細胞番号 : 細胞名
RCB0774 : EC-GI-10
update : 2023/01/27
|
| 細胞特性(Comment:英) | Produce hypercalcemia in nude mice. PTH-related protein, IL-1alpha producing. |
| 細胞特性(日) | 高カルシウム血症患者食道癌由来の扁平上皮癌。ヌードマウス移植で高カルシウム血症を惹起。PTHrP、IL-1 alpha産生性という。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
|
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
|
細胞基本情報
|
|
寄託者 |
Sato, Kanji
|
| 樹立者 |
Sato, Kanji
|
| 寄託日 |
1992
|
| 別名 |
EC-GI
|
| 動物種 |
human
< Mammals
|
| 属名 |
Homo
|
| 種名 |
sapiens
|
| 人種 |
Japanese
|
| 性別 |
Male
|
| 齢 |
65 years
|
| 採取組織 |
esophagus, lymph node meta
|
| 病名 |
Esophageal carcinoma
|
| 転移 |
Yes
|
| 転移巣 |
lympho node
|
| 細胞分類 |
cancer
|
|
細胞寿命 |
infinite
|
| 細胞形態 |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_1187
|
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Adherent cells
|
| 培地 |
|
HamF12 + 10% FBS
|
| 抗生物質 |
|
Free
|
|
継代方法 |
|
0.25% Trypsin
|
|
継代密度 |
|
1 : 4 split
|
| 継代・培地交換頻度 |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| 培養最適温度 |
|
37
℃
|
| 二酸化炭素濃度 |
|
5
%
|
| 凍結培地 |
|
Medium + 10% DMSO
|
| 凍結方法 |
|
Slow freezing
|
| マイコプラズマ |
|
(-)
|
| ウィルス（HIV) |
|
(-)
|
| アイソザイム検査 |
|
LD, NP
|
| 染色体検査 |
|
62-134(50) : /67(6),68(5),69(5),70(4),71(1),72(6)/
|
| 個体識別検査 |
|
OK
|
| 画像情報 |
寄託時情報 | ロット情報 |
|
|
|
| 文献情報 |
Reference(英) |
5件
|
| Reference(日) |
1件
|
| 利用者成果(英) |
15件
|
| 利用者成果(日) |
0件
|
| Reference(英) |
|
1581
Sato K, Satoh T, Shizume K, Yamakawa Y, Ono Y, Demura H, Akatsu T, Takahashi N, Suda T
Prolonged decrease of serum calcium concentration by murine gamma-interferon in hypercalcemic, human tumor (EC-GI)-bearing nude mice.
Cancer Res
1992
52:444-9
PubMed ID: 1728416
|
|
1997
Yasuda T, Banville D, Hendy, G N, Goltzman D
Characterization of the human parathyroid hormone-like peptide gene. Functional and evolutionary aspects.
J Biol Chem
1989
264:7720-5
PubMed ID: 2708388
|
|
124
Sato K, Fujii Y, Kasono K, Ozawa M, Imamura H, Kanaji Y, Kurosawa H, Tsushima T, Shizume K.
Parathyroid hormone-related protein and interleukin-1 alpha synergistically stimulate bone resorption in vitro and increase the serum calcium concentration in mice in vivo
Endocrinology
1989
124(5):2172-8
PubMed ID: 2539970
DOI: 10.1210/endo-124-5-2172
|
|
1580
Sato K, Fujii Y, Kasono K, Tsushima T, Shizume K
Production of interleukin-1 alpha and a parathyroid hormone-like factor by a squamous cell carcinoma of the esophagus (EC-GI) derived from a patient with hypercalcemia.
J Clin Endocrinol Metab
1988
67:592-601
PubMed ID: 3261732
DOI: 10.1210/jcem-67-3-592
|
|
1579
Sato K, Kasono K, Ohba Y, Yashiro T, Fujii Y, Yoshida, M A, Tsushima T, Shizume K
Establishment of A Parathyroid Hormone-like Factor-producing Esophageal Carcinoma Cell Line (EC-GI)
Jpn J Cancer Res
1987
78:1044-1048
PubMed ID: 2824416
|
| 利用者成果(英) |
|
21175
Zhang X, Wang Y, Zhang X, Shen Y, Yang K, Ma Q, Qiao Y, Shi J, Wang Y, Xu L, Yang B, Ge G, Hu L, Kong X, Yang C, Chen Y, Ding J, Meng L.
Intact regulation of G1/S transition renders esophageal squamous cell carcinoma sensitive to PI3Kα inhibitors.
Signal Transduct Target Ther
2023
8(1):153
PubMed ID: 37041169
DOI: 10.1038/s41392-023-01359-x
|
|
11511
Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature
2019
PubMed ID: 30971826
DOI: 10.1038/s41586-019-1103-9
|
|
11101
Sato N, Fujishima F, Nakamura Y, Aoyama Y, Onodera Y, Ozawa Y, Ito K, Ishida H, Kamei T, Watanabe M, Sasano H.
Myosin 5a regulates tumor migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma: utility as a prognostic factor.
Hum. Pathol.
2018
80:113-122
PubMed ID: 29898384
DOI: 10.1016/j.humpath.2018.06.002
|
|
12638
Nakayama A, Aoki S, Uchihashi K, Nishijima-Matsunobu A, Yamamoto M, Kakihara N, Iwakiri R, Fujimoto K, Toda S.
Interaction between Esophageal Squamous Cell Carcinoma and Adipose Tissue in Vitro
Am J Pathol
2016
186(5):1180-94
PubMed ID: 26952643
DOI: 10.1016/j.ajpath.2016.01.003
|
|
12875
Ueno N, Shimizu A, Kanai M, Iwaya Y, Ueda S, Nakayama J, Seo MK.
Enhanced Expression of Fibroblast Growth Factor Receptor 3 IIIc Promotes Human Esophageal Carcinoma Cell Proliferation
J Histochem Cytochem
2016
64(1):7-17
PubMed ID: 26487184
DOI: 10.1369/0022155415616161
|
|
7382
Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor AK, Yasuda Y, Niki T.
The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.
BMC Cancer
2015
15:82
PubMed ID: 25884729
DOI: 10.1186/s12885-015-1065-8
|
|
15741
Zuguchi M, Miki Y, Onodera Y, Fujishima F, Takeyama D, Okamoto H, Miyata G, Sato A, Satomi S, Sasano H.
Estrogen receptor α and β in esophageal squamous cell carcinoma
Cancer Sci
2012
103(7):1348-55
PubMed ID: 22463081
DOI: 10.1111/j.1349-7006.2012.02288.x
|
|
6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
|
|
3369
Shimada, Hideaki, Nakashima, Kazue, Ochiai, Takenori, Nabeya, Yoshihiro, Takiguchi, Masaki, Nomura, Fumio, Hiwasa, Takaki
Serological identification of tumor antigens of esophageal squamous cell carcinoma.
Int J Oncol
2005
26:77-86
PubMed ID: 15586227
|
|
3796
Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.
Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.
Int J Cancer
2003
107(1):53-9
PubMed ID: 12925956
DOI: 10.1002/ijc.11348
|
|
13721
Ikeguchi M, Fukuda K, Yamaguchi K, Kondo A, Tsujitani S, Kaibara N.
Quantitative analysis of heparanase gene expression in esophageal squamous cell carcinoma
Ann Surg Oncol
2003
10(3):297-304
PubMed ID: 12679316
DOI: 10.1245/aso.2003.05.022
|
|
13750
Ito M, Amizuka N, Tanaka S, Funatsu-Ozawa Y, Kenmotsu S, Oda K, Nakajima T, Ozawa H.
Ultrastructural and cytobiological studies on possible interactions between PTHrP-secreting tumor cells, stromal cells, and bone cells
J Bone Miner Metab
2003
21(6):353-62
PubMed ID: 14586791
DOI: 10.1007/s00774-003-0429-3
|
|
16678
Okamoto Y, Ozaki T, Miyazaki K, Aoyama M, Miyazaki M, Nakagawara A.
UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme
Cancer Res
2003
63(14):4167-73
PubMed ID: 12874022
|
|
5948
Ikeguchi M, Kaibara N.
survivin messenger RNA expression is a good prognostic biomarker for oesophageal carcinoma.
Br J Cancer
2002
87(8):883-7
PubMed ID: 12373603
DOI: 10.1038/sj.bjc.6600546
|
|
13674
Miyauchi M, Shimada H, Kadomatsu K, Muramatsu T, Matsubara S, Ikematsu S, Takenaga K, Asano T, Ochiai T, Sakiyama S, Tagawa M.
Frequent expression of midkine gene in esophageal cancer suggests a potential usage of its promoter for suicide gene therapy
Jpn J Cancer Res
1999
90(4):469-75
PubMed ID: 10363587
DOI: 10.1111/j.1349-7006.1999.tb00771.x
|
戻る
細胞材料の再培養方法 (
HPS以外・
HPS )
ページトップへ
細胞材料開発室サイト
