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Cell No. : Cell Name
RCB0769 : HP100-1  update : 2022/03/23
CommentHydrogen peroxide resistant HL60-derived clone. More resistant than HP50-2.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamada, Michiyuki
Originator Kasugai, Isao
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue blood
Disease name promyelocytic leukemia
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_8710
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS (heat inactivated)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 42-46(50) : 42(5),43(34),44(10),46(1)
STR(human) OK
Reference information Reference 3
User's Publication 0


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Reference
1539  Kasugai I, Yamada M  High production of catalase in hydrogen peroxide-resistant human leukemia HL-60 cell lines.  Leuk Res  1992  16:173-9  PubMed ID: 1312186  
1540  Yamada M, Hashinaka K, Inazawa J, Abe T  Expression of catalase and myeloperoxidase genes in hydrogen peroxide-resistant HL-60 cells.  DNA Cell Biol  1991  10:735-42  PubMed ID: 1660277  
1538  Kasugai I, Yamada M  Adaptation of human leukemia HL-60 cells to hydrogen peroxide as oxidative stress.  Leuk Res  1989  13:757-62  PubMed ID: 2796382  

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