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Cell No. : Cell Name
RCB0804 : BRF41  update : 2022/11/25
CommentZebrafish fin fibroblast.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mitani, Hiroshi
Originator Mitani, Hiroshi
Year of deposit 1992
Another name Z41
Animal zebrafish < Fishes
Genus Brachydanio
Species rerio
Tissue scale
Disease name fin
Classification other
Year of origin 1991
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4131
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives L-15 + 10mM HEPES + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Others Slow attachment after thawing.
deposit info
lot info
Reference information Reference 0
User's Publication 4

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User's Publication
20676  Tran NT, Vo LK, Komatsu M, Shiozaki K.  Involvement of N-acetylneuraminate cytidylyltransferase in Edwardsiella piscicida pathogenicity.  Fish Shellfish Immunol  2022  124:534-542  PubMed ID: 35477099
20212  Nyunoya H, Noda T, Kawamoto Y, Hayashi Y, Ishibashi Y, Ito M, Okino N.  Lack of ∆5 Desaturase Activity Impairs EPA and DHA Synthesis in Fish Cells from Red Sea Bream and Japanese Flounder.  Mar Biotechnol (NY)  2021    PubMed ID: 34176006
8845  Tamura M, Kajikawa M, Okada N.  Functional splice sites in a zebrafish LINE and their influence on zebrafish gene expression.  Gene  2006  390:221-31  PubMed ID: 17174483
2419  Saito T, Hascilowicz T, Ohkido I, Kikuchi Y, Okamoto H, Hayashi S, Murakami Y, Matsufuji S  Two zebrafish (Danio rerio) antizymes with different expression and activities.  Biochem J  2000  345 Pt 1:99-106  PubMed ID: 10600644

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