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Cell No. : Cell Name
RCB0775 : F-36P  update : 2022/11/16
CommentGM-CSF- or IL-3-dependent cell line. Differentiate to erythoid cells with erythropoietin.
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Chiba, Shigeru
Originator Chiba, Shigeru
Year of deposit 1992
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 65 years
Tissue bone marrow
Disease name myelodysplastic syndrome
Classification cancer
Year of origin 1989
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2037
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 5% FBS + 5ng/ml human IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 39-122(50) : /40(1),41(4),42(8),43(17),44(17),85(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 7

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4815  Honda H, Shibuya M, Chiba S, Yazaki Y, Hirai H.  Identification of novel protein-tyrosine phosphatases in a human leukemia cell line, F-36P.  Leukemia  1993  7(5):742-6  PubMed ID: 8483328  
1673  Chiba S, Takaku F, Tange T, Shibuya K, Misawa C, Sasaki K, Miyagawa K, Yazaki Y, Hirai H  Establishment and erythroid differentiation of a cytokine-dependent human leukemic cell line F-36: a parental line requiring granulocyte-macrophage colony-stimulating factor or interleukin-3, and a subline requiring erythropoietin.  Blood  1991  78:2261-8  PubMed ID: 1834251  

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User's Publication
14629  Asayama T, Tamura H, Ishibashi M, Kuribayashi-Hamada Y, Onodera-Kondo A, Okuyama N, Yamada A, Shimizu M, Moriya K, Takahashi H, Inokuchi K.  Functional expression of Tim-3 on blasts and clinical impact of its ligand galectin-9 in myelodysplastic syndromes  Oncotarget  2017  8(51):88904-88917  PubMed ID: 29179486   DOI: 10.18632/oncotarget.21492
14398  Shintani T, Ohara-Waki F, Kitanaka A, Tanaka T, Kubota Y.  Cbl negatively regulates erythropoietin-induced growth and survival signaling through the proteasomal degradation of Src kinase  Blood Cells Mol Dis  2014  53(4):211-8  PubMed ID: 25084697   DOI: 10.1016/j.bcmd.2014.06.005
17142  Hu XM, Yuan B, Tanaka S, Song MM, Onda K, Tohyama K, Zhou AX, Toyoda H, Hirano T.  Arsenic disulfide-triggered apoptosis and erythroid differentiation in myelodysplastic syndrome and acute myeloid leukemia cell lines  Hematology  2014  19(6):352-60  PubMed ID: 24192507   DOI: 10.1179/1607845413Y.0000000138
15792  Kon A, Shih LY, Minamino M, Sanada M, Shiraishi Y, Nagata Y, Yoshida K, Okuno Y, Bando M, Nakato R, Ishikawa S, Sato-Otsubo A, Nagae G, Nishimoto A, Haferlach C, Nowak D, Sato Y, Alpermann T, Nagasaki M, Shimamura T, Tanaka H, Chiba K, Yamamoto R, Yamaguchi T, Otsu M, Obara N, Sakata-Yanagimoto M, Nakamaki T, Ishiyama K, Nolte F, Hofmann WK, Miyawaki S, Chiba S, Mori H, Nakauchi H, Koeffler HP, Aburatani H, Haferlach T, Shirahige K, Miyano S, Ogawa S.  Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms  Nat Genet  2013  45(10):1232-7  PubMed ID: 23955599   DOI: 10.1038/ng.2731
8517  Fukumoto T, Kubota Y, Kitanaka A, Yamaoka G, Ohara-Waki F, Imataki O, Ohnishi H, Ishida T, Tanaka T.  Gab1 transduces PI3K-mediated erythropoietin signals to the Erk pathway and regulates erythropoietin-dependent proliferation and survival of erythroid cells.  Cell. Signal.  2009  21:1775-83  PubMed ID: 19665053   DOI: 10.1016/j.cellsig.2009.07.013
17308  Kobayashi N, Saeki K, Yuo A.  Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce cell cycle progression through the synthesis of c-Myc protein by internal ribosome entry site-mediated translation via phosphatidylinositol 3-kinase pathway in human factor-dependent leukemic cells  Blood  2003  102(9):3186-95  PubMed ID: 12855588   DOI: 10.1182/blood-2003-02-0567
4877  Miyoshi S, Motohashi T, Nakamura Y, Osawa M, Hiroyama T, Kim DK, Tokumoto Y, Nakauchi H.  A transmembrane trap method for efficient cloning of genes encoding proteins possessing transmembrane domain.  Biochem Biophys Res Commun  2001  289(5):1192-8  PubMed ID: 11741319   DOI: 10.1006/bbrc.2001.6097

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