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Cell No. : Cell Name
RCB0792 : T88-M  update : 2024/08/14
CommentIL-5 dependent early B-cell line. Also grows on IL-3. The population contain non-IL-5-dependent cell
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tominaga, Akira
Originator Tominaga, Akira
Year of deposit 1992
Animal _mouse < Mammals
Gender Female
Age at sampling 3 weeks
Tissue B cell
Disease name B cell leukemia
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_6542
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS + 40pg/ml rmouse IL-5 (or 10% RCB0793 B151K12 conditioned medium)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
SSLP(mouse) OK
Virus (HIV) (-)
Isozyme LD, NP
Reference information Reference 19
User's Publication 2


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Reference
5205  Ohbo K, Asao H, Kouro T, Nakamura M, Takaki S, Kikuchi Y, Hirokawa K, Tominaga A, Takatsu K, Sugamura K.  Demonstration of a cross-talk between IL-2 and IL-5 in phosphorylation of IL-2 and IL-5 receptor beta chains.  Int Immunol  1996  8(6):951-60  PubMed ID: 8671684   DOI: 10.1093/intimm/8.6.951
136  Imamura F, Takaki S, Akagi K, Ando M, Yamamura K, Takatsu K, Tominaga A.  The murine interleukin-5 receptor alpha-subunit gene: characterization of the gene structure and chromosome mapping  DNA Cell Biol  1994  13(3):283-92  PubMed ID: 8172657   DOI: 10.1089/dna.1994.13.283
5204  Mita S1, Takaki S, Tominaga A, Takatsu K.  Comparative analysis of the kinetics of binding and internalization of IL-5 in murine IL-5 receptors of high and low affinity.  J Immunol  1993  151(12):6924-32  PubMed ID: 8258700  
134  Tominaga A, Takaki S, Hitoshi Y, Takatsu K.  Role of the interleukin 5 receptor system in hematopoiesis: molecular basis for overlapping function of cytokines  Bioessays  1992  14(8):527-33  PubMed ID: 1365906   DOI: 10.1002/bies.950140806
1988  Yamaguchi N, Hitoshi Y, Takaki S, Murata Y, Migita M, Kamiya T, Minowada J, Tominaga A, Takatsu K  Murine interleukin 5 receptor isolated by immunoaffinity chromatography: comparison of determined N-terminal sequence and deduced primary sequence from cDNA and implication of a role of the intracytoplasmic domain.  Int Immunol  1991  3:889-98  PubMed ID: 1931815   DOI: 10.1093/intimm/3.9.889
277  Takatsu K, Tominaga A.  Interleukin 5 and its receptor  Prog Growth Factor Res  1991  3(2):87-102  PubMed ID: 1773042   DOI: 10.1016/s0955-2235(05)80001-8
130  Mita S, Takaki S, Hitoshi Y, Rolink AG, Tominaga A, Yamaguchi N, Takatsu K.  Molecular characterization of the beta chain of the murine interleukin 5 receptor  Int Immunol  1991  3(7):665-72  PubMed ID: 1911539   DOI: 10.1093/intimm/3.7.665
131  Migita M, Yamaguchi N, Mita S, Higuchi S, Hitoshi Y, Yoshida Y, Tomonaga M, Matsuda I, Tominaga A, Takatsu K.  Characterization of the human IL-5 receptors on eosinophils  Cell Immunol  1991  133(2):484-97  PubMed ID: 2015632   DOI: 10.1016/0008-8749(91)90120-z
133  Takatsu K, Yamaguchi N, Hitoshi Y, Sonoda E, Mita S, Tominaga A.  Transgenic mice expressing a B cell growth and differentiation factor gene (interleukin 5) develop eosinophilia and autoantibody production  J Exp Med  1991  173(2):429-37  PubMed ID: 1988543   DOI: 10.1084/jem.173.2.429
137  Takaki S, Mita S, Kitamura T, Yonehara S, Yamaguchi N, Tominaga A, Miyajima A, Takatsu K.  Identification of the second subunit of the murine interleukin-5 receptor: interleukin-3 receptor-like protein, AIC2B is a component of the high affinity interleukin-5 receptor  EMBO J  1991  10(10):2833-8  PubMed ID: 1915265   DOI: 10.1002/j.1460-2075.1991.tb07832.x
4826  Takahashi T, Yamaguchi N, Mita S, Yamaguchi Y, Suda T, Tominaga A, Kikuchi Y, Miura Y, Takatsu K.  Structural comparison of murine T-cell (B151K12)-derived T-cell-replacing factor (IL-5) with rIL-5: dimer formation is essential for the expression of biological activity.  Mol Immunol  1990  27(9):911-20  PubMed ID: 2215480   DOI: 10.1016/0161-5890(90)90158-v
5198  Tominaga A, Takahashi T, Kikuchi Y, Mita S, Naomi S, Harada N, Yamaguchi N, Takatsu K.  Role of carbohydrate moiety of IL-5. Effect of tunicamycin on the glycosylation of IL-5 and the biologic activity of deglycosylated IL-5.  J Immunol  1990  144(4):1345-52  PubMed ID: 2303708  
5200  Hitoshi Y, Yamaguchi N, Mita S, Sonoda E, Takaki S, Tominaga A, Takatsu K.  Distribution of IL-5 receptor-positive B cells. Expression of IL-5 receptor on Ly-1(CD5)+ B cells.  J Immunol  1990  144(11):4218-25  PubMed ID: 1692859  
5201  Murata Y1, Yamaguchi N, Hitoshi Y, Tominaga A, Takatsu K.  Interleukin 5 and interleukin 3 induce serine and tyrosine phosphorylations of several cellular proteins in an interleukin 5-dependent cell line.  Biochem Biophys Res Commun  1990  173(3):1102-8  PubMed ID: 2125214   DOI: 10.1016/s0006-291x(05)80899-x
5202  Yamaguchi N, Hitoshi Y, Mita S, Hosoya Y, Murata Y, Kikuchi Y, Tominaga A, Takatsu K.  Characterization of the murine interleukin 5 receptor by using a monoclonal antibody.  Int Immunol  1990  2(2):181-7  PubMed ID: 2088484   DOI: 10.1093/intimm/2.2.181
1987  Tominaga A, Mita S, Kikuchi Y, Hitoshi Y, Takatsu K, Nishikawa S, Ogawa M  Establishment of IL-5-dependent early B cell lines by long-term bone marrow cultures.  Growth Factors  1989  1:135-46  PubMed ID: 2624778   DOI: 10.3109/08977198909029123
1985  Mita S, Tominaga A, Hitoshi Y, Sakamoto K, Honjo T, Akagi M, Kikuchi Y, Yamaguchi N, Takatsu K  Characterization of high-affinity receptors for interleukin 5 on interleukin 5-dependent cell lines.  Proc Natl Acad Sci U S A  1989  86:2311-5  PubMed ID: 2784567   DOI: 10.1073/pnas.86.7.2311
132  Takatsu K, Yamaguchi N, Hitoshi Y, Sonoda E, Mita S, Tominaga A.  Signal transduction through interleukin-5 receptors  Cold Spring Harb Symp Quant Biol  1989  54 Pt 2:745-51  PubMed ID: 2640627   DOI: 10.1101/sqb.1989.054.01.088
4819  Mita S, Harada N, Naomi S, Hitoshi Y, Sakamoto K, Akagi M, Tominaga A, Takatsu K.  Receptors for T cell-replacing factor/interleukin 5. Specificity, quantitation, and its implication.  J Exp Med  1988  168(3):863-78  PubMed ID: 3262707   DOI: 10.1084/jem.168.3.863

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User's Publication
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014    DOI: 10.4051/ibc.2010.2.4.0014
3472  Ishihara K, Satoh I, Nittoh T, Kanaya T, Okazaki H, Suzuki T, Koyama T, Sakamoto T, Ide T, Ohuchi K  Preparation of recombinant rat interleukin-5 by baculovirus expression system and analysis of its biological activities.  Biochim Biophys Acta  1999  1451:48-58  PubMed ID: 10446387   DOI: 10.1016/s0167-4889(99)00090-7


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