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Cell No. : Cell Name
RCB0775 : F-36P  update : 2024/01/17
CommentGM-CSF- or IL-3-dependent cell line. Differentiate to erythoid cells with erythropoietin.
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Chiba, Shigeru
Originator Chiba, Shigeru
Year of deposit 1992
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 65 years
Tissue bone marrow
Disease name myelodysplastic syndrome
Classification cancer
Year of origin 1989
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2037
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 5% FBS + 5ng/ml human IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 39-122(50) : /40(1),41(4),42(8),43(17),44(17),85(1)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 15


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Reference
4815  Honda H, Shibuya M, Chiba S, Yazaki Y, Hirai H.  Identification of novel protein-tyrosine phosphatases in a human leukemia cell line, F-36P.  Leukemia  1993  7(5):742-6  PubMed ID: 8483328  
1673  Chiba S, Takaku F, Tange T, Shibuya K, Misawa C, Sasaki K, Miyagawa K, Yazaki Y, Hirai H  Establishment and erythroid differentiation of a cytokine-dependent human leukemic cell line F-36: a parental line requiring granulocyte-macrophage colony-stimulating factor or interleukin-3, and a subline requiring erythropoietin.  Blood  1991  78:2261-8  PubMed ID: 1834251  

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User's Publication
14629  Asayama T, Tamura H, Ishibashi M, Kuribayashi-Hamada Y, Onodera-Kondo A, Okuyama N, Yamada A, Shimizu M, Moriya K, Takahashi H, Inokuchi K.  Functional expression of Tim-3 on blasts and clinical impact of its ligand galectin-9 in myelodysplastic syndromes  Oncotarget  2017  8(51):88904-88917  PubMed ID: 29179486   DOI: 10.18632/oncotarget.21492
14398  Shintani T, Ohara-Waki F, Kitanaka A, Tanaka T, Kubota Y.  Cbl negatively regulates erythropoietin-induced growth and survival signaling through the proteasomal degradation of Src kinase  Blood Cells Mol Dis  2014  53(4):211-8  PubMed ID: 25084697   DOI: 10.1016/j.bcmd.2014.06.005
17142  Hu XM, Yuan B, Tanaka S, Song MM, Onda K, Tohyama K, Zhou AX, Toyoda H, Hirano T.  Arsenic disulfide-triggered apoptosis and erythroid differentiation in myelodysplastic syndrome and acute myeloid leukemia cell lines  Hematology  2014  19(6):352-60  PubMed ID: 24192507   DOI: 10.1179/1607845413Y.0000000138
15792  Kon A, Shih LY, Minamino M, Sanada M, Shiraishi Y, Nagata Y, Yoshida K, Okuno Y, Bando M, Nakato R, Ishikawa S, Sato-Otsubo A, Nagae G, Nishimoto A, Haferlach C, Nowak D, Sato Y, Alpermann T, Nagasaki M, Shimamura T, Tanaka H, Chiba K, Yamamoto R, Yamaguchi T, Otsu M, Obara N, Sakata-Yanagimoto M, Nakamaki T, Ishiyama K, Nolte F, Hofmann WK, Miyawaki S, Chiba S, Mori H, Nakauchi H, Koeffler HP, Aburatani H, Haferlach T, Shirahige K, Miyano S, Ogawa S.  Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms  Nat Genet  2013  45(10):1232-7  PubMed ID: 23955599   DOI: 10.1038/ng.2731
8517  Fukumoto T, Kubota Y, Kitanaka A, Yamaoka G, Ohara-Waki F, Imataki O, Ohnishi H, Ishida T, Tanaka T.  Gab1 transduces PI3K-mediated erythropoietin signals to the Erk pathway and regulates erythropoietin-dependent proliferation and survival of erythroid cells.  Cell. Signal.  2009  21:1775-83  PubMed ID: 19665053   DOI: 10.1016/j.cellsig.2009.07.013
13527  Satoh C, Tamura H, Yamashita T, Tsuji T, Dan K, Ogata K.  Aggressive characteristics of myeloblasts expressing CD7 in myelodysplastic syndromes  Leuk Res  2009  33(2):326-31  PubMed ID: 18718658   DOI: 10.1016/j.leukres.2008.07.006
18143  Nogami W, Yoshida H, Koizumi K, Yamada H, Abe K, Arimura A, Yamane N, Takahashi K, Yamane A, Oda A, Tanaka Y, Takemoto H, Ohnishi Y, Ikeda Y, Miyakawa Y.  The effect of a novel, small non-peptidyl molecule butyzamide on human thrombopoietin receptor and megakaryopoiesis  Haematologica  2008  93(10):1495-504  PubMed ID: 18728031   DOI: 10.3324/haematol.12752
12487  Manabe N, Kubota Y, Kitanaka A, Ohnishi H, Taminato T, Tanaka T.  Src transduces signaling via growth hormone (GH)-activated GH receptor (GHR) tyrosine-phosphorylating GHR and STAT5 in human leukemia cells  Leuk Res  2006  30(11):1391-8  PubMed ID: 16650892   DOI: 10.1016/j.leukres.2006.03.024
17308  Kobayashi N, Saeki K, Yuo A.  Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce cell cycle progression through the synthesis of c-Myc protein by internal ribosome entry site-mediated translation via phosphatidylinositol 3-kinase pathway in human factor-dependent leukemic cells  Blood  2003  102(9):3186-95  PubMed ID: 12855588   DOI: 10.1182/blood-2003-02-0567
12276  Tauro S, Hepburn MD, Peddie CM, Bowen DT, Pippard MJ.  Functional disturbance of marrow stromal microenvironment in the myelodysplastic syndromes  Leukemia  2002  16(5):785-90  PubMed ID: 11986938   DOI: 10.1038/sj.leu.2402440
18102  Kawasaki A, Matsumura I, Miyagawa Ji, Ezoe S, Tanaka H, Terada Y, Tatsuka M, Machii T, Miyazaki H, Furukawa Y, Kanakura Y.  Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells  J Cell Biol  2001  152(2):275-87  PubMed ID: 11266445   DOI: 10.1083/jcb.152.2.275
18142  Kubota Y, Tanaka T, Kitanaka A, Ohnishi H, Okutani Y, Waki M, Ishida T, Kamano H.  Src transduces erythropoietin-induced differentiation signals through phosphatidylinositol 3-kinase  EMBO J  2001  20(20):5666-77  PubMed ID: 11598010   DOI: 10.1093/emboj/20.20.5666
4877  Miyoshi S, Motohashi T, Nakamura Y, Osawa M, Hiroyama T, Kim DK, Tokumoto Y, Nakauchi H.  A transmembrane trap method for efficient cloning of genes encoding proteins possessing transmembrane domain.  Biochem Biophys Res Commun  2001  289(5):1192-8  PubMed ID: 11741319   DOI: 10.1006/bbrc.2001.6097
1394  Matsumura I, Nakajima K, Wakao H, Hattori S, Hashimoto K, Sugahara H, Kato T, Miyazaki H, Hirano T, Kanakura Y.  Involvement of prolonged ras activation in thrombopoietin-induced megakaryocytic differentiation of a human factor-dependent hematopoietic cell line  Mol Cell Biol  1998  18(7):4282-90  PubMed ID: 9632812   DOI: 10.1128/MCB.18.7.4282
1411  Okajima Y, Matsumura I, Nishiura T, Hashimoto K, Yoshida H, Ishikawa J, Wakao H, Yoshimura A, Kanakura Y, Tomiyama Y, Matsuzawa Y.  Insulin-like growth factor-I augments erythropoietin-induced proliferation through enhanced tyrosine phosphorylation of STAT5  J Biol Chem  1998  273(36):22877-83  PubMed ID: 9722506   DOI: 10.1074/jbc.273.36.22877



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