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Cell No. : Cell Name
RCB0793 : B151K12  update : 2022/03/24
CommentIL-5 producing T-cell hybridoma. Culture sup is useful for T88-M cell culture.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
approver's address
The Institute of Medical Science, The University of Tokyo
Department of Microbiology and Immunology Division of Immunology
4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
Dr.TAKATSU Kiyoshi
Fax. +81-3-3446-6068

Osaka University Graduate School
1-3, Yamadaoka, Suita-Shi, Osaka-Fu, 565-0871, JAPAN
Dr.TANAKA Kiyoji
Fax. +81-6-877-9136
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Tominaga, Akira
Originator Tanaka, K. & Takatu, K.
Year of deposit 1992
Animal _mouse x mouse < Hybrid
Tissue T cell hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U121
hybridoma Fusion_cell_1 name Helper T
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name BW5147-AZD-r,OU-r
Fusion_cell_2 strain mouse, AKR
Fusion method Sendai virus, HAT selection
Immunogen Myco.tuberculosis
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 15
User's Publication 0

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133  Takatsu K, Yamaguchi N, Hitoshi Y, Sonoda E, Mita S, Tominaga A.  Transgenic mice expressing a B cell growth and differentiation factor gene (interleukin 5) develop eosinophilia and autoantibody production  J Exp Med  1991  173(2):429-37  PubMed ID: 1988543   DOI: 10.1084/jem.173.2.429
4826  Takahashi T, Yamaguchi N, Mita S, Yamaguchi Y, Suda T, Tominaga A, Kikuchi Y, Miura Y, Takatsu K.  Structural comparison of murine T-cell (B151K12)-derived T-cell-replacing factor (IL-5) with rIL-5: dimer formation is essential for the expression of biological activity.  Mol Immunol  1990  27(9):911-20  PubMed ID: 2215480   DOI: 10.1016/0161-5890(90)90158-v
5198  Tominaga A, Takahashi T, Kikuchi Y, Mita S, Naomi S, Harada N, Yamaguchi N, Takatsu K.  Role of carbohydrate moiety of IL-5. Effect of tunicamycin on the glycosylation of IL-5 and the biologic activity of deglycosylated IL-5.  J Immunol  1990  144(4):1345-52  PubMed ID: 2303708  
4819  Mita S, Harada N, Naomi S, Hitoshi Y, Sakamoto K, Akagi M, Tominaga A, Takatsu K.  Receptors for T cell-replacing factor/interleukin 5. Specificity, quantitation, and its implication.  J Exp Med  1988  168(3):863-78  PubMed ID: 3262707   DOI: 10.1084/jem.168.3.863
4824  Tominaga A, Matsumoto M, Harada N, Takahashi T, Kikuchi Y, Takatsu K.  Molecular properties and regulation of mRNA expression for murine T cell-replacing factor/IL-5.  J Immunol  1988  140(4):1175-81  PubMed ID: 2893824  
4825  Murakami S, Ono S, Harada N, Hara Y, Katoh Y, Dobashi K, Takatsu K, Hamaoka T.  T-cell-derived factor B151-TRF1/IL-5 activates blastoid cells among unprimed B cells to induce a polyclonal differentiation into immunoglobulin M-secreting cells.  Immunology  1988  65(2):221-8  PubMed ID: 3263941  
141  Koyama N, Harada N, Takahashi T, Mita S, Okamura H, Tominaga A, Takatsu K.  Role of recombinant interleukin-1 compared to recombinant T-cell replacing factor/interleukin-5 in B-cell differentiation  Immunology  1988  63(2):277-83  PubMed ID: 3280472  
4823  Matsumoto M, Tominaga A, Harada N, Takatsu K.  Role of T cell-replacing factor (TRF) in the murine B cell differentiation: induction of increased levels of expression of secreted type IgM mRNA.  J Immunol  1987  138(6):1826-33  PubMed ID: 3102602  
278  Takatsu K, Kikuchi Y, Takahashi T, Honjo T, Matsumoto M, Harada N, Yamaguchi N, Tominaga A.  Interleukin 5, a T-cell-derived B-cell differentiation factor also induces cytotoxic T lymphocytes  Proc Natl Acad Sci U S A  1987  84(12):4234-8  PubMed ID: 3495803   DOI: 10.1073/pnas.84.12.4234
142  Alderson MR, Pike BL, Harada N, Tominaga A, Takatsu K, Nossal GJ.  Recombinant T cell replacing factor (interleukin 5) acts with antigen to promote the growth and differentiation of single hapten-specific B lymphocytes  J Immunol  1987  139(8):2656-60  PubMed ID: 3498766  
4822  Tominaga A, Matsumoto M, Takahashi T, Harada N, Takatsu K.  Establishment of an assay system for the detection of translated materials of T-cell-replacing factor mRNA in Xenopus oocytes.  Microbiol Immunol  1986  30(8):789-98  PubMed ID: 2878350   DOI: 10.1111/j.1348-0421.1986.tb03005.x
138  Kinashi T, Harada N, Severinson E, Tanabe T, Sideras P, Konishi M, Azuma C, Tominaga A, Bergstedt-Lindqvist S, Takahashi M.  Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II  Nature  1986  324(6092):70-3  PubMed ID: 3024009   DOI: 10.1038/324070a0
4821  Harada N, Kikuchi Y, Tominaga A, Takaki S, Takatsu K.  BCGFII activity on activated B cells of a purified murine T cell-replacing factor (TRF) from a T cell hybridoma (B151K12).  J Immunol  1985  134(6):3944-51  PubMed ID: 3872907  
139  Kinashi T, Harada N, Severinson E, Tanabe T, Sideras P, Konishi M, Azuma C, Tominaga A, Bergstedt-Lindqvist S, Takahashi M.  Acceptor site(s) for T cell-replacing factor (TRF) on B lymphocytes. II. Activation of B cells by cross-linkage or aggregation of the TRF acceptor molecule  J Immunol  1982  128(6):2581-5  PubMed ID: 6978908  
1984  Takatsu K, Tanaka K, Tominaga A, Kumahara Y, Hamaoka T  Antigen-induced T cell-replacing factor (TRF). III. Establishment of T cell hybrid clone continuously producing TRF and functional analysis of released TRF.  J Immunol  1980  125:2646-53  PubMed ID: 6159416  

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