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Cell No. : Cell Name
RCB0777 : HMV-II  update : 2021/02/26
CommentMelanin producing. Originated from the same site of HMV-I.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Kasuga, Tsutomu
Originator Kasuga, Tsutomu
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 65 years
Tissue vagina
Disease name melanoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP, MPI, PepB
Chromosome mode 61-134(50) : /63(7),64(12),65(16),66(5),66(5),67(2)/
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lot info
Reference information Reference 6
User's Publication 13

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1535  Nakamura T, Seki S, Matsubara O, Ito S, Kasuga T.  Specific incorporation of 4-S-cysteinylphenol into human melanoma cells.  J Invest Dermatol  1988  90(5):725-8  PubMed ID: 3361142
1534  Inoue S, Ito S, Imai Y, Kasuga T, Fujita K.  Growth inhibition of melanoma cells by N-protected dopa derivatives.  Biochem Pharmacol  1987  36(20):3537-40  PubMed ID: 3118879
1536  Yamada I, Seki S, Matsubara O, Ito S, Suzuki S, Kasuga T.  The cytotoxicity of cysteinylcatechols and related compounds to human melanoma cells in vitro.  J Invest Dermatol  1987  88(5):538-40  PubMed ID: 3572026
968  Hasumi K, Sakamoto G, Sugano H, Kasuga T, Masubuchi K  Primary malignant melanoma of the vagina: study of four autopsy cases with ultrastructural findings.  Cancer  1978  42:2675-86  PubMed ID: 365316
4817  Inada T, Kasuga T, Nojiri I, Hiraoka T, Furuse T.  Comparative study on radiosensitivities of cultured cell lines derived from several human tumors under hypoxic condition.  Gan  1977  68(3):357-62  PubMed ID: 913957
4816  Inada T, Kasuga T, Nojiri I, Furuse T, Hiraoka T.  Linear energy transfer-dependent radiosensitivity of Burkitt lymphoma cells, with special references to human melanoma HMV, HeLa-S3, and L5178Y cells.  Gan  1976  67(3):399-402  PubMed ID: 826441

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User's Publication
12992  Taguchi N, Hata T, Kamiya E, Homma T, Kobayashi A, Aoki H, Kunisada T.  Eriodictyon angustifolium extract, but not Eriodictyon californicum extract, reduces human hair greying.  Int J Cosmet Sci  2020    PubMed ID: 32324292
4233  Homma T, Kageyama S, Nishikawa A, Nagata K.  Melanosome degradation in epidermal keratinocytes related to lysosomal protease cathepsin V.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 29654760
9516  Samatiwat P, Takeda K, Satarug S, Ohba K, Kukongviriyapan V, Shibahara S.  Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling.  Biochem. Biophys. Res. Commun.  2016  470:144-9  PubMed ID: 26773496
7161  Ohba K, Takeda K, Yamamoto H, Shibahara S.  Microphthalmia-associated transcription factor is expressed in projection neurons of the mouse olfactory bulb.  Genes Cells  2015  20:1088-102  PubMed ID: 26522736
6320  Shono M, Yoshioka R, Chatani Y, Hirai Y.  Ectopic expression of syntaxin3 affects behaviors of B16 melanoma by controlling actin dynamics.  Cell Struct Funct  2013  38(1):97-107  PubMed ID: 23546178
7610  Thang ND, Yajima I, Nakagawa K, Tsuzuki T, Kumasaka MY, Ohgami N, Ly TB, Iwamoto T, Watanabe D, Kato M.  A novel hairless mouse model for malignant melanoma.  J. Dermatol. Sci.  2012  65:207-12  PubMed ID: 22169325
7976  Ogi T, Higa M, Maruyama S.  Melanin synthesis inhibitors from Balanophora fungosa.  J. Agric. Food Chem.  2011  59:1109-14  PubMed ID: 21265535
8083  Kim KS, Choi HW, Yoon HE, Kim IY.  Reactive oxygen species generated by NADPH oxidase 2 and 4 are required for chondrogenic differentiation.  J. Biol. Chem.  2010  285:40294-302  PubMed ID: 20952384
13479  Matsumoto Y, Iwakawa M, Furusawa Y, Ishikawa K, Aoki M, Imadome K, Matsumoto I, Tsujii H, Ando K, Imai T.  Gene expression analysis in human malignant melanoma cell lines exposed to carbon beams  Int J Radiat Biol  2008  84(4):299-314  PubMed ID: 18386195
3374  Yanagisawa, Ayano, Endo, Chisato, Okawa, Katsuya, Shitara, Shingo, Kugoh, Hiroyuki, Kakitani, Makoto, Oshimura, Mitsuo, Tomizuka, Kazuma  Generation of chromosome-specific monoclonal antibodies using in vitro-differentiated transchromosomic mouse embryonic stem cells.  Stem Cells  2005  23:1479-88  PubMed ID: 16081665
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014
12280  K Yasumoto , K Yokoyama, K Shibata, Y Tomita, S Shibahara  Microphthalmia-associated transcription factor as a regulator for melanocyte-specific transcription of the human tyrosinase gene  Mol Cell Biol  1995  15(3):1833   PubMed ID: 7862173
12279  K Yasumoto, K Yokoyama, K Shibata, Y Tomita, S Shibahara  Microphthalmia-associated transcription factor as a regulator for melanocyte-specific transcription of the human tyrosinase gene  Mol Cell Biol  1994  14(12):8058-70  PubMed ID: 7969144

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