation hypoxic incubation of X-irradiated MOLT-4 cells reduces apoptotic cell death by changing the intracellular redox state and modulating SAPK/JNK pathways  Apoptosis  2005  10(3):557-67  PubMed ID: 15909118
16557  Matsura T, Togawa A, Kai M, Nishida T, Nakada J, Ishibe Y, Kojo S, Yamamoto Y, Yamada K.  The presence of oxidized phosphatidylserine on Fas-mediated apoptotic cell surface  Biochim Biophys Acta  2005  1736(3):181-8  PubMed ID: 16168707
16606  Hattori N, Ikekubo K, Nakaya Y, Kitagawa K, Inagaki C.  Immunoglobulin G subclasses and prolactin (PRL) isoforms in macroprolactinemia due to anti-PRL autoantibodies  J Clin Endocrinol Metab  2005  90(5):3036-44  PubMed ID: 15687336
2823  Yamauchi, Ritsuko, Morita, Akimichi, Yasuda, Yoko, Grether-Beck, Susanne, Klotz, Lars-Oliver, Tsuji, Takuo, Krutmann, Jean  Different susceptibility of malignant versus nonmalignant human T cells toward ultraviolet A-1 radiation-induced apoptosis.  J Invest Dermatol  2004  122:477-83  PubMed ID: 15009733
2894  Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6  PubMed ID: 15026424
3403  Ozaki, Kouichi, Inoue, Katsumi, Sato, Hiroshi, Iida, Aritoshi, Ohnishi, Yozo, Sekine, Akihiro, Sato, Hideyuki, Odashiro, Keita, Nobuyoshi, Masakiyo, Hori, Masatsugu, Nakamura, Yusuke, Tanaka, Toshihiro  Functional variation in LGALS2 confers risk of myocardial infarction and regulates lymphotoxin-alpha secretion in vitro.  Nature  2004  429:72-5  PubMed ID: 15129282
3340  Watanabe, Dai, Ezoe, Sachiko, Fujimoto, Minoru, Kimura, Akihiro, Saito, Yoshiyuki, Nagai, Hisaki, Tachibana, Isao, Matsumura, Itaru, Tanaka, Toshio, Kanegane, Hirokazu, Miyawaki, Toshio, Emi, Mitsuru, Kanakura, Yuzuru, Kawase, Ichiro, Naka, Tetsuji, Kishimoto, Tadamitsu  Suppressor of cytokine signalling-1 gene silencing in acute myeloid leukaemia and human haematopoietic cell lines.  Br J Haematol  2004  126:726-35  PubMed ID: 15327527
2863  Watanabe M, Kitano T, Kondo T, Yabu T, Taguchi Y, Tashima M, Umehara H, Domae N, Uchiyama T, Okazaki T.  Increase of nuclear ceramide through caspase-3-dependent regulation of the "sphingomyelin cycle" in Fas-induced apoptosis.  Cancer Res  2004  64(3):1000-7  PubMed ID: 14871831
2884  Miyoshi N, Uchida K, Osawa T, Nakamura Y.  A link between benzyl isothiocyanate-induced cell cycle arrest and apoptosis: involvement of mitogen-activated protein kinases in the Bcl-2 phosphorylation.  Cancer Res  2004  64(6):2134-42  PubMed ID: 15026354
2912  Eda S, Yamanaka M, Beppu M.  Carbohydrate-mediated phagocytic recognition of early apoptotic cells undergoing transient capping of CD43 glycoprotein.  J Biol Chem  2004  279(7):5967-74  PubMed ID: 14613931
13799  Enomoto M, Endo D, Kawashima S, Park MK.  Human type II GnRH receptor mediates effects of GnRH on cell proliferation  Zoolog Sci  2004  21(7):763-70  PubMed ID: 15277720
13800  Enomoto M, Seong JY, Kawashima S, Park MK.  Proliferation of TSU-Pr1, a human prostatic carcinoma cell line is stimulated by gonadotropin-releasing hormone  Life Sci . 2004 May 7;74(25):3141-52  2004  74(25):3141-52  PubMed ID: 15081579
2549  Nagai, Fusako, Hiyoshi, Yasuko, Sugimachi, Kumiko, Tamura, Hiro-Omi  Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines.  Biol Pharm Bull  2002  25:383-5  PubMed ID: 11913539
2819  Uchida E, Mizuguchi H, Ishii-Watabe A, Hayakawa T.  Comparison of the efficiency and safety of non-viral vector-mediated gene transfer into a wide range of human cells.  Biol Pharm Bull  2002  25(7):891-7  PubMed ID: 12132664
13865  Jiang B, Hattori N, Liu B, Kitagawa K, Inagaki C.  Expression of swelling- and/or pH-regulated chloride channels (ClC-2, 3, 4 and 5) in human leukemic and normal immune cells  Life Sci  2002  70(12):1383-94  PubMed ID: 11883714
13869  Mizuguchi H, Hayakawa T.  Adenovirus vectors containing chimeric type 5 and type 35 fiber proteins exhibit altered and expanded tropism and increase the size limit of foreign genes  Gene  2002  285(1-2):69-77.  PubMed ID: 12039033
9032  N Kiyokawa, T Mori, T Taguchi, M Saito, K Mimori, T Suzuki, T Sekino, N Sato, H Nakajima, Y U Katagiri, T Takeda, J Fujimoto  Activation of the Caspase Cascade During Stx1-induced Apoptosis in Burkitt's Lymphoma Cells  J Cell Biochem  2001  81 (1), 128-42  PubMed ID: 11180403
13864  Fujino T, Tada T, Hosaka T, Beppu M, Kikugawa K.  Presence of oxidized protein hydrolase in human cell lines, rat tissues, and human/rat plasma  J Biochem  2000  127(2):307-13  PubMed ID: 10731698
11028  Y Tomita, T Kawasaki, V Bilim, M Takeda, K Takahashi  Tetrapeptide DEVD-aldehyde or YVAD-chloromethylketone inhibits Fas/Apo-1(CD95)-mediated apoptosis in renal-cell-cancer cells  Int J Cancer  1996  68(1):132-5  PubMed ID: 8895553
6246  Ohsawa K, Watanabe Y, Sato H.  Hantaan virus persistently infects marmoset B-lymphoblastoid cell line.  J Vet Med Sci  1995  57(5):931-4  PubMed ID: 8593305



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