Cell No. : Cell Name
RCB0775 : F-36P
update : 2018/07/06
|
| Comment | GM-CSF- or IL-3-dependent cell line. Differentiate to erythoid cells with erythropoietin. |
| Comment from the depositor | |
| Terms and conditions | No restriction, but the user needs depositor's approval to use for any commercial purpose. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
|
Depositor |
Chiba, Shigeru
|
| Originator |
Chiba, Shigeru
|
| Year of deposit |
1992
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
65 years
|
| Tissue |
bone marrow
|
| Case history |
myelodysplastic syndrome
|
| Classification |
cancer
|
| Year of origin |
1989
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Medium and additives |
|
RPMI1640 + 5% FBS + 5ng/ml human IL-3
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| Antibiotics |
|
Free
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|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 2-8 split
|
| SC frequency |
|
Subculture : 2 times/week
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| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Virus (HIV) |
|
(-)
|
| Virus (HTLV-1) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
39-122(50) : /40(1),41(4),42(8),43(17),44(17),85(1)/
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
1
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| Reference |
|
4815
Honda H, Shibuya M, Chiba S, Yazaki Y, Hirai H.
Identification of novel protein-tyrosine phosphatases in a human leukemia cell line, F-36P.
Leukemia
1993
7(5):742-6
PubMed ID: 8483328
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1673
Chiba S, Takaku F, Tange T, Shibuya K, Misawa C, Sasaki K, Miyagawa K, Yazaki Y, Hirai H
Establishment and erythroid differentiation of a cytokine-dependent human leukemic cell line F-36: a parental line requiring granulocyte-macrophage colony-stimulating factor or interleukin-3, and a subline requiring erythropoietin.
Blood
1991
78:2261-8
PubMed ID: 1834251
|
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