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Cell No. : Cell Name
RCB0771 : XTC-YF  update : 2024/06/18
CommentFrog (Xenopus laevis) cell line derived from tadpole.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Laboratory of Tissue Morphogenesis
Biological Sciences, Faculty of Science and Engineering
Chuo University
Dr.FUKUI Akimasa
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Asashima, Makoto
Originator Pudney, Mary
Year of deposit 1992
Another name XTC-2, XTC
Animal _frog < Amphibians
Genus Xenopus
Species laevis
Gender Unknown
Tissue tadpole
Classification other
Year of origin 1969
History Dr. Grunz, H. (Essen, Germany) -> Dr. Asashima, M.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5610
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium (L-15 : D.D.W. = 1 : 1) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
deposit info
lot info
Reference information Reference 3
User's Publication 6

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123  Ariizumi T, Asashima M.  Control of the embryonic body plan by activin during amphibian development  Zoolog Sci  1995  12(5):509-21  PubMed ID: 8590829   DOI: 10.2108/zsj.12.509
121  Fukui A, Nakamura T, Sugino K, Takio K, Uchiyama H, Asashima M, Sugino H.  Isolation and characterization of Xenopus follistatin and activins  Dev Biol  1993  159(1):131-9  PubMed ID: 8365557  
2212  Pudney M, Varma, M G, Leake, C J  Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.  Experientia  1973  29:466-7  PubMed ID: 4708349   DOI: 10.1007/bf01926785

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User's Publication
16030  Suzuki S, Sasaki K, Fukazawa T, Kubo T.  Xenopus laevis il11ra.L is an experimentally proven interleukin-11 receptor component that is required for tadpole tail regeneration  Sci Rep  2022  12(1):1903  PubMed ID: 35115663   DOI: 10.1038/s41598-022-05954-w
15880  Wurtz N, Penant G, Jardot P, Duclos N, La Scola B.  Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile  Eur J Clin Microbiol Infect Dis  2021  40(3):477-484  PubMed ID: 33389257   DOI: 10.1007/s10096-020-04106-0
4814  Sugita S, Munechika R, Nakamura M.  Multinucleation of Incubated Cells and Their Morphological Differences Compared to Mononuclear Cells.  Micromachines (Basel)  2019  10(2). pii: E156.  PubMed ID: 30823567   DOI: 10.3390/mi10020156
10562  Kazuaki Nagayama, Yuya Suzuki, Daisuke Fujiwara  Directional Dependence of Cyclic Stretch-induced Cell Migration in Wound Healing Process of Monolayer Cells  Advanced Biomedical Engineering  2019  8:163-169    DOI: 10.14326/abe.8.163
7666  Fuchigami T, Matsuzaki T, Ihara S.  Possible roles of ENaC and Cl(-) channel in wound closure in Xenopus laevis embryos.  Zool. Sci.  2011  28:703-11  PubMed ID: 21967216   DOI: 10.2108/zsj.28.703
15720  Sugita S, Adachi T, Ueki Y, Sato M.  A novel method for measuring tension generated in stress fibers by applying external forces  Biophys J  2011  101(1):53-60  PubMed ID: 21723814   DOI: 10.1016/j.bpj.2011.05.046

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