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Cell No. : Cell Name
RCB0777 : HMV-II  update : 2022/03/24
CommentMelanin producing. Originated from the same site of HMV-I.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kasuga, Tsutomu
Originator Kasuga, Tsutomu
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 65 years
Tissue vagina
Disease name melanoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1282
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, MPI, PepB
Chromosome mode 61-134(50) : /63(7),64(12),65(16),66(5),66(5),67(2)/
STR(human) OK
deposit info
lot info
Reference information Reference 6
User's Publication 25

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1535  Nakamura T, Seki S, Matsubara O, Ito S, Kasuga T.  Specific incorporation of 4-S-cysteinylphenol into human melanoma cells.  J Invest Dermatol  1988  90(5):725-8  PubMed ID: 3361142   DOI: 10.1111/1523-1747.ep12560926
1534  Inoue S, Ito S, Imai Y, Kasuga T, Fujita K.  Growth inhibition of melanoma cells by N-protected dopa derivatives.  Biochem Pharmacol  1987  36(20):3537-40  PubMed ID: 3118879   DOI: 10.1016/0006-2952(87)90340-6
1536  Yamada I, Seki S, Matsubara O, Ito S, Suzuki S, Kasuga T.  The cytotoxicity of cysteinylcatechols and related compounds to human melanoma cells in vitro.  J Invest Dermatol  1987  88(5):538-40  PubMed ID: 3572026   DOI: 10.1111/1523-1747.ep12470098
968  Hasumi K, Sakamoto G, Sugano H, Kasuga T, Masubuchi K  Primary malignant melanoma of the vagina: study of four autopsy cases with ultrastructural findings.  Cancer  1978  42:2675-86  PubMed ID: 365316   DOI: 10.1002/1097-0142(197812)42:6<2675::aid-cncr2820420624>;2-g
4817  Inada T, Kasuga T, Nojiri I, Hiraoka T, Furuse T.  Comparative study on radiosensitivities of cultured cell lines derived from several human tumors under hypoxic condition.  Gan  1977  68(3):357-62  PubMed ID: 913957  
4816  Inada T, Kasuga T, Nojiri I, Furuse T, Hiraoka T.  Linear energy transfer-dependent radiosensitivity of Burkitt lymphoma cells, with special references to human melanoma HMV, HeLa-S3, and L5178Y cells.  Gan  1976  67(3):399-402  PubMed ID: 826441  

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User's Publication
20835  Mizuta K, Matsubara T, Goto A, Addison WN, Nakatomi M, Matsuo K, Tada-Shigeyama Y, Yaginuma T, Honda H, Yoshioka I, Kokabu S.  Plectin promotes tumor formation by B16 mouse melanoma cells via regulation of Rous sarcoma oncogene activity.  BMC Cancer  2022  22(1):936  PubMed ID: 36038818   DOI: 10.1186/s12885-022-10033-4
15286  Yuki Ohno, Shiori Kondo, Kiho Tajima, Toshiyuki Shibata, Tomohiro Itoh  Effect of Phlorotannins Isolated From Eisenia bicyclis on Melanogenesis in Mouse B16 Melanoma Cells  Natural Product Communications  2021  16:1934578X2110192    DOI: 10.1177/1934578x211019264
12992  Taguchi N, Hata T, Kamiya E, Homma T, Kobayashi A, Aoki H, Kunisada T.  Eriodictyon angustifolium extract, but not Eriodictyon californicum extract, reduces human hair greying.  Int J Cosmet Sci  2020    PubMed ID: 32324292   DOI: 10.1111/ics.12620
6893  Ogawa M, Yaginuma T, Nakatomi C, Nakajima T, Tada-Shigeyama Y, Addison WN, Urata M, Matsubara T, Watanabe K, Matsuo K, Sato T, Honda H, Hikiji H, Watanabe S, Kokabu S.  Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity.  Oncotarget  2019  10(3):404-414  PubMed ID: 30719233   DOI: 10.18632/oncotarget.26552
4233  Homma T, Kageyama S, Nishikawa A, Nagata K.  Melanosome degradation in epidermal keratinocytes related to lysosomal protease cathepsin V.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 29654760   DOI: 10.1016/j.bbrc.2018.04.070
9516  Samatiwat P, Takeda K, Satarug S, Ohba K, Kukongviriyapan V, Shibahara S.  Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling.  Biochem. Biophys. Res. Commun.  2016  470:144-9  PubMed ID: 26773496   DOI: 10.1016/j.bbrc.2016.01.010
7161  Ohba K, Takeda K, Yamamoto H, Shibahara S.  Microphthalmia-associated transcription factor is expressed in projection neurons of the mouse olfactory bulb.  Genes Cells  2015  20:1088-102  PubMed ID: 26522736   DOI: 10.1111/gtc.12312
14531  Hisahiro Kai, Koji Matsuno  Assessment of the Effect of Arbutin Isomers and Kojic Acid on Melanin Production, Tyrosinase Activity, and Tyrosinase Expression in B16-4A5 and HMV-II Melanoma Cells  Planta Medica Letters  2015  2:e39-e41    DOI: 10.1055/s-0035-1557833
15908  Takekoshi S, Nagata H, Kitatani K.  Stimulation of melanogenesis by nordihydroguaiaretic Acid in human melanoma cells  Acta Histochem Cytochem  2014  47(5):203-10  PubMed ID: 25861126   DOI: 10.1267/ahc.14033
16001  Yanagishita T, Yajima I, Kumasaka M, Kawamoto Y, Tsuzuki T, Matsumoto Y, Watanabe D, Kato M.  Actin-binding protein, Espin: a novel metastatic regulator for melanoma  Mol Cancer Res  2014  12(3):440-6  PubMed ID: 24337068   DOI: 10.1158/1541-7786.MCR-13-0468-T
17131  Takekoshi S, Nagata H, Kitatani K.  Flavonoids enhance melanogenesis in human melanoma cells  Tokai J Exp Clin Med  2014  39(3):116-21  PubMed ID: 25248426  
6320  Shono M, Yoshioka R, Chatani Y, Hirai Y.  Ectopic expression of syntaxin3 affects behaviors of B16 melanoma by controlling actin dynamics.  Cell Struct Funct  2013  38(1):97-107  PubMed ID: 23546178   DOI: 10.1247/csf.12032
16017  Watanabe M, Umezawa K, Higashihara M, Horie R.  Combined inhibition of NF-κB and Bcl-2 triggers synergistic reduction of viability and induces apoptosis in melanoma cells  Oncol Res  2013  21(4):173-80  PubMed ID: 24762223   DOI: 10.3727/096504014X13887748696707
17157  Watanuki Y, Takayasu S, Kageyama K, Iwasaki Y, Sakihara S, Terui K, Nigawara T, Suda T.  Involvement of Nurr-1/Nur77 in corticotropin-releasing factor/urocortin1-induced tyrosinase-related protein 1 gene transcription in human melanoma HMV-II cells  Mol Cell Endocrinol  2013  370(1-2):42-51  PubMed ID: 23416839   DOI: 10.1016/j.mce.2013.01.022
7610  Thang ND, Yajima I, Nakagawa K, Tsuzuki T, Kumasaka MY, Ohgami N, Ly TB, Iwamoto T, Watanabe D, Kato M.  A novel hairless mouse model for malignant melanoma.  J. Dermatol. Sci.  2012  65:207-12  PubMed ID: 22169325   DOI: 10.1016/j.jdermsci.2011.10.010
7976  Ogi T, Higa M, Maruyama S.  Melanin synthesis inhibitors from Balanophora fungosa.  J. Agric. Food Chem.  2011  59:1109-14  PubMed ID: 21265535   DOI: 10.1021/jf1035542
8083  Kim KS, Choi HW, Yoon HE, Kim IY.  Reactive oxygen species generated by NADPH oxidase 2 and 4 are required for chondrogenic differentiation.  J. Biol. Chem.  2010  285:40294-302  PubMed ID: 20952384   DOI: 10.1074/jbc.M110.126821
13479  Matsumoto Y, Iwakawa M, Furusawa Y, Ishikawa K, Aoki M, Imadome K, Matsumoto I, Tsujii H, Ando K, Imai T.  Gene expression analysis in human malignant melanoma cell lines exposed to carbon beams  Int J Radiat Biol  2008  84(4):299-314  PubMed ID: 18386195   DOI: 10.1080/09553000801953334
3374  Yanagisawa, Ayano, Endo, Chisato, Okawa, Katsuya, Shitara, Shingo, Kugoh, Hiroyuki, Kakitani, Makoto, Oshimura, Mitsuo, Tomizuka, Kazuma  Generation of chromosome-specific monoclonal antibodies using in vitro-differentiated transchromosomic mouse embryonic stem cells.  Stem Cells  2005  23:1479-88  PubMed ID: 16081665   DOI: 10.1634/stemcells.2004-0369
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014   DOI: 10.1111/j.1349-7006.2002.tb02174.x
13672  Takeda K, Yasumoto Ki, Kawaguchi N, Udono T, Watanabe Ki, Saito H, Takahashi K, Noda M, Shibahara S.  Mitf-D, a newly identified isoform, expressed in the retinal pigment epithelium and monocyte-lineage cells affected by Mitf mutations  Biochim Biophys Acta  2002  1574(1):15-23  PubMed ID: 11955610   DOI: 10.1016/s0167-4781(01)00339-6
15611  Udono T, Takahashi K, Yasumoto K, Yoshizawa M, Takeda K, Abe T, Tamai M, Shibahara S.  Expression of tyrosinase-related protein 2/DOPAchrome tautomerase in the retinoblastoma  Exp Eye Res  2001  72(3):225-34  PubMed ID: 11180971   DOI: 10.1006/exer.2000.0948
12280  K Yasumoto , K Yokoyama, K Shibata, Y Tomita, S Shibahara  Microphthalmia-associated transcription factor as a regulator for melanocyte-specific transcription of the human tyrosinase gene  Mol Cell Biol  1995  15(3):1833   PubMed ID: 7862173   DOI: 10.1128/mcb.15.3.1833
12279  K Yasumoto, K Yokoyama, K Shibata, Y Tomita, S Shibahara  Microphthalmia-associated transcription factor as a regulator for melanocyte-specific transcription of the human tyrosinase gene  Mol Cell Biol  1994  14(12):8058-70  PubMed ID: 7969144   DOI: 10.1128/mcb.14.12.8058
1619  Suzuki H, Takahashi K, Yasumoto K, Shibahara S.  Activation of the tyrosinase gene promoter by neurofibromin  Biochem Biophys Res Commun  1994  205(3):1984-91  PubMed ID: 7811291   DOI: 10.1006/bbrc.1994.2903

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