細胞番号 : 細胞名 RCB0774 : EC-GI-10  update : 2024/02/13
細胞特性(Comment:英)			Produce hypercalcemia in nude mice. PTH-related protein, IL-1alpha producing.
細胞特性(日)			高カルシウム血症患者食道癌由来の扁平上皮癌。ヌードマウス移植で高カルシウム血症を惹起。PTHrP、IL-1 alpha産生性という。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Sato, Kanji
		樹立者	Sato, Kanji
		寄託日	1992
		別名	EC-GI
		動物種	_human < Mammals
		属名	Homo
		種名	sapiens
		人種	Japanese
		性別	Male
		齢	65 years
		採取組織	esophagus, lymph node meta
		病名	Esophageal carcinoma
		転移	Yes
		転移巣	lympho node
		細胞分類	cancer
		細胞寿命	infinite
		細胞形態	epithelial-like
		Cellosaurus(Expasy)	CVCL_1187
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		HamF12 + 10% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 4 split
		継代・培地交換頻度		Subculture : once/week, Medium Renewal : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ		(-)
		ウィルス（HIV)		(-)
		アイソザイム検査		LD, NP
		染色体検査		62-134(50) : /67(6),68(5),69(5),70(4),71(1),72(6)/
		個体識別検査		OK
画像情報			寄託時情報	ロット情報
文献情報		Reference(英)	5件
		Reference(日)	1件
		利用者成果(英)	21件
		利用者成果(日)	0件

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Reference(英)

1581  Sato K, Satoh T, Shizume K, Yamakawa Y, Ono Y, Demura H, Akatsu T, Takahashi N, Suda T  Prolonged decrease of serum calcium concentration by murine gamma-interferon in hypercalcemic, human tumor (EC-GI)-bearing nude mice.  Cancer Res  1992  52:444-9  PubMed ID: 1728416

1997  Yasuda T, Banville D, Hendy, G N, Goltzman D  Characterization of the human parathyroid hormone-like peptide gene. Functional and evolutionary aspects.  J Biol Chem  1989  264:7720-5  PubMed ID: 2708388

124  Sato K, Fujii Y, Kasono K, Ozawa M, Imamura H, Kanaji Y, Kurosawa H, Tsushima T, Shizume K.  Parathyroid hormone-related protein and interleukin-1 alpha synergistically stimulate bone resorption in vitro and increase the serum calcium concentration in mice in vivo  Endocrinology  1989  124(5):2172-8  PubMed ID: 2539970   DOI: 10.1210/endo-124-5-2172

1580  Sato K, Fujii Y, Kasono K, Tsushima T, Shizume K  Production of interleukin-1 alpha and a parathyroid hormone-like factor by a squamous cell carcinoma of the esophagus (EC-GI) derived from a patient with hypercalcemia.  J Clin Endocrinol Metab  1988  67:592-601  PubMed ID: 3261732   DOI: 10.1210/jcem-67-3-592

1579  Sato K, Kasono K, Ohba Y, Yashiro T, Fujii Y, Yoshida, M A, Tsushima T, Shizume K  Establishment of A Parathyroid Hormone-like Factor-producing Esophageal Carcinoma Cell Line (EC-GI)  Jpn J Cancer Res  1987  78:1044-1048  PubMed ID: 2824416

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Reference(日)

1690  佐藤幹二  ヒト食道ガン細胞 : EC-GI  生体の科学  1992  43:371

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利用者成果(英)

21175  Zhang X, Wang Y, Zhang X, Shen Y, Yang K, Ma Q, Qiao Y, Shi J, Wang Y, Xu L, Yang B, Ge G, Hu L, Kong X, Yang C, Chen Y, Ding J, Meng L.  Intact regulation of G1/S transition renders esophageal squamous cell carcinoma sensitive to PI3Kα inhibitors.  Signal Transduct Target Ther  2023  8(1):153  PubMed ID: 37041169   DOI: 10.1038/s41392-023-01359-x

11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826   DOI: 10.1038/s41586-019-1103-9

11101  Sato N, Fujishima F, Nakamura Y, Aoyama Y, Onodera Y, Ozawa Y, Ito K, Ishida H, Kamei T, Watanabe M, Sasano H.  Myosin 5a regulates tumor migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma: utility as a prognostic factor.  Hum. Pathol.  2018  80:113-122  PubMed ID: 29898384   DOI: 10.1016/j.humpath.2018.06.002

12638  Nakayama A, Aoki S, Uchihashi K, Nishijima-Matsunobu A, Yamamoto M, Kakihara N, Iwakiri R, Fujimoto K, Toda S.  Interaction between Esophageal Squamous Cell Carcinoma and Adipose Tissue in Vitro  Am J Pathol  2016  186(5):1180-94  PubMed ID: 26952643   DOI: 10.1016/j.ajpath.2016.01.003

12875  Ueno N, Shimizu A, Kanai M, Iwaya Y, Ueda S, Nakayama J, Seo MK.  Enhanced Expression of Fibroblast Growth Factor Receptor 3 IIIc Promotes Human Esophageal Carcinoma Cell Proliferation  J Histochem Cytochem  2016  64(1):7-17  PubMed ID: 26487184   DOI: 10.1369/0022155415616161

7382  Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor AK, Yasuda Y, Niki T.  The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.  BMC Cancer  2015  15:82  PubMed ID: 25884729   DOI: 10.1186/s12885-015-1065-8

15741  Zuguchi M, Miki Y, Onodera Y, Fujishima F, Takeyama D, Okamoto H, Miyata G, Sato A, Satomi S, Sasano H.  Estrogen receptor α and β in esophageal squamous cell carcinoma  Cancer Sci  2012  103(7):1348-55  PubMed ID: 22463081   DOI: 10.1111/j.1349-7006.2012.02288.x

6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

3369  Shimada, Hideaki, Nakashima, Kazue, Ochiai, Takenori, Nabeya, Yoshihiro, Takiguchi, Masaki, Nomura, Fumio, Hiwasa, Takaki  Serological identification of tumor antigens of esophageal squamous cell carcinoma.  Int J Oncol  2005  26:77-86  PubMed ID: 15586227

18636  Nakashima K, Shimada H, Ochiai T, Kuboshima M, Kuroiwa N, Okazumi S, Matsubara H, Nomura F, Takiguchi M, Hiwasa T.  Serological identification of TROP2 by recombinant cDNA expression cloning using sera of patients with esophageal squamous cell carcinoma  Int J Cancer  2004  112(6):1029-35  PubMed ID: 15386348   DOI: 10.1002/ijc.20517

12151  Nakajima Y, Miyake S, Tanaka K, Ogiya K, Toukairin Y, Kawada K, Nishikage T, Nagai K, Kawano T.  The expressions of p21 and pRB may be good indicators for the sensitivity of esophageal squamous cell cancers to CPT-11: Cell proliferation activity correlates with the effect of CPT-11  Cancer Sci  2004  95(5):464-8  PubMed ID: 15132777   DOI: 10.1111/j.1349-7006.2004.tb03233.x

4700  Ikeguchi M, Yamaguchi K, Kaibara N.  Survivin gene expression positively correlates with proliferative activity of cancer cells in esophageal cancer  Tumour Biol  2003  24(1):40-5  PubMed ID: 12743425   DOI: 10.1159/000070659

3796  Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.  Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.  Int J Cancer  2003  107(1):53-9  PubMed ID: 12925956   DOI: 10.1002/ijc.11348

13721  Ikeguchi M, Fukuda K, Yamaguchi K, Kondo A, Tsujitani S, Kaibara N.  Quantitative analysis of heparanase gene expression in esophageal squamous cell carcinoma  Ann Surg Oncol  2003  10(3):297-304  PubMed ID: 12679316   DOI: 10.1245/aso.2003.05.022

13750  Ito M, Amizuka N, Tanaka S, Funatsu-Ozawa Y, Kenmotsu S, Oda K, Nakajima T, Ozawa H.  Ultrastructural and cytobiological studies on possible interactions between PTHrP-secreting tumor cells, stromal cells, and bone cells  J Bone Miner Metab  2003  21(6):353-62  PubMed ID: 14586791   DOI: 10.1007/s00774-003-0429-3

16678  Okamoto Y, Ozaki T, Miyazaki K, Aoyama M, Miyazaki M, Nakagawara A.  UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme  Cancer Res  2003  63(14):4167-73  PubMed ID: 12874022

4070  Sugiura T, Nakane S, Kishimoto S, Waku K, Yoshioka Y, Tokumura A.  Lysophosphatidic acid, a growth factor-like lipid, in the saliva  J Lipid Res  2002  43(12):2049-55  PubMed ID: 12454265   DOI: 10.1194/jlr.m200242-jlr200

5948  Ikeguchi M, Kaibara N.  survivin messenger RNA expression is a good prognostic biomarker for oesophageal carcinoma.  Br J Cancer  2002  87(8):883-7  PubMed ID: 12373603   DOI: 10.1038/sj.bjc.6600546

18756  Shimada H, Shimizu T, Ochiai T, Liu TL, Sashiyama H, Nakamura A, Matsubara H, Gunji Y, Kobayashi S, Tagawa M, Sakiyama S, Hiwasa T.  Preclinical study of adenoviral p53 gene therapy for esophageal cancer  Surg Today  2001  31(7):597-604  PubMed ID: 11495154   DOI: 10.1007/s005950170093

12275  Nakajima Y, Miyake S, Nagai K, Kawano T, Iwai T.  CPT-11 may provide therapeutic efficacy for esophageal squamous cell cancer and the effects correlate with the level of DNA topoisomerase I protein  Jpn J Cancer Res  2001  92(12):1335-41  PubMed ID: 11749700   DOI: 10.1111/j.1349-7006.2001.tb02158.x

13674  Miyauchi M, Shimada H, Kadomatsu K, Muramatsu T, Matsubara S, Ikematsu S, Takenaga K, Asano T, Ochiai T, Sakiyama S, Tagawa M.  Frequent expression of midkine gene in esophageal cancer suggests a potential usage of its promoter for suicide gene therapy  Jpn J Cancer Res  1999  90(4):469-75  PubMed ID: 10363587   DOI: 10.1111/j.1349-7006.1999.tb00771.x

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利用者成果(日)