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Cell No. : Cell Name
RCB0778 : CW-2  update : 2021/12/21
CommentColon carcinoma. CEA-positive, nude mouse-transplantable.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mitsuhashi, Maki
Originator Mitsuhashi, Maki
Year of deposit 1992
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 55 years
Tissue colon
Classification cancer
Year of origin 1990
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1151
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-92(50) : 44(1),45(5),46(42),47(1),92(1)
STR(human) OK
deposit info
lot info
Reference information Reference 0
User's Publication 39

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User's Publication
20203  Kast F, Schwill M, Stüber JC, Pfundstein S, Nagy-Davidescu G, Rodríguez JMM, Seehusen F, Richter CP, Honegger A, Hartmann KP, Weber TG, Kroener F, Ernst P, Piehler J, Plückthun A.  Engineering an anti-HER2 biparatopic antibody with a multimodal mechanism of action.  Nat Commun  2021  12(1):3790  PubMed ID: 34145240
15080  Hirashita Y, Tsukamoto Y, Kudo Y, Kakisako D, Kurogi S, Hijiya N, Nakada C, Uchida T, Hirashita T, Hiratsuka T, Akagi T, Ueda Y, Shiroshita H, Etoh T, Mizukami K, Honda K, Okimoto T, Kodama M, Inomata M, Moriyama M, Murakami K.  Early response in phosphorylation of ribosomal protein S6 is associated with sensitivity to trametinib in colorectal cancer cells  Lab Invest  2021  101(8):1036-1047  PubMed ID: 33911189
15088  Hanker AB, Brown BP, Meiler J, Marín A, Jayanthan HS, Ye D, Lin CC, Akamatsu H, Lee KM, Chatterjee S, Sudhan DR, Servetto A, Brewer MR, Koch JP, Sheehan JH, He J, Lalani AS, Arteaga CL.  Co-occurring gain-of-function mutations in HER2 and HER3 modulate HER2/HER3 activation, oncogenesis, and HER2 inhibitor sensitivity  Cancer Cell  2021  39(8):1099-1114.e8  PubMed ID: 34171264
9913  Satoshi Inoue, Takumi Tsunoda, Miho Riku, Hideaki Ito, Akihito Inoko, Hideki Murakami, Masahide Ebi, Naotaka Ogasawara, Ira Pastan, Kunio Kasugai, Kenji Kasai, Hiroshi Ikeda, Shingo Inaguma  Diffuse Mesothelin Expression Leads to Worse Prognosis Through Enhanced Cellular Proliferation in Colorectal Cancer  Oncol Lett  2020  19(3):1741-1750  PubMed ID: 32194667
11927  Yang J, Isaji T, Zhang G, Qi F, Duan C, Fukuda T, Gu J.  EpCAM associates with integrin and regulates cell adhesion in cancer cells.  Biochem. Biophys. Res. Commun.  2020    PubMed ID: 31806375
13234  Nishimoto A, Takemoto Y, Saito T, Kurazumi H, Tanaka T, Harada E, Shirasawa B, Hamano K.  Nuclear β-catenin expression is positively regulated by JAB1 in human colorectal cancer cells.  Biochem Biophys Res Commun  2020    PubMed ID: 32977947
13325  Fernando TM, Piskol R, Bainer R, Sokol ES, Trabucco SE, Zhang Q, Trinh H, Maund S, Kschonsak M, Chaudhuri S, Modrusan Z, Januario T, Yauch RL.  Functional characterization of SMARCA4 variants identified by targeted exome-sequencing of 131,668 cancer patients.  Nat Commun  2020    PubMed ID: 33144586
11342  Washio I, Nakanishi T, Ishiguro N, Bister B, Tamai I.  Effect of endogenous multidrug resistance 1 and P-glycoprotein expression on anticancer drug resistance in colon cancer cell lines.  Biopharm Drug Dispos  2019    PubMed ID: 30556139
11364  Kimura T, Tsukada A, Fukutomi T, Ichida K, Ohtsuki S, Sakurai H.  Urate Transport via Paracellular Route across Epithelial Cells.  Biol. Pharm. Bull.  2019    PubMed ID: 30606989
11553  Wu Z, Ichinose T, Naoe Y, Matsumura S, Villalobos IB, Eissa IR, Yamada S, Miyajima N, Morimoto D, Mukoyama N, Nishikawa Y, Koide Y, Kodera Y, Tanaka M, Kasuya H.  Combination of Cetuximab and Oncolytic Virus Canerpaturev Synergistically Inhibits Human Colorectal Cancer Growth.  Mol Ther Oncolytics  2019    PubMed ID: 31193737
11832  Robichaux JP, Elamin YY, Vijayan RSK, Nilsson MB, Hu L, He J, Zhang F, Pisegna M, Poteete A, Sun H, Li S, Chen T, Han H, Negrao MV, Ahnert JR, Diao L, Wang J, Le X, Meric-Bernstam F, Routbort M, Roeck B, Yang Z, Raymond VM, Lanman RB, Frampton GM, Miller VA, Schrock AB, Albacker LA, Wong KK, Cross JB, Heymach JV.  Pan-Cancer Landscape and Analysis of ERBB2 Mutations Identifies Poziotinib as a Clinically Active Inhibitor and Enhancer of T-DM1 Activity.  Cancer Cell  2019    PubMed ID: 31588020
10398  Matsushita N, Yamamoto S, Inoue Y, Aruga A, Yamamoto M.  RT-qPCR analysis of the tumor antigens TOMM34 and RNF43 in samples extracted from paraffin-embedded specimens of colorectal cancer.  Oncol Lett  2017  14:2281-2287  PubMed ID: 28789449
10498  Inaguma S, Lasota J, Felisiak-Golabek A, Kowalik A, Wang Z, Zieba S, Kalisz J, Ikeda H, Miettinen M.  Histopathological and genotypic characterization of metastatic colorectal carcinoma with PD-L1 (CD274)-expression: Possible roles of tumour micro environmental factors for CD274 expression.  J Pathol Clin Res  2017  3:268-278  PubMed ID: 29085667
9508  Kaku Y, Tsuchiya A, Shimizu T, Tanaka A, Nishizaki T.  HUHS1015 Suppresses Colonic Cancer Growth by Inducing Necrosis and Apoptosis in Association with Mitochondrial Damage.  Anticancer Res.  2016  36:39-48  PubMed ID: 26722026
9823  Novotny CJ, Pollari S, Park JH, Lemmon MA, Shen W, Shokat KM.  Overcoming resistance to HER2 inhibitors through state-specific kinase binding.  Nat. Chem. Biol.  2016  12:923-930  PubMed ID: 27595329
15077  Kloth M, Ruesseler V, Engel C, Koenig K, Peifer M, Mariotti E, Kuenstlinger H, Florin A, Rommerscheidt-Fuss U, Koitzsch U, Wodtke C, Ueckeroth F, Holzapfel S, Aretz S, Propping P, Loeffler M, Merkelbach-Bruse S, Odenthal M, Friedrichs N, Heukamp LC, Zander T, Buettner R.  Activating ERBB2/HER2 mutations indicate susceptibility to pan-HER inhibitors in Lynch and Lynch-like colorectal cancer  Gut  2016  65(8):1296-305  PubMed ID: 26001389
7334  Ito H, Hasegawa K, Hasegawa Y, Nishimaki T, Hosomichi K, Kimura S, Ohba M, Yao H, Onimaru M, Inoue I, Inoue H.  Silver Nanoscale Hexagonal Column Chips for Detecting Cell-free DNA and Circulating Nucleosomes in Cancer Patients.  Sci Rep  2015  5:10455  PubMed ID: 25994878
7414  Kaku Y, Tsuchiya A, Kanno T, Nishizaki T.  Irinotecan induces cell cycle arrest, but not apoptosis or necrosis, in Caco-2 and CW2 colorectal cancer cell lines.  Pharmacology  2015  95:154-9  PubMed ID: 25833236
10158  Takuma Yoshinaga, Takamasa Shigemitsu, Hiroto Nishimata, Masaki Kitazono, Emiko Hori, Ayako Tomiyoshi, Takayuki Takei, Masahiro Yoshida  Angiopoietin-like Protein 2 as a Potential Biomarker for Colorectal Cancer  Mol Clin Oncol  2015  3(5):1080-1084  PubMed ID: 26623054
15087  Kauko O, Laajala TD, Jumppanen M, Hintsanen P, Suni V, Haapaniemi P, Corthals G, Aittokallio T, Westermarck J, Imanishi SY.  Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling  Sci Rep  2015  5:13099  PubMed ID: 26278961
10244  Kazuhiro Ishiguro, Takafumi Ando, Osamu Maeda, Osamu Watanabe, Hidemi Goto  Suppressive Action of Acetate on interleukin-8 Production via Tubulin-α Acetylation  Immunol Cell Biol  2014  92(7):624-30  PubMed ID: 24777307
15079  Ålgars A, Avoranta T, Österlund P, Lintunen M, Sundström J, Jokilehto T, Ristimäki A, Ristamäki R, Carpén O.  Heterogeneous EGFR gene copy number increase is common in colorectal cancer and defines response to anti-EGFR therapy  PLoS One  2014  9(6):e99590  PubMed ID: 4940619
15086  Katase N, Lefeuvre M, Tsujigiwa H, Fujii M, Ito S, Tamamura R, Buery RR, Gunduz M, Nagatsuka H.  Knockdown of Dkk-3 decreases cancer cell migration and invasion independently of the Wnt pathways in oral squamous cell carcinoma‑derived cells  Oncol Rep  2013  29(4):1349-55  PubMed ID: 23354949
6316  Miyaki Y, Suzuki K, Koizumi K, Kato T, Saito M, Kamiyama H, Maeda T, Shibata K, Shiya N, Konishi F.  Identification of a potent epigenetic biomarker for resistance to camptothecin and poor outcome to irinotecan-based chemotherapy in colon cancer.  Int J Oncol  2012  40(1):217-26  PubMed ID: 21901246
6317  Koizumi K, Alonso S, Miyaki Y, Okada S, Ogura H, Shiiya N, Konishi F, Taya T, Perucho M, Suzuki K.  Array-based identification of common DNA methylation alterations in ulcerative colitis.  Int J Oncol  2012  40(4):983-94  PubMed ID: 22159500
15078  Hanada N, Takahata T, Zhou Q, Ye X, Sun R, Itoh J, Ishiguro A, Kijima H, Mimura J, Itoh K, Fukuda S, Saijo Y.  Methylation of the KEAP1 gene promoter region in human colorectal cancer  BMC Cancer  2012  12:66  PubMed ID: 22325485
15085  Hasegawa M, Ishiguro K, Ando T, Goto H.  Geranylgeranylacetone attenuates cisplatin-induced reductions in cell viability by suppressing the elevation of intracellular p53 content without heat shock protein induction  Nagoya J Med Sci  2012  74(1-2):123-31  PubMed ID: 22515118
7823  Matsuo T, Nishizuka SS, Ishida K, Iwaya T, Ikeda M, Wakabayashi G.  Analysis of the anti-tumor effect of cetuximab using protein kinetics and mouse xenograft models.  BMC Res Notes  2011  4:140  PubMed ID: 21554739
15083  Noriko Yoshikawa, Arisa Nishiuchi, Erika Kubo, Yu Yamaguchi, Masaru Kunitomo, Satomi Kagota, Kazumasa Shinozuka, Kazuki Nakamura  Cordyceps sinensis Acts as an Adenosine A3 Receptor Agonist on Mouse Melanoma and Lung Carcinoma Cells, and Human Fibrosarcoma and Colon Carcinoma Cells  Pharmacology & Pharmacy  2011  2:266-270 
8475  Saito M, Yaguchi T, Yasuda Y, Nakano T, Nishizaki T.  Adenosine suppresses CW2 human colonic cancer growth by inducing apoptosis via A(1) adenosine receptors.  Cancer Lett.  2010  290:211-5  PubMed ID: 19822392
5882  Shichiri M, Fukai N, Kono Y, Tanaka Y.  Rifampicin as an oral angiogenesis inhibitor targeting hepatic cancers.  Cancer Res  2009  69(11):4760-8  PubMed ID: 19458074
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
9428  Chirila T, Barnard Z, Zainuddin, Harkin DG, Schwab IR, Hirst L.  Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders.  Tissue Eng Part A  2008  14(7):1203-11  PubMed ID: 18380593
15084  Maeda O, Kondo M, Fujita T, Usami N, Fukui T, Shimokata K, Ando T, Goto H, Sekido Y.  Enhancement of GLI1-transcriptional activity by beta-catenin in human cancer cells  Oncol Rep  2006  16(1):91-6  PubMed ID: 16786128
8943  Sun L, Fu BB, Liu DG.  Systemic delivery of full-length C/EBP beta/liposome complex suppresses growth of human colon cancer in nude mice.  Cell Res.  2005  15:770-6  PubMed ID: 16246266
3762  Kataoka H, Itoh H, Uchino H, Hamasuna R, Kitamura N, Nabeshima K, Koono M.  Conserved expression of hepatocyte growth factor activator inhibitor type-2/placental bikunin in human colorectal carcinomas.  Cancer Lett  2000  148(2):127-34  PubMed ID: 10695988
3754  Nabeshima K, Shimao Y, Inoue T, Itoh H, Kataoka H, Koono M.  Hepatocyte growth factor/scatter factor induces not only scattering but also cohort migration of human colorectal-adenocarcinoma cells.  Int J Cancer  1998  78(6):750-9  PubMed ID: 9833769
3755  Kataoka H, Uchino H, Denda K, Kitamura N, Itoh H, Tsubouchi H, Nabeshima K, Koono M.  Evaluation of hepatocyte growth factor activator inhibitor expression in normal and malignant colonic mucosa.  Cancer Lett  1998  19;128(2):219-27  PubMed ID: 9683286
3752  Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A, Koono M.  Analysis of tissue factor and tissue factor pathway inhibitor expression in human colorectal carcinoma cell lines and metastatic sublines to the liver.  Int J Cancer  1997  72(5):878-84  PubMed ID: 9311608

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