Cell No. : Cell Name
							 RCB0804 : BRF41
						
						 update : 2025/05/09
						
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								| Comment | Zebrafish fin fibroblast. | 
							
							
								| Comment from the depositor |  | 
							
							
								| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | 
							
							
								| Remarks |  | 
							
							
							
								| Order Form | 
								
									
										
											
										
										Order Form(C-0005.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007p.pdf)
										
											
										
										 
									
								 | 
							
							
								| 
									Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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								Basic information
							
							
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                                        Depositor | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Mitani, Hiroshi
							
							
						
						
							
						
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                                        | Originator | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Mitani, Hiroshi
							
							
						
						
							
						
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                                        | Year of deposit | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								1992
							
							
						
						
							
						
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                                        | Another name | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Z41
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Animal | 
                                
                                
				
				
				
					
						
						
							
								 
								
								 	_zebrafish
								
								
								
							
								 
								
								
								 	
								 		< Fishes
								 	
								
								
							
						
						
						
						
						
							
						
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                                        | Genus | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Brachydanio
							
							
						
						
							
						
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                                        | Species | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								rerio
							
							
						
						
							
						
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                                        | Tissue | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								scale
							
							
						
						
							
						
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                                        | Disease name | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								fin
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Classification | 
                                
                                
				
				
				
					
						
						
						
							other
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Year of origin | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								1991
							
							
						
						
							
						
					 | 
				
				
					
					
									
								
									
								
									
								
									
								
							
						
					
						
						
						
							
							
							
							
								
							
							
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                                        Lifespan | 
                                
                                
				
				
				
					
						
						
						
							infinite
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Morphology | 
                                
                                
				
				
				
					
						
						
						
							fibroblast-like
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Cellosaurus(Expasy) | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								CVCL_4131
							
							
						
						
							
						
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											deposit info | 
											lot info | 
											
												 | 
												Medium | 
												Medium List | 
											
										
									
								
						
						
						
						
						
					
						
						
						
						
							
							
							
							
								
							
							
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					Culture type | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							Adherent cells
						
						
						
						
							
						
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					| Culture medium | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								L-15 + 10mM HEPES + 15% FBS
							
							
						
						
							
						
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					| Antibiotics | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Free
							
							
						
						
							
						
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					Passage method | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
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								0.25% Trypsin
							
							
						
						
							
						
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								Culture information
							
							
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					Passage ratio | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
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								1 : 5 split
							
							
						
						
							
						
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					| SC frequency | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Subculture : once/10 days, Medium Renewal : 2 times/week
							
							
						
						
							
						
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					| Temperature | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
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								33
							
							
						
						
							℃
						
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					| CO2 concentration | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								0
							
							
						
						
							%
						
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					| Freeze medium | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Medium + 10% DMSO
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freezing method | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							Slow freezing
						
						
						
						
							
						
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					| Mycoplasma/Acholeplasma | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							(-)
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| DNA barcoding | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								OK
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Others | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Slow attachment after thawing.
							
							
						
						
							
						
					 | 
				
				
					
					
									
								
							
						
					
					
					
					
					
			
			
			
			
					
						
							
							
							
								| Images | 
								deposit info | lot info | 
							
							
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								| Reference information | 
								Reference | 
									
									
									
										2
									
									
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								| User's Publication | 
									
									
									
										11
									
									
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						| Reference | 
						
							
							| 
								
								
								
								23367 
								Kumar MS, Singh VK, Mishra AK, Kushwaha B, Kumar R, Lal KK. 
								Fish cell line: depositories, web resources and future applications 
								Cytotechnology 
								2024 
								76(1):1-25 
								
									
										PubMed ID: 38304629
									
								 
								
									
										DOI: 10.1007/s10616-023-00601-2
									
								
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								18164 
								Abe T, Ishikawa T, Masuda T, Mizusawa K, Tsukamoto T, Mitani H, Yanagisawa T, Todo T, Iigo M. 
								Molecular analysis of Dec1 and Dec2 in the peripheral circadian clock of zebrafish photosensitive cells 
								Biochem Biophys Res Commun 
								2006 
								351(4):1072-7 
								
									
										PubMed ID: 17097613
									
								 
								
									
										DOI: 10.1016/j.bbrc.2006.10.172
									
								
							 | 
							
						
					
				
					
						| User's Publication | 
						
							
							| 
								
								
								22672 
								Xu Z, Asakawa S. 
								Release and degradation of dissolved environmental RNAs from zebrafish cells. 
								RNA Biol 
								2025 
								 
								
									
										PubMed ID: 40167163
									
								 
								
									
										DOI: 10.1080/15476286.2025.2486281
									
								
							 | 
							
						
							
							| 
								
								
								22974 
								Fukushima HS, Takeda H. 
								Coordinated action of multiple active histone modifications shapes the zygotic genome activation in teleost embryos. 
								Nat Commun 
								2025 
								16(1):5222 
								
									
										PubMed ID: 40523893
									
								 
								
									
										DOI: 10.1038/s41467-025-60246-x
									
								
							 | 
							
						
							
							| 
								
								
								21594 
								Lanza A, Kimura S, Hirono I, Yoshitake K, Kinoshita S, Asakawa S. 
								Transcriptome analysis of Edwardsiella piscicida during intracellular infection reveals excludons are involved with the activation of a mitochondrion-like energy generation program. 
								mBio 
								2024 
								e0352623 
								
									
										PubMed ID: 38349189
									
								 
								
									
										DOI: 10.1128/mbio.03526-23
									
								
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								21272 
								Morii M, Hebiguchi T, Watanabe R, Yoshino H, Mezaki Y. 
								Cloning and Characterization of Cyp7a1 and Cyp27a1 Genes from the Non-Parasitic Japanese Lamprey Lethenteron reissneri. 
								Zoolog Sci 
								2023 
								40(3):208-218 
								
									
										PubMed ID: 37256568
									
								 
								
									
										DOI: 10.2108/zs220072
									
								
							 | 
							
						
							
							| 
								
								
								20676 
								Tran NT, Vo LK, Komatsu M, Shiozaki K. 
								Involvement of N-acetylneuraminate cytidylyltransferase in Edwardsiella piscicida pathogenicity. 
								Fish Shellfish Immunol 
								2022 
								124:534-542 
								
									
										PubMed ID: 35477099
									
								 
								
									
										DOI: 10.1016/j.fsi.2022.04.033
									
								
							 | 
							
						
							
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								20212 
								Nyunoya H, Noda T, Kawamoto Y, Hayashi Y, Ishibashi Y, Ito M, Okino N. 
								Lack of ∆5 Desaturase Activity Impairs EPA and DHA Synthesis in Fish Cells from Red Sea Bream and Japanese Flounder. 
								Mar Biotechnol (NY) 
								2021 
								 
								
									
										PubMed ID: 34176006
									
								 
								
									
										DOI: 10.1007/s10126-021-10040-9
									
								
							 | 
							
						
							
							| 
								
								
								18183 
								Fujisawa K, Terai S, Matsumoto T, Takami T, Yamamoto N, Nishina H, Furutani-Seiki M, Sakaida I. 
								Evidence for a Role of the Transcriptional Regulator Maid in Tumorigenesis and Aging 
								PLoS One 
								2015 
								10(6):e0129950 
								
									
										PubMed ID: 26107180
									
								 
								
									
										DOI: 10.1371/journal.pone.0129950
									
								
							 | 
							
						
							
							| 
								
								
								18163 
								Sun C, Wu Z, Jia F, Wang Y, Li T, Zhao M. 
								Identification of zebrafish LPTS: a gene with similarities to human LPTS/PinX1 that inhibits telomerase activity 
								Gene 
								2008 
								420(1):90-8 
								
									
										PubMed ID: 18583067
									
								 
								
									
										DOI: 10.1016/j.gene.2008.05.013
									
								
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								8845 
								Tamura M, Kajikawa M, Okada N. 
								Functional splice sites in a zebrafish LINE and their influence on zebrafish gene expression. 
								Gene 
								2007 
								390:221-31 
								
									
										PubMed ID: 17174483
									
								 
								
									
										DOI: 10.1016/j.gene.2006.09.003
									
								
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								18162 
								Hascilowicz T, Murai N, Matsufuji S, Murakami Y. 
								Regulation of ornithine decarboxylase by antizymes and antizyme inhibitor in zebrafish (Danio rerio) 
								Biochim Biophys Acta 
								2002 
								1578(1-3):21-8 
								
									
										PubMed ID: 12393184
									
								 
								
									
										DOI: 10.1016/s0167-4781(02)00476-1
									
								
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								2419 
								Saito T, Hascilowicz T, Ohkido I, Kikuchi Y, Okamoto H, Hayashi S, Murakami Y, Matsufuji S 
								Two zebrafish (Danio rerio) antizymes with different expression and activities. 
								Biochem J 
								2000 
								345 Pt 1:99-106 
								
									
										PubMed ID: 10600644
									
								 
								
									
										DOI: 10.1042/bj3450099
									
								
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