Cell No. : Cell Name
RCB0775 : F-36P
update : 2018/07/06
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Comment | GM-CSF- or IL-3-dependent cell line. Differentiate to erythoid cells with erythropoietin. |
Comment from the depositor | |
Terms and conditions | No restriction, but the user needs depositor's approval to use for any commercial purpose. |
Remarks | |
Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Chiba, Shigeru
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Originator |
Chiba, Shigeru
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Year of deposit |
1992
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Animal |
human
< Mammals
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Genus |
Homo
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Species |
sapiens
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Race |
Japanese
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Gender |
Male
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Age at sampling |
65 years
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Tissue |
bone marrow
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Case history |
myelodysplastic syndrome
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Classification |
cancer
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Year of origin |
1989
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|
Lifespan |
infinite
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Morphology |
lymphocyte-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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Medium and additives |
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RPMI1640 + 5% FBS + 5ng/ml human IL-3
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Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 2-8 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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STR(human) |
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OK
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Virus (HIV) |
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(-)
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Virus (HTLV-1) |
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(-)
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Isozyme |
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LD, NP
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Chromosome mode |
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39-122(50) : /40(1),41(4),42(8),43(17),44(17),85(1)/
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Images |
deposit info | lot info |
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Reference information |
Reference |
2
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User's Publication |
1
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Reference |
4815
Honda H, Shibuya M, Chiba S, Yazaki Y, Hirai H.
Identification of novel protein-tyrosine phosphatases in a human leukemia cell line, F-36P.
Leukemia
1993
7(5):742-6
PubMed ID: 8483328
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1673
Chiba S, Takaku F, Tange T, Shibuya K, Misawa C, Sasaki K, Miyagawa K, Yazaki Y, Hirai H
Establishment and erythroid differentiation of a cytokine-dependent human leukemic cell line F-36: a parental line requiring granulocyte-macrophage colony-stimulating factor or interleukin-3, and a subline requiring erythropoietin.
Blood
1991
78:2261-8
PubMed ID: 1834251
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