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Cell No. : Cell Name
RCB0775 : F-36P  update : 2018/07/06
CommentGM-CSF- or IL-3-dependent cell line. Differentiate to erythoid cells with erythropoietin.
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Chiba, Shigeru
Originator Chiba, Shigeru
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 65 years
Tissue bone marrow
Case history myelodysplastic syndrome
Classification cancer
Year of origin 1989
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 5% FBS + 5ng/ml human IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 39-122(50) : /40(1),41(4),42(8),43(17),44(17),85(1)/
deposit info
lot info
Reference information Reference 2
User's Publication 1

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4815  Honda H, Shibuya M, Chiba S, Yazaki Y, Hirai H.  Identification of novel protein-tyrosine phosphatases in a human leukemia cell line, F-36P.  Leukemia  1993  7(5):742-6  PubMed ID: 8483328
1673  Chiba S, Takaku F, Tange T, Shibuya K, Misawa C, Sasaki K, Miyagawa K, Yazaki Y, Hirai H  Establishment and erythroid differentiation of a cytokine-dependent human leukemic cell line F-36: a parental line requiring granulocyte-macrophage colony-stimulating factor or interleukin-3, and a subline requiring erythropoietin.  Blood  1991  78:2261-8  PubMed ID: 1834251

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User's Publication
8517  Fukumoto T, Kubota Y, Kitanaka A, Yamaoka G, Ohara-Waki F, Imataki O, Ohnishi H, Ishida T, Tanaka T.  Gab1 transduces PI3K-mediated erythropoietin signals to the Erk pathway and regulates erythropoietin-dependent proliferation and survival of erythroid cells.  Cell. Signal.  2009  21:1775-83  PubMed ID: 19665053

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