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Cell No. : Cell Name
RCB0772 : A6  update : 2023/06/30
CommentFrog (Xenopus laevis) cell line derived from kidney.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Laboratory of Tissue Morphogenesis
Biological Sciences, Faculty of Science and Engineering
Chuo University
Dr.FUKUI Akimasa
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Asashima, Makoto
Originator Rafferty, K. A. Jr.
Year of deposit 1992
Animal _frog < Amphibians
Genus Xenopus
Species laevis
Gender Male
Tissue kidney
Classification other
Year of origin 1965
History Dr. Sekiya, Kunio (Niigata Univ.) -> Dr. Asashima, M.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4101
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium (L-15 : D.D.W. = 1 : 1) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
deposit info
lot info
Reference information Reference 6
User's Publication 0

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841  Masayuki Ikuzawa, Saori Akiduki, Makoto Asashima   Gene expression profile of Xenopus A6 cells cultured under random positioning machine shows downregulation of ion transporter genes and inhibition of dome formation  Advances in Space Research  2007  40:1694-1702    DOI: 10.1016/j.asr.2007.08.014
839  Tanaka M, Asashima M, Atomi Y.  Proliferation and differentiation of Xenopus A6 cells under hypergravity as revealed by time-lapse imaging  In Vitro Cell Dev Biol Anim  2003  39(1-2):71-9  PubMed ID: 12892530   DOI: 10.1290/1543-706X(2003)039<0071:PADOXA>2.0.CO;2
840  Kyuno J, Fukui A, Michiue T, Asashima M.  Characterization of a gene respondent to clinorotation in Xenopus A6 cells  Biol Sci Space  2003  17(3):171-2  PubMed ID: 14676358  
854  Ichigi J, Asashima M.  Dome formation and tubule morphogenesis by Xenopus kidney A6 cell cultures exposed to microgravity simulated with a 3D-clinostat and to hypergravity  In Vitro Cell Dev Biol Anim  2001  37(1):31-44  PubMed ID: 11249203   DOI: 10.1290/1071-2690(2001)037<0031:dfatmb>;2
838  Nakaya M, Fukui A, Izumi Y, Akimoto K, Asashima M, Ohno S.  Meiotic maturation induces animal-vegetal asymmetric distribution of aPKC and ASIP/PAR-3 in Xenopus oocytes  Development . 2000 Dec;127(23):5021-31  2000  127(23):5021-31  PubMed ID: 11060229   DOI: 10.1242/dev.127.23.5021
2213  Rafferty, K. A.  Mass Culture of Amphibian Cells : Methods and Observations Concerning Stability of Cell Type  Biology of Amphibian tumors  1969  52-81   

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