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Cell No. : Cell Name
RCB0776 : F-36E  update : 2023/02/20
CommentSubline of F-36P. Erythropoietin-dependent
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Chiba, Shigeru
Originator Chiba, Shigeru
Year of deposit 1992
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue bone marrow
Disease name myelodysplastic syndrome
Classification cancer
Year of origin 1989
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_7227
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 5% FBS + 5.2U/ml Erythropoietin
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 42-86(50) : 42(5),43(25),44(9),45(2),46(4),81(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 11

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1673  Chiba S, Takaku F, Tange T, Shibuya K, Misawa C, Sasaki K, Miyagawa K, Yazaki Y, Hirai H  Establishment and erythroid differentiation of a cytokine-dependent human leukemic cell line F-36: a parental line requiring granulocyte-macrophage colony-stimulating factor or interleukin-3, and a subline requiring erythropoietin.  Blood  1991  78:2261-8  PubMed ID: 1834251  

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User's Publication
16835  Won Jeong Lee, Hookeun Oh, Inseong Choi, Keunho Lee, Hyunwoo Shin, Yoon Jung Lee, Jeehye Park, Yoo Hee Yang, Gyong-Sik Ha, Dong-Eok Lee, Kang-Yell Choi, Eunyoung Choi  Differences in Proportion of N-acetyllactosamine and O-acetylated Sialic Acid Have No Significant Effect on the Pharmacokinetics and Biological Activity of Darbepoetin Alfa  Biotechnology and Bioprocess Engineering  2022  27:244-252    DOI: 10.1007/s12257-021-0042-0
15014  Sebastian Samuel J, Kumar D, Chodisetti SB, Agrewala JN, Singh B, Guptasarma P, Sarkar D.  Probing protease sensitivity of recombinant human erythropoietin reveals α3-α4 inter-helical loop as a stability determinant  Proteins  2015  83(10):1813-22  PubMed ID: 26214268   DOI: 10.1002/prot.24865
18145  Kim TG, Cho YC, Chun BH, Park SH, Lee HS, Chung N.  Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells  Biotechnol Biotechnol Equip  2014  28(2):350-354  PubMed ID: 26019520   DOI: 10.1080/13102818.2014.901675
7671  Im SJ, Yang SI, Yang SH, Choi DH, Choi SY, Kim HS, Jang DS, Jin KS, Chung YK, Kim SH, Paik SH, Park YC, Chung MK, Kim YB, Han KH, Choi KY, Sung YC.  Natural form of noncytolytic flexible human Fc as a long-acting carrier of agonistic ligand, erythropoietin.  PLoS ONE  2011  6:e24574  PubMed ID: 21957455   DOI: 10.1371/journal.pone.0024574
18146  Eun Gyo Lee, Seung Hui Lee, Kyung Mi Park, Jung Eun Baek, Sun Hee Yeon, Jin-Ki Park, Won-Kyong Chang, Joon-Ki Jung, Bong Hyun Chung  Purification and characterization of recombinant human erythropoietin from milk of transgenic pigs  Journal of Chemical Technology & Biotechnology  2009  84:643-649    DOI: 10.1002/jctb.2094
8532  Joung CH, Shin JY, Koo JK, Lim JJ, Wang JS, Lee SJ, Tan HK, Kim SL, Lim SM.  Production and characterization of long-acting recombinant human albumin-EPO fusion protein expressed in CHO cell.  Protein Expr. Purif.  2009  68:137-45  PubMed ID: 19595770   DOI: 10.1016/j.pep.2009.07.003
8844  Yeon Sook Choi, Doo Young Lee, Ick Young Kim, Hong Jin Kim, Hong Woo Park, Tae Boo Choe and Ik-Hwan Kim  Enhancement of erythropoietin production in recombinant Chinese hamster ovary cells by sodium lactate addition  Biotechnology and Bioprocess Engineering  2007  12:60-72    DOI: 10.1007/BF02931805
8933  Lee DE, Son W, Ha BJ, Oh MS, Yoo OJ.  The prolonged half-lives of new erythropoietin derivatives via peptide addition.  Biochem. Biophys. Res. Commun.  2006  339:380-5  PubMed ID: 16314154   DOI: 10.1016/j.bbrc.2005.11.034
8951  Kang SH, Kim DM, Kim HJ, Jun SY, Lee KY, Kim HJ.  Cell-free production of aggregation-prone proteins in soluble and active forms.  Biotechnol. Prog.  2005  21:1412-9  PubMed ID: 16209544   DOI: 10.1021/bp050087y
9140  Akiko Ogawa, Satoshi Terada, Norio Sakuragawa, Seiji Masuda, Masaya Nagao, Masao Miki  Progesterone, but Not 17beta-estradiol, Up-Regulates Erythropoietin (EPO) Production in Human Amniotic Epithelial Cells  J Biosci Bioeng  2003  96 (5), 448-53  PubMed ID: 16233554   DOI: 10.1016/S1389-1723(03)70130-3
18144  Choi YS, Lee DY, Kim IY, Kang S, Ahn K, Kim HJ, Jeong YH, Chun GT, Park JK, Kim IH.  Ammonia removal using hepatoma cells in mammalian cell cultures  Biotechnol Prog  2000  16(5):760-8  PubMed ID: 11027167   DOI: 10.1021/bp000099d

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