細胞番号 : 細胞名
RCB0776 : F-36E
update : 2024/09/09
|
| 細胞特性(Comment:英) | Subline of F-36P. Erythropoietin-dependent |
| 細胞特性(日) | F-36Pの亜株でエリスロポエチン依存性の急性白血病細胞株。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | No restriction, but the user needs depositor's approval to use for any commercial purpose. |
| 使用条件(日) | 特に制限を設けない。ただし、商業目的で利用する場合は、寄託者の承諾を得ること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
|
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
|
細胞基本情報
|
|
寄託者 |
Chiba, Shigeru
|
| 樹立者 |
Chiba, Shigeru
|
| 寄託日 |
1992
|
| 動物種 |
_human
< Mammals
|
| 属名 |
Homo
|
| 種名 |
sapiens
|
| 人種 |
Japanese
|
| 性別 |
Male
|
| 採取組織 |
bone marrow
|
| 病名 |
myelodysplastic syndrome
|
| 細胞分類 |
cancer
|
| 初代培養日 |
1989
|
|
細胞寿命 |
infinite
|
| 細胞形態 |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_7227
|
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Suspension cells
|
| 培養方法 |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| 培地 |
|
RPMI1640 + 5% FBS + 5.2U/ml Erythropoietin
|
| 抗生物質 |
|
Free
|
|
継代方法 |
|
dilution
|
|
継代密度 |
|
1 : 8 split
|
| 継代・培地交換頻度 |
|
Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| 培養最適温度 |
|
37
℃
|
| 二酸化炭素濃度 |
|
5
%
|
| 凍結培地 |
|
Medium + 10% DMSO
|
| 凍結方法 |
|
Slow freezing
|
| マイコプラズマ/アコレプラズマ |
|
(-)
|
| ウィルス（HIV) |
|
(-)
|
| ウィルス（HTLV-1) |
|
(-)
|
| アイソザイム検査 |
|
LD, NP
|
| 染色体検査 |
|
42-86(50) : 42(5),43(25),44(9),45(2),46(4),81(1)/
|
| 個体識別検査 |
|
OK
|
| 画像情報 |
寄託時情報 | ロット情報 |
|
|
|
| 文献情報 |
Reference(英) |
1件
|
| Reference(日) |
1件
|
| 利用者成果(英) |
12件
|
| 利用者成果(日) |
0件
|
| 利用者成果(英) |
|
16835
Won Jeong Lee, Hookeun Oh, Inseong Choi, Keunho Lee, Hyunwoo Shin, Yoon Jung Lee, Jeehye Park, Yoo Hee Yang, Gyong-Sik Ha, Dong-Eok Lee, Kang-Yell Choi, Eunyoung Choi
Differences in Proportion of N-acetyllactosamine and O-acetylated Sialic Acid Have No Significant Effect on the Pharmacokinetics and Biological Activity of Darbepoetin Alfa
Biotechnology and Bioprocess Engineering
2022
27:244-252
DOI: 10.1007/s12257-021-0042-0
|
|
15014
Sebastian Samuel J, Kumar D, Chodisetti SB, Agrewala JN, Singh B, Guptasarma P, Sarkar D.
Probing protease sensitivity of recombinant human erythropoietin reveals α3-α4 inter-helical loop as a stability determinant
Proteins
2015
83(10):1813-22
PubMed ID: 26214268
DOI: 10.1002/prot.24865
|
|
18145
Kim TG, Cho YC, Chun BH, Park SH, Lee HS, Chung N.
Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells
Biotechnol Biotechnol Equip
2014
28(2):350-354
PubMed ID: 26019520
DOI: 10.1080/13102818.2014.901675
|
|
7671
Im SJ, Yang SI, Yang SH, Choi DH, Choi SY, Kim HS, Jang DS, Jin KS, Chung YK, Kim SH, Paik SH, Park YC, Chung MK, Kim YB, Han KH, Choi KY, Sung YC.
Natural form of noncytolytic flexible human Fc as a long-acting carrier of agonistic ligand, erythropoietin.
PLoS ONE
2011
6:e24574
PubMed ID: 21957455
DOI: 10.1371/journal.pone.0024574
|
|
18146
Eun Gyo Lee, Seung Hui Lee, Kyung Mi Park, Jung Eun Baek, Sun Hee Yeon, Jin-Ki Park, Won-Kyong Chang, Joon-Ki Jung, Bong Hyun Chung
Purification and characterization of recombinant human erythropoietin from milk of transgenic pigs
Journal of Chemical Technology & Biotechnology
2009
84:643-649
DOI: 10.1002/jctb.2094
|
|
8532
Joung CH, Shin JY, Koo JK, Lim JJ, Wang JS, Lee SJ, Tan HK, Kim SL, Lim SM.
Production and characterization of long-acting recombinant human albumin-EPO fusion protein expressed in CHO cell.
Protein Expr. Purif.
2009
68:137-45
PubMed ID: 19595770
DOI: 10.1016/j.pep.2009.07.003
|
|
8844
Yeon Sook Choi, Doo Young Lee, Ick Young Kim, Hong Jin Kim, Hong Woo Park, Tae Boo Choe and Ik-Hwan Kim
Enhancement of erythropoietin production in recombinant Chinese hamster ovary cells by sodium lactate addition
Biotechnology and Bioprocess Engineering
2007
12:60-72
DOI: 10.1007/BF02931805
|
|
8933
Lee DE, Son W, Ha BJ, Oh MS, Yoo OJ.
The prolonged half-lives of new erythropoietin derivatives via peptide addition.
Biochem. Biophys. Res. Commun.
2006
339:380-5
PubMed ID: 16314154
DOI: 10.1016/j.bbrc.2005.11.034
|
|
8951
Kang SH, Kim DM, Kim HJ, Jun SY, Lee KY, Kim HJ.
Cell-free production of aggregation-prone proteins in soluble and active forms.
Biotechnol. Prog.
2005
21:1412-9
PubMed ID: 16209544
DOI: 10.1021/bp050087y
|
|
9140
Akiko Ogawa, Satoshi Terada, Norio Sakuragawa, Seiji Masuda, Masaya Nagao, Masao Miki
Progesterone, but Not 17beta-estradiol, Up-Regulates Erythropoietin (EPO) Production in Human Amniotic Epithelial Cells
J Biosci Bioeng
2003
96 (5), 448-53
PubMed ID: 16233554
DOI: 10.1016/S1389-1723(03)70130-3
|
|
18144
Choi YS, Lee DY, Kim IY, Kang S, Ahn K, Kim HJ, Jeong YH, Chun GT, Park JK, Kim IH.
Ammonia removal using hepatoma cells in mammalian cell cultures
Biotechnol Prog
2000
16(5):760-8
PubMed ID: 11027167
DOI: 10.1021/bp000099d
|
|
1385
Chin H, Arai A, Wakao H, Kamiyama R, Miyasaka N, Miura O.
Lyn physically associates with the erythropoietin receptor and may play a role in activation of the Stat5 pathway
Blood
1998
91(10):3734-45
PubMed ID: 9573010
|
戻る
細胞材料の再培養方法 (
HPS以外・
HPS )
ページトップへ
細胞材料開発室サイト
