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Cell No. : Cell Name
RCB0792 : T88-M  update : 2022/03/24
CommentIL-5 dependent early B-cell line. Also grows on IL-3. The population contain non-IL-5-dependent cell
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Tominaga, Akira
Originator Tominaga, Akira
Year of deposit 1992
Animal mouse < Mammals
Gender Female
Age at sampling 3 weeks
Tissue B cell
Disease name B cell leukemia
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_6542
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 40pg/ml rmouse IL-5 (or 10% RCB0793 B151K12 conditioned medium)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Virus (HIV) (-)
Isozyme LD, NP
Reference information Reference 10
User's Publication 2

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5205  Ohbo K, Asao H, Kouro T, Nakamura M, Takaki S, Kikuchi Y, Hirokawa K, Tominaga A, Takatsu K, Sugamura K.  Demonstration of a cross-talk between IL-2 and IL-5 in phosphorylation of IL-2 and IL-5 receptor beta chains.  Int Immunol  1996  8(6):951-60  PubMed ID: 8671684   DOI: 10.1093/intimm/8.6.951
5204  Mita S1, Takaki S, Tominaga A, Takatsu K.  Comparative analysis of the kinetics of binding and internalization of IL-5 in murine IL-5 receptors of high and low affinity.  J Immunol  1993  151(12):6924-32  PubMed ID: 8258700  
1988  Yamaguchi N, Hitoshi Y, Takaki S, Murata Y, Migita M, Kamiya T, Minowada J, Tominaga A, Takatsu K  Murine interleukin 5 receptor isolated by immunoaffinity chromatography: comparison of determined N-terminal sequence and deduced primary sequence from cDNA and implication of a role of the intracytoplasmic domain.  Int Immunol  1991  3:889-98  PubMed ID: 1931815   DOI: 10.1093/intimm/3.9.889
5198  Tominaga A, Takahashi T, Kikuchi Y, Mita S, Naomi S, Harada N, Yamaguchi N, Takatsu K.  Role of carbohydrate moiety of IL-5. Effect of tunicamycin on the glycosylation of IL-5 and the biologic activity of deglycosylated IL-5.  J Immunol  1990  144(4):1345-52  PubMed ID: 2303708  
5200  Hitoshi Y, Yamaguchi N, Mita S, Sonoda E, Takaki S, Tominaga A, Takatsu K.  Distribution of IL-5 receptor-positive B cells. Expression of IL-5 receptor on Ly-1(CD5)+ B cells.  J Immunol  1990  144(11):4218-25  PubMed ID: 1692859  
5201  Murata Y1, Yamaguchi N, Hitoshi Y, Tominaga A, Takatsu K.  Interleukin 5 and interleukin 3 induce serine and tyrosine phosphorylations of several cellular proteins in an interleukin 5-dependent cell line.  Biochem Biophys Res Commun  1990  173(3):1102-8  PubMed ID: 2125214   DOI: 10.1016/s0006-291x(05)80899-x
5202  Yamaguchi N, Hitoshi Y, Mita S, Hosoya Y, Murata Y, Kikuchi Y, Tominaga A, Takatsu K.  Characterization of the murine interleukin 5 receptor by using a monoclonal antibody.  Int Immunol  1990  2(2):181-7  PubMed ID: 2088484   DOI: 10.1093/intimm/2.2.181
1987  Tominaga A, Mita S, Kikuchi Y, Hitoshi Y, Takatsu K, Nishikawa S, Ogawa M  Establishment of IL-5-dependent early B cell lines by long-term bone marrow cultures.  Growth Factors  1989  1:135-46  PubMed ID: 2624778  
1985  Mita S, Tominaga A, Hitoshi Y, Sakamoto K, Honjo T, Akagi M, Kikuchi Y, Yamaguchi N, Takatsu K  Characterization of high-affinity receptors for interleukin 5 on interleukin 5-dependent cell lines.  Proc Natl Acad Sci U S A  1989  86:2311-5  PubMed ID: 2784567   DOI: 10.1073/pnas.86.7.2311
4819  Mita S, Harada N, Naomi S, Hitoshi Y, Sakamoto K, Akagi M, Tominaga A, Takatsu K.  Receptors for T cell-replacing factor/interleukin 5. Specificity, quantitation, and its implication.  J Exp Med  1988  168(3):863-78  PubMed ID: 3262707   DOI: 10.1084/jem.168.3.863

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User's Publication
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014    DOI: 10.4051/ibc.2010.2.4.0014
3472  Ishihara K, Satoh I, Nittoh T, Kanaya T, Okazaki H, Suzuki T, Koyama T, Sakamoto T, Ide T, Ohuchi K  Preparation of recombinant rat interleukin-5 by baculovirus expression system and analysis of its biological activities.  Biochim Biophys Acta  1999  1451:48-58  PubMed ID: 10446387   DOI: 10.1016/s0167-4889(99)00090-7

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