Cell No. : Cell Name
RCB0768 : HP50-2
update : 2024/08/30
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| Comment | Hydrogen peroxide resistant HL60-derived clone. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Yamada, Michiyuki
|
| Originator |
Kasugai, Isao
|
| Year of deposit |
1992
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
blood
|
| Disease name |
promyelocytic leukemia
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_8711
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS (heat inactivated)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Virus (HTLV-1) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
39-90(50) : /39(1),43(2),44(28),45(11),46(3),74(1)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
1
|
| Reference |
|
2293
Emi IIMURA, Tadao OHNO
EXAMINATION OF 254 PRIMATE CELL LINES BY A PCR PROTOCOL USEFUL FOR ROUTINE DETECTION OF LATENT HIV-I DNA
TISSUE CULTURE RESEARCH COMMUNICATIONS
1998
17:81-86
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|
1539
Kasugai I, Yamada M
High production of catalase in hydrogen peroxide-resistant human leukemia HL-60 cell lines.
Leuk Res
1992
16:173-9
PubMed ID: 1312186
DOI: 10.1016/0145-2126(92)90129-u
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1540
Yamada M, Hashinaka K, Inazawa J, Abe T
Expression of catalase and myeloperoxidase genes in hydrogen peroxide-resistant HL-60 cells.
DNA Cell Biol
1991
10:735-42
PubMed ID: 1660277
DOI: 10.1089/dna.1991.10.735
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1538
Kasugai I, Yamada M
Adaptation of human leukemia HL-60 cells to hydrogen peroxide as oxidative stress.
Leuk Res
1989
13:757-62
PubMed ID: 2796382
DOI: 10.1016/0145-2126(89)90088-x
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