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Cell No. : Cell Name
RCB0794 : 2C6  update : 2022/09/15
CommentHuman cell line derived from embryonic pancreas. Four cell lines (RCB0794,RCB0795,RCB0796,RCB0797) were derived from the same individual.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.ISHIKAWA Hiroshi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, Hiroshi
Year of deposit 1992
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Unknown
Age at sampling 19 weeks embryo
Tissue embryo, pancreas
Classification other
Year of origin 1991
History Direct from originator.
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan finite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U825
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF10 + 5% FBS + 10% NBS + 2.5% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 57-122(50) : /59(4),108(3),111(5),112(4),113(4)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
21379  Hata M, Ueno J, Hitomi Y, Kodera M.  Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates.  ACS Omega  2023  8(31):28690-28701  PubMed ID: 37576680   DOI: 10.1021/acsomega.3c03387



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