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細胞番号 : 細胞名
RCB0778 : CW-2  update : 2024/06/01
細胞特性(Comment:英)Colon carcinoma. CEA-positive, nude mouse-transplantable.
細胞特性(日)日本人由来大腸癌細胞株。CEA産生、ヌードマウス移植可能という。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Mitsuhashi, Maki
樹立者 Mitsuhashi, Maki
寄託日 1992
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Female
55 years
採取組織 colon
細胞分類 cancer
初代培養日 1990
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_1151
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 RPMI1640 + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
染色体検査 44-92(50) : 44(1),45(5),46(42),47(1),92(1)
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 1件
Reference(日) 1件
利用者成果(英) 48件
利用者成果(日) 1件

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Reference(英)
129  Maki Mitsuhashi, Katsumi Yamauchi, Takaji Furukawa, Kyouichi Hamano  Enhancement of tumor-specific cytotoxic T lymphocyte activity in cancer patients by pretreatment with cyclophosphamide  Annals of Cancer Research and Therapy  1994  3:97-101,69    DOI: 10.4993/acrt1992.3.97

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Reference(日)
1674  三橋牧、他  ヒト胃癌培養株2例の樹立とその性状    1991     

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利用者成果(英)
19943  Koyama Y, Fujihara S, Chiyo T, Matsui T, Hamaya S, Fujita K, Tani J, Morishita A, Kobara H, Ono M, Iwama H, Masaki T.  Role of Mir-452-5p Overexpression in Epithelial-Mesenchymal Transition (EMT) in Early-stage Colorectal Cancer  In Vivo  2023  37(5):1980-1990  PubMed ID: 37652512   DOI: 10.21873/invivo.13295
19975  Aiba T, Hijiya N, Akagi T, Tsukamoto Y, Hirashita Y, Kinoshita K, Uchida T, Nakada C, Kurogi S, Ueda Y, Shiroshita H, Shiraishi N, Murakami K, Inomata M, Moriyama M.  Overexpression of VSNL1 enhances cell proliferation in colorectal carcinogenesis  Pathobiology  2023  Oct 5  PubMed ID: 37797604   DOI: 10.1159/000533877
20203  Kast F, Schwill M, Stüber JC, Pfundstein S, Nagy-Davidescu G, Rodríguez JMM, Seehusen F, Richter CP, Honegger A, Hartmann KP, Weber TG, Kroener F, Ernst P, Piehler J, Plückthun A.  Engineering an anti-HER2 biparatopic antibody with a multimodal mechanism of action.  Nat Commun  2021  12(1):3790  PubMed ID: 34145240   DOI: 10.1038/s41467-021-23948-6
15080  Hirashita Y, Tsukamoto Y, Kudo Y, Kakisako D, Kurogi S, Hijiya N, Nakada C, Uchida T, Hirashita T, Hiratsuka T, Akagi T, Ueda Y, Shiroshita H, Etoh T, Mizukami K, Honda K, Okimoto T, Kodama M, Inomata M, Moriyama M, Murakami K.  Early response in phosphorylation of ribosomal protein S6 is associated with sensitivity to trametinib in colorectal cancer cells  Lab Invest  2021  101(8):1036-1047  PubMed ID: 33911189   DOI: 10.1038/s41374-021-00590-w
15088  Hanker AB, Brown BP, Meiler J, Marín A, Jayanthan HS, Ye D, Lin CC, Akamatsu H, Lee KM, Chatterjee S, Sudhan DR, Servetto A, Brewer MR, Koch JP, Sheehan JH, He J, Lalani AS, Arteaga CL.  Co-occurring gain-of-function mutations in HER2 and HER3 modulate HER2/HER3 activation, oncogenesis, and HER2 inhibitor sensitivity  Cancer Cell  2021  39(8):1099-1114.e8  PubMed ID: 34171264   DOI: 10.1016/j.ccell.2021.06.001
9913  Satoshi Inoue, Takumi Tsunoda, Miho Riku, Hideaki Ito, Akihito Inoko, Hideki Murakami, Masahide Ebi, Naotaka Ogasawara, Ira Pastan, Kunio Kasugai, Kenji Kasai, Hiroshi Ikeda, Shingo Inaguma  Diffuse Mesothelin Expression Leads to Worse Prognosis Through Enhanced Cellular Proliferation in Colorectal Cancer  Oncol Lett  2020  19(3):1741-1750  PubMed ID: 32194667   DOI: 10.3892/ol.2020.11290
11927  Yang J, Isaji T, Zhang G, Qi F, Duan C, Fukuda T, Gu J.  EpCAM associates with integrin and regulates cell adhesion in cancer cells.  Biochem. Biophys. Res. Commun.  2020    PubMed ID: 31806375   DOI: 10.1016/j.bbrc.2019.11.152
13234  Nishimoto A, Takemoto Y, Saito T, Kurazumi H, Tanaka T, Harada E, Shirasawa B, Hamano K.  Nuclear β-catenin expression is positively regulated by JAB1 in human colorectal cancer cells.  Biochem Biophys Res Commun  2020    PubMed ID: 32977947   DOI: 10.1016/j.bbrc.2020.09.007
13325  Fernando TM, Piskol R, Bainer R, Sokol ES, Trabucco SE, Zhang Q, Trinh H, Maund S, Kschonsak M, Chaudhuri S, Modrusan Z, Januario T, Yauch RL.  Functional characterization of SMARCA4 variants identified by targeted exome-sequencing of 131,668 cancer patients.  Nat Commun  2020    PubMed ID: 33144586   DOI: 10.1038/s41467-020-19402-8
11342  Washio I, Nakanishi T, Ishiguro N, Bister B, Tamai I.  Effect of endogenous multidrug resistance 1 and P-glycoprotein expression on anticancer drug resistance in colon cancer cell lines.  Biopharm Drug Dispos  2019    PubMed ID: 30556139   DOI: 10.1002/bdd.2167
11364  Kimura T, Tsukada A, Fukutomi T, Ichida K, Ohtsuki S, Sakurai H.  Urate Transport via Paracellular Route across Epithelial Cells.  Biol. Pharm. Bull.  2019    PubMed ID: 30606989   DOI: 10.1248/bpb.b18-00505
11553  Wu Z, Ichinose T, Naoe Y, Matsumura S, Villalobos IB, Eissa IR, Yamada S, Miyajima N, Morimoto D, Mukoyama N, Nishikawa Y, Koide Y, Kodera Y, Tanaka M, Kasuya H.  Combination of Cetuximab and Oncolytic Virus Canerpaturev Synergistically Inhibits Human Colorectal Cancer Growth.  Mol Ther Oncolytics  2019    PubMed ID: 31193737   DOI: 10.1016/j.omto.2019.04.004
11832  Robichaux JP, Elamin YY, Vijayan RSK, Nilsson MB, Hu L, He J, Zhang F, Pisegna M, Poteete A, Sun H, Li S, Chen T, Han H, Negrao MV, Ahnert JR, Diao L, Wang J, Le X, Meric-Bernstam F, Routbort M, Roeck B, Yang Z, Raymond VM, Lanman RB, Frampton GM, Miller VA, Schrock AB, Albacker LA, Wong KK, Cross JB, Heymach JV.  Pan-Cancer Landscape and Analysis of ERBB2 Mutations Identifies Poziotinib as a Clinically Active Inhibitor and Enhancer of T-DM1 Activity.  Cancer Cell  2019    PubMed ID: 31588020   DOI: 10.1016/j.ccell.2019.09.001
4178  Ichimanda M, Hijiya N, Tsukamoto Y, Uchida T, Nakada C, Akagi T, Etoh T, Iha H, Inomata M, Takekawa M, Moriyama M.  Downregulation of dual-specificity phosphatase 4 enhances cell proliferation and invasiveness in colorectal carcinomas.  Cancer Sci.  2018    PubMed ID: 29150975   DOI: 10.1111/cas.13444
10398  Matsushita N, Yamamoto S, Inoue Y, Aruga A, Yamamoto M.  RT-qPCR analysis of the tumor antigens TOMM34 and RNF43 in samples extracted from paraffin-embedded specimens of colorectal cancer.  Oncol Lett  2017  14:2281-2287  PubMed ID: 28789449   DOI: 10.3892/ol.2017.6412
10498  Inaguma S, Lasota J, Felisiak-Golabek A, Kowalik A, Wang Z, Zieba S, Kalisz J, Ikeda H, Miettinen M.  Histopathological and genotypic characterization of metastatic colorectal carcinoma with PD-L1 (CD274)-expression: Possible roles of tumour micro environmental factors for CD274 expression.  J Pathol Clin Res  2017  3:268-278  PubMed ID: 29085667   DOI: 10.1002/cjp2.81
14055  Morishita S, Suzuki T, Niwa Y, Dohmae N, Simizu S.  Dpy-19 like 3-mediated C-mannosylation and expression levels of RPE-spondin in human tumor cell lines  Oncol Lett  2017  14(2):2537-2544  PubMed ID: 28781692   DOI: 10.3892/ol.2017.6465
9508  Kaku Y, Tsuchiya A, Shimizu T, Tanaka A, Nishizaki T.  HUHS1015 Suppresses Colonic Cancer Growth by Inducing Necrosis and Apoptosis in Association with Mitochondrial Damage.  Anticancer Res.  2016  36:39-48  PubMed ID: 26722026  
9823  Novotny CJ, Pollari S, Park JH, Lemmon MA, Shen W, Shokat KM.  Overcoming resistance to HER2 inhibitors through state-specific kinase binding.  Nat. Chem. Biol.  2016  12:923-930  PubMed ID: 27595329   DOI: 10.1038/nchembio.2171
15077  Kloth M, Ruesseler V, Engel C, Koenig K, Peifer M, Mariotti E, Kuenstlinger H, Florin A, Rommerscheidt-Fuss U, Koitzsch U, Wodtke C, Ueckeroth F, Holzapfel S, Aretz S, Propping P, Loeffler M, Merkelbach-Bruse S, Odenthal M, Friedrichs N, Heukamp LC, Zander T, Buettner R.  Activating ERBB2/HER2 mutations indicate susceptibility to pan-HER inhibitors in Lynch and Lynch-like colorectal cancer  Gut  2016  65(8):1296-305  PubMed ID: 26001389   DOI: 10.1136/gutjnl-2014-309026
7334  Ito H, Hasegawa K, Hasegawa Y, Nishimaki T, Hosomichi K, Kimura S, Ohba M, Yao H, Onimaru M, Inoue I, Inoue H.  Silver Nanoscale Hexagonal Column Chips for Detecting Cell-free DNA and Circulating Nucleosomes in Cancer Patients.  Sci Rep  2015  5:10455  PubMed ID: 25994878   DOI: 10.1038/srep10455
7414  Kaku Y, Tsuchiya A, Kanno T, Nishizaki T.  Irinotecan induces cell cycle arrest, but not apoptosis or necrosis, in Caco-2 and CW2 colorectal cancer cell lines.  Pharmacology  2015  95:154-9  PubMed ID: 25833236   DOI: 10.1159/000381029
10158  Takuma Yoshinaga, Takamasa Shigemitsu, Hiroto Nishimata, Masaki Kitazono, Emiko Hori, Ayako Tomiyoshi, Takayuki Takei, Masahiro Yoshida  Angiopoietin-like Protein 2 as a Potential Biomarker for Colorectal Cancer  Mol Clin Oncol  2015  3(5):1080-1084  PubMed ID: 26623054   DOI: 10.3892/mco.2015.577
15087  Kauko O, Laajala TD, Jumppanen M, Hintsanen P, Suni V, Haapaniemi P, Corthals G, Aittokallio T, Westermarck J, Imanishi SY.  Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling  Sci Rep  2015  5:13099  PubMed ID: 26278961   DOI: 10.1038/srep13099
10244  Kazuhiro Ishiguro, Takafumi Ando, Osamu Maeda, Osamu Watanabe, Hidemi Goto  Suppressive Action of Acetate on interleukin-8 Production via Tubulin-α Acetylation  Immunol Cell Biol  2014  92(7):624-30  PubMed ID: 24777307   DOI: 10.1038/icb.2014.31
15079  Ålgars A, Avoranta T, Österlund P, Lintunen M, Sundström J, Jokilehto T, Ristimäki A, Ristamäki R, Carpén O.  Heterogeneous EGFR gene copy number increase is common in colorectal cancer and defines response to anti-EGFR therapy  PLoS One  2014  9(6):e99590  PubMed ID: 24940619   DOI: 10.1371/journal.pone.0099590
15086  Katase N, Lefeuvre M, Tsujigiwa H, Fujii M, Ito S, Tamamura R, Buery RR, Gunduz M, Nagatsuka H.  Knockdown of Dkk-3 decreases cancer cell migration and invasion independently of the Wnt pathways in oral squamous cell carcinoma‑derived cells  Oncol Rep  2013  29(4):1349-55  PubMed ID: 23354949   DOI: 10.3892/or.2013.2251
6316  Miyaki Y, Suzuki K, Koizumi K, Kato T, Saito M, Kamiyama H, Maeda T, Shibata K, Shiya N, Konishi F.  Identification of a potent epigenetic biomarker for resistance to camptothecin and poor outcome to irinotecan-based chemotherapy in colon cancer.  Int J Oncol  2012  40(1):217-26  PubMed ID: 21901246   DOI: 10.3892/ijo.2011.1189
6317  Koizumi K, Alonso S, Miyaki Y, Okada S, Ogura H, Shiiya N, Konishi F, Taya T, Perucho M, Suzuki K.  Array-based identification of common DNA methylation alterations in ulcerative colitis.  Int J Oncol  2012  40(4):983-94  PubMed ID: 22159500   DOI: 10.3892/ijo.2011.1283
15078  Hanada N, Takahata T, Zhou Q, Ye X, Sun R, Itoh J, Ishiguro A, Kijima H, Mimura J, Itoh K, Fukuda S, Saijo Y.  Methylation of the KEAP1 gene promoter region in human colorectal cancer  BMC Cancer  2012  12:66  PubMed ID: 22325485   DOI: 10.1186/1471-2407-12-66
15085  Hasegawa M, Ishiguro K, Ando T, Goto H.  Geranylgeranylacetone attenuates cisplatin-induced reductions in cell viability by suppressing the elevation of intracellular p53 content without heat shock protein induction  Nagoya J Med Sci  2012  74(1-2):123-31  PubMed ID: 22515118  
7823  Matsuo T, Nishizuka SS, Ishida K, Iwaya T, Ikeda M, Wakabayashi G.  Analysis of the anti-tumor effect of cetuximab using protein kinetics and mouse xenograft models.  BMC Res Notes  2011  4:140  PubMed ID: 21554739   DOI: 10.1186/1756-0500-4-140
15083  Noriko Yoshikawa, Arisa Nishiuchi, Erika Kubo, Yu Yamaguchi, Masaru Kunitomo, Satomi Kagota, Kazumasa Shinozuka, Kazuki Nakamura  Cordyceps sinensis Acts as an Adenosine A3 Receptor Agonist on Mouse Melanoma and Lung Carcinoma Cells, and Human Fibrosarcoma and Colon Carcinoma Cells  Pharmacology & Pharmacy  2011  2:266-270    DOI: 10.4236/pp.2011.24034
8475  Saito M, Yaguchi T, Yasuda Y, Nakano T, Nishizaki T.  Adenosine suppresses CW2 human colonic cancer growth by inducing apoptosis via A(1) adenosine receptors.  Cancer Lett.  2010  290:211-5  PubMed ID: 19822392   DOI: 10.1016/j.canlet.2009.09.011
5882  Shichiri M, Fukai N, Kono Y, Tanaka Y.  Rifampicin as an oral angiogenesis inhibitor targeting hepatic cancers.  Cancer Res  2009  69(11):4760-8  PubMed ID: 19458074   DOI: 10.1158/0008-5472.CAN-08-3417
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
18148  Ishiguro K, Ando T, Goto H.  Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells  Biotechniques  2008  45(4):465, 467-8  PubMed ID: 18855774   DOI: 10.2144/000112912
9428  Chirila T, Barnard Z, Zainuddin, Harkin DG, Schwab IR, Hirst L.  Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders.  Tissue Eng Part A  2008  14(7):1203-11  PubMed ID: 18380593   DOI: 10.1089/ten.tea.2007.0224
15084  Maeda O, Kondo M, Fujita T, Usami N, Fukui T, Shimokata K, Ando T, Goto H, Sekido Y.  Enhancement of GLI1-transcriptional activity by beta-catenin in human cancer cells  Oncol Rep  2006  16(1):91-6  PubMed ID: 16786128  
8943  Sun L, Fu BB, Liu DG.  Systemic delivery of full-length C/EBP beta/liposome complex suppresses growth of human colon cancer in nude mice.  Cell Res.  2005  15:770-6  PubMed ID: 16246266   DOI: 10.1038/sj.cr.7290346
18147  Hara M, Adachi S, Higuchi A.  Enhanced production of carcinoembryonic antigen by CW-2 cells cultured on polymeric membranes immobilized with extracellular matrix proteins  J Biomater Sci Polym Ed  2003  14(2):139-55  PubMed ID: 12661665   DOI: 10.1163/156856203321142588
3762  Kataoka H, Itoh H, Uchino H, Hamasuna R, Kitamura N, Nabeshima K, Koono M.  Conserved expression of hepatocyte growth factor activator inhibitor type-2/placental bikunin in human colorectal carcinomas.  Cancer Lett  2000  148(2):127-34  PubMed ID: 10695988   DOI: 10.1016/s0304-3835(99)00322-5
18149  Higuchi A, Uchiyama S, Demura M, Asakura T, Cho CS, Akaike T, Takarada H, Hara M.  Enhanced CEA production associated with aspirin in a culture of CW-2 cells on some polymeric films  Cytotechnology  1999  31(3):233-42  PubMed ID: 19003147   DOI: 10.1023/A:1008030730814
18150  Kono T, Watanabe M, Koyama K, Kishimoto T, Fukushima S, Sugimura T, Wakabayashi K.  Cytotoxic activity of pierisin, from the cabbage butterfly, Pieris rapae, in various human cancer cell lines  Cancer Lett  1999  137(1):75-81  PubMed ID: 10376796   DOI: 10.1016/s0304-3835(98)00346-2
18151  Ueda T, Furui J, Komuta K, Yamaguchi J, Yamamoto M, Furukawa K, Kanematsu T.  Detection of carcinoembryonic antigen mRNA in the mesenteric vein of patients with resectable colorectal cancer  Surg Today  1998  28(7):701-6  PubMed ID: 9697262   DOI: 10.1007/BF02484615
3754  Nabeshima K, Shimao Y, Inoue T, Itoh H, Kataoka H, Koono M.  Hepatocyte growth factor/scatter factor induces not only scattering but also cohort migration of human colorectal-adenocarcinoma cells.  Int J Cancer  1998  78(6):750-9  PubMed ID: 9833769   DOI: 10.1002/(sici)1097-0215(19981209)78:6<750::aid-ijc13>3.0.co;2-#
3755  Kataoka H, Uchino H, Denda K, Kitamura N, Itoh H, Tsubouchi H, Nabeshima K, Koono M.  Evaluation of hepatocyte growth factor activator inhibitor expression in normal and malignant colonic mucosa.  Cancer Lett  1998  19;128(2):219-27  PubMed ID: 9683286   DOI: 10.1016/s0304-3835(98)00067-6
3752  Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A, Koono M.  Analysis of tissue factor and tissue factor pathway inhibitor expression in human colorectal carcinoma cell lines and metastatic sublines to the liver.  Int J Cancer  1997  72(5):878-84  PubMed ID: 9311608   DOI: 10.1002/(sici)1097-0215(19970904)72:5<878::aid-ijc27>3.0.co;2-3

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利用者成果(日)
2197  高田学  大腸癌新鮮切除標本をもちいたpovidone-iodine液による化学的腸管洗浄法の検討  日本消化器外科学会雑誌  1997  30:1739-1746    DOI: 10.5833/jjgs.30.1739



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