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Cell No. : Cell Name
RCB0796 : 1C3D3  update : 2022/03/25
CommentHuman cell line derived from embryonic pancreas. Four cell lines (RCB0794,RCB0795,RCB0796,RCB0797) were derived from the same individual.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.ISHIKAWA Hiroshi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, Hiroshi
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Unknown
Age at sampling 19 weeks embryo
Tissue embryo, pancreas
Classification other
Year of origin 1991
History Direct from originator
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan finite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U823
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF10 + 5% FBS + 10% NBS + 2.5% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 46-171(50) : /51(2),52(6),53(5),54(3),55(4),56(2)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 4


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Reference

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User's Publication
16522  Kawatani M, Aono H, Hiranuma S, Shimizu T, Muroi M, Ogawa N, Ohishi T, Ohba SI, Kawada M, Nogawa T, Okano A, Hashizume D, Osada H.  Identification of a Small-Molecule Glucose Transporter Inhibitor, Glutipyran, That Inhibits Cancer Cell Growth  ACS Chem Biol  2021  16(8):1576-1586  PubMed ID: 34296611   DOI: 10.1021/acschembio.1c00480
9713  Sakamoto R, Kakinuma E, Masuda K, Takeuchi Y, Ito K, Iketaki K, Matsuzaki T, Nakabayashi S, Yoshikawa HY, Yamamoto H, Sato Y, Tanii T.  Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: an experimental study on the anti-adhesion effect of green-tea catechins.  In Vitro Cell. Dev. Biol. Anim.  2016  52:799-805  PubMed ID: 27251159   DOI: 10.1007/s11626-016-0049-6
7928  Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550   DOI: 10.1002/jso.21812
5875  Mishra R, Miyamoto M, Yoshioka T, Ishikawa K, Matsumura Y, Shoji Y, Ichinokawa K, Itoh T, Shichinohe T, Hirano S, Kondo S.  Adenovirus-mediated eukaryotic initiation factor 4E binding protein-1 in combination with rapamycin inhibits tumor growth of pancreatic ductal adenocarcinoma in vivo.  Int J Oncol  2009  34(5):1231-40  PubMed ID: 19360336  


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