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Cell No. : Cell Name
RCB0771 : XTC-YF  update : 2018/07/06
CommentFrog (Xenopus laevis) cell line derived from tadpole.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
National Institute of Advanced Industrial Science and Technology
Biotechnology Research Institute for Drug Discovery
1-1-1 Higashi, Central 4, Tsukuba, Ibaraki, 305-8562 Japan
Dr.Asashima, Makoto
Fax. +81-29-861-3273
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Asashima, Makoto
Originator Pudney, Mary
Year of deposit 1992
Another name XTC-2, XTC
Animal frog < Amphibians
Genus Xenopus
Species laevis
Gender Unknown
Tissue tadpole
Classification other
Year of origin 1969
History Dr. Grunz, H. (Essen, Germany) -> Dr. Asashima, M.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives (L-15 : D.D.W. = 1 : 1) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
deposit info
lot info
Reference information Reference 1
User's Publication 3

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2212  Pudney M, Varma, M G, Leake, C J  Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.  Experientia  1973  29:466-7  PubMed ID: 4708349

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User's Publication
4814  Sugita S, Munechika R, Nakamura M.  Multinucleation of Incubated Cells and Their Morphological Differences Compared to Mononuclear Cells.  Micromachines (Basel)  2019  10(2). pii: E156.  PubMed ID: 30823567
10562  Kazuaki Nagayama, Yuya Suzuki, Daisuke Fujiwara  Directional Dependence of Cyclic Stretch-induced Cell Migration in Wound Healing Process of Monolayer Cells  Advanced Biomedical Engineering  2019  8:163-169 
7666  Fuchigami T, Matsuzaki T, Ihara S.  Possible roles of ENaC and Cl(-) channel in wound closure in Xenopus laevis embryos.  Zool. Sci.  2011  28:703-11  PubMed ID: 21967216

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