Cell No. : Cell Name
RCB0776 : F-36E
update : 2024/09/09
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| Comment | Subline of F-36P. Erythropoietin-dependent |
| Comment from the depositor | |
| Terms and conditions | No restriction, but the user needs depositor's approval to use for any commercial purpose. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Chiba, Shigeru
|
| Originator |
Chiba, Shigeru
|
| Year of deposit |
1992
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
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| Tissue |
bone marrow
|
| Disease name |
myelodysplastic syndrome
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| Classification |
cancer
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| Year of origin |
1989
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_7227
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 5% FBS + 5.2U/ml Erythropoietin
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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(-)
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| Virus (HTLV-1) |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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42-86(50) : 42(5),43(25),44(9),45(2),46(4),81(1)/
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
12
|
| User's Publication |
|
16835
Won Jeong Lee, Hookeun Oh, Inseong Choi, Keunho Lee, Hyunwoo Shin, Yoon Jung Lee, Jeehye Park, Yoo Hee Yang, Gyong-Sik Ha, Dong-Eok Lee, Kang-Yell Choi, Eunyoung Choi
Differences in Proportion of N-acetyllactosamine and O-acetylated Sialic Acid Have No Significant Effect on the Pharmacokinetics and Biological Activity of Darbepoetin Alfa
Biotechnology and Bioprocess Engineering
2022
27:244-252
DOI: 10.1007/s12257-021-0042-0
|
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15014
Sebastian Samuel J, Kumar D, Chodisetti SB, Agrewala JN, Singh B, Guptasarma P, Sarkar D.
Probing protease sensitivity of recombinant human erythropoietin reveals α3-α4 inter-helical loop as a stability determinant
Proteins
2015
83(10):1813-22
PubMed ID: 26214268
DOI: 10.1002/prot.24865
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18145
Kim TG, Cho YC, Chun BH, Park SH, Lee HS, Chung N.
Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells
Biotechnol Biotechnol Equip
2014
28(2):350-354
PubMed ID: 26019520
DOI: 10.1080/13102818.2014.901675
|
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7671
Im SJ, Yang SI, Yang SH, Choi DH, Choi SY, Kim HS, Jang DS, Jin KS, Chung YK, Kim SH, Paik SH, Park YC, Chung MK, Kim YB, Han KH, Choi KY, Sung YC.
Natural form of noncytolytic flexible human Fc as a long-acting carrier of agonistic ligand, erythropoietin.
PLoS ONE
2011
6:e24574
PubMed ID: 21957455
DOI: 10.1371/journal.pone.0024574
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18146
Eun Gyo Lee, Seung Hui Lee, Kyung Mi Park, Jung Eun Baek, Sun Hee Yeon, Jin-Ki Park, Won-Kyong Chang, Joon-Ki Jung, Bong Hyun Chung
Purification and characterization of recombinant human erythropoietin from milk of transgenic pigs
Journal of Chemical Technology & Biotechnology
2009
84:643-649
DOI: 10.1002/jctb.2094
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8532
Joung CH, Shin JY, Koo JK, Lim JJ, Wang JS, Lee SJ, Tan HK, Kim SL, Lim SM.
Production and characterization of long-acting recombinant human albumin-EPO fusion protein expressed in CHO cell.
Protein Expr. Purif.
2009
68:137-45
PubMed ID: 19595770
DOI: 10.1016/j.pep.2009.07.003
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8844
Yeon Sook Choi, Doo Young Lee, Ick Young Kim, Hong Jin Kim, Hong Woo Park, Tae Boo Choe and Ik-Hwan Kim
Enhancement of erythropoietin production in recombinant Chinese hamster ovary cells by sodium lactate addition
Biotechnology and Bioprocess Engineering
2007
12:60-72
DOI: 10.1007/BF02931805
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8933
Lee DE, Son W, Ha BJ, Oh MS, Yoo OJ.
The prolonged half-lives of new erythropoietin derivatives via peptide addition.
Biochem. Biophys. Res. Commun.
2006
339:380-5
PubMed ID: 16314154
DOI: 10.1016/j.bbrc.2005.11.034
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8951
Kang SH, Kim DM, Kim HJ, Jun SY, Lee KY, Kim HJ.
Cell-free production of aggregation-prone proteins in soluble and active forms.
Biotechnol. Prog.
2005
21:1412-9
PubMed ID: 16209544
DOI: 10.1021/bp050087y
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9140
Akiko Ogawa, Satoshi Terada, Norio Sakuragawa, Seiji Masuda, Masaya Nagao, Masao Miki
Progesterone, but Not 17beta-estradiol, Up-Regulates Erythropoietin (EPO) Production in Human Amniotic Epithelial Cells
J Biosci Bioeng
2003
96 (5), 448-53
PubMed ID: 16233554
DOI: 10.1016/S1389-1723(03)70130-3
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18144
Choi YS, Lee DY, Kim IY, Kang S, Ahn K, Kim HJ, Jeong YH, Chun GT, Park JK, Kim IH.
Ammonia removal using hepatoma cells in mammalian cell cultures
Biotechnol Prog
2000
16(5):760-8
PubMed ID: 11027167
DOI: 10.1021/bp000099d
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1385
Chin H, Arai A, Wakao H, Kamiyama R, Miyasaka N, Miura O.
Lyn physically associates with the erythropoietin receptor and may play a role in activation of the Stat5 pathway
Blood
1998
91(10):3734-45
PubMed ID: 9573010
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