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Cell No. : Cell Name
RCB0764 : Fvp^(r)B350  update : 2022/03/23
CommentTopoisomerase II inhibitor VP-16 resistant FM3A mutant.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kusano, Toshihisa
Originator Kusano, Toshihisa
Year of deposit 1992
Another name 50B3
Animal mouse < Mammals
Strain name C3H/HeN
Tissue breast
Classification cancer
Year of origin 1966
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U119
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives ES + 5% CS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0

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1672  Hong JH, Okada K, Kusano T, Komazawa Y, Kobayashi M, Mizutani A, Kamada N, Kuramoto A.  Reduced DNA topoisomerase II in VP-16 resistant mouse breast cancer cell line.  Biomed Pharmacother  1990  44(1):41-5  PubMed ID: 1369691  

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