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Cell No. : Cell Name
RCB0804 : BRF41  update : 2024/06/20
CommentZebrafish fin fibroblast.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mitani, Hiroshi
Originator Mitani, Hiroshi
Year of deposit 1992
Another name Z41
Animal _zebrafish < Fishes
Genus Brachydanio
Species rerio
Tissue scale
Disease name fin
Classification other
Year of origin 1991
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4131
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium L-15 + 10mM HEPES + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Others Slow attachment after thawing.
deposit info
lot info
Reference information Reference 1
User's Publication 9

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18164  Abe T, Ishikawa T, Masuda T, Mizusawa K, Tsukamoto T, Mitani H, Yanagisawa T, Todo T, Iigo M.  Molecular analysis of Dec1 and Dec2 in the peripheral circadian clock of zebrafish photosensitive cells  Biochem Biophys Res Commun  2006  351(4):1072-7  PubMed ID: 17097613   DOI: 10.1016/j.bbrc.2006.10.172

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User's Publication
21594  Lanza A, Kimura S, Hirono I, Yoshitake K, Kinoshita S, Asakawa S.  Transcriptome analysis of Edwardsiella piscicida during intracellular infection reveals excludons are involved with the activation of a mitochondrion-like energy generation program.  mBio  2024  e0352623  PubMed ID: 38349189   DOI: 10.1128/mbio.03526-23
21272  Morii M, Hebiguchi T, Watanabe R, Yoshino H, Mezaki Y.  Cloning and Characterization of Cyp7a1 and Cyp27a1 Genes from the Non-Parasitic Japanese Lamprey Lethenteron reissneri.  Zoolog Sci  2023  40(3):208-218  PubMed ID: 37256568   DOI: 10.2108/zs220072
20676  Tran NT, Vo LK, Komatsu M, Shiozaki K.  Involvement of N-acetylneuraminate cytidylyltransferase in Edwardsiella piscicida pathogenicity.  Fish Shellfish Immunol  2022  124:534-542  PubMed ID: 35477099   DOI: 10.1016/j.fsi.2022.04.033
20212  Nyunoya H, Noda T, Kawamoto Y, Hayashi Y, Ishibashi Y, Ito M, Okino N.  Lack of ∆5 Desaturase Activity Impairs EPA and DHA Synthesis in Fish Cells from Red Sea Bream and Japanese Flounder.  Mar Biotechnol (NY)  2021    PubMed ID: 34176006   DOI: 10.1007/s10126-021-10040-9
18183  Fujisawa K, Terai S, Matsumoto T, Takami T, Yamamoto N, Nishina H, Furutani-Seiki M, Sakaida I.  Evidence for a Role of the Transcriptional Regulator Maid in Tumorigenesis and Aging  PLoS One  2015  10(6):e0129950  PubMed ID: 26107180   DOI: 10.1371/journal.pone.0129950
18163  Sun C, Wu Z, Jia F, Wang Y, Li T, Zhao M.  Identification of zebrafish LPTS: a gene with similarities to human LPTS/PinX1 that inhibits telomerase activity  Gene  2008  420(1):90-8  PubMed ID: 18583067   DOI: 10.1016/j.gene.2008.05.013
8845  Tamura M, Kajikawa M, Okada N.  Functional splice sites in a zebrafish LINE and their influence on zebrafish gene expression.  Gene  2007  390:221-31  PubMed ID: 17174483   DOI: 10.1016/j.gene.2006.09.003
18162  Hascilowicz T, Murai N, Matsufuji S, Murakami Y.  Regulation of ornithine decarboxylase by antizymes and antizyme inhibitor in zebrafish (Danio rerio)  Biochim Biophys Acta  2002  1578(1-3):21-8  PubMed ID: 12393184   DOI: 10.1016/s0167-4781(02)00476-1
2419  Saito T, Hascilowicz T, Ohkido I, Kikuchi Y, Okamoto H, Hayashi S, Murakami Y, Matsufuji S  Two zebrafish (Danio rerio) antizymes with different expression and activities.  Biochem J  2000  345 Pt 1:99-106  PubMed ID: 10600644   DOI: 10.1042/bj3450099

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