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Cell No. : Cell Name
RCB0770 : XTY update : 2023/10/20 |
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| Comment | Frog (Xenopus laevis) cell line derived from subcutaneous tumor. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) A permission of the approver is required. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Experientia 1992;48:87-91) designated by the DEPOSITOR is required. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Asashima, Makoto | ||
| Originator | Fukui, Akimasa | |||
| Year of deposit | 1992 | |||
| Animal | _frog < Amphibians | |||
| Genus | Xenopus | |||
| Species | laevis | |||
| Gender | Female | |||
| Tissue | subcutaneous | |||
| Disease name | subcutaneous tumoe on the back | |||
| Classification | cancer | |||
| Year of origin | 1983 | Lifespan | infinite | |
| Morphology | other | |||
| Cellosaurus(Expasy) | CVCL_5611 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | (L-15 : D.D.W. = 1 : 1) + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 4 split |
| SC frequency | Subculture : once/2-3 weeks, Medium Renewal : 2 times/week | |||
| Temperature | 25 ℃ | |||
| CO2 concentration | 0 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
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| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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| Reference |
| 2211 Fukui A, Tashiro A, Koyama H, Iwamura Y, Asashima M A new cell line (XTY) from a tumor of Xenopus laevis Experientia 1992 48:87-91 PubMed ID: 1737584 DOI: 10.1007/bf01923615 |
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