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Cell No. : Cell Name
RCB0763 : GI-1  update : 2021/02/26
CommentHuman glioma separated from gliosarcoma.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nakatu, Syouji
Originator Iwasaki, Kouji
Year of deposit 1992
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 61 years
Tissue brain
Disease name gliosarcoma
Metastastatic ability No
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 81-194(50) : /93(4),94(4),95(3),96(4),97(4),98(6)/
deposit info
lot info
Reference information Reference 4
User's Publication 8

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1587  Iwasaki K, Kikuchi H, Miyatake S, Kondo S, Oda Y  Establishment of a new cell line derived from a human gliosarcoma.  Neurosurgery  1992  30:228-35  PubMed ID: 1545891
4812  Miyatake S, Kondou S, Aoki T, Iwasaki K, Ohyama K, Ootsuka S, Oda Y, Kikuchi H.  Immunomodulatory effects of interferons on target human gliosarcoma cells in the tumor-specific CTL- and LAK-mediated cytolysis]  No Shinkei Geka  1991  19(11):1053-9  PubMed ID: 1762655
4811  Miyatake S, Kikuchi H, Oda Y, Nishioka T, Takahashi J, Kondoh S, Matsumoto M, Yamasaki T, Iwasaki K, Aoki T, et al.  Decreased susceptibility of lined human gliosarcoma cells to lymphokine-activated killer cell cytolysis by gamma-interferon treatment.  Cancer Res  1990  50(3):596-600  PubMed ID: 2137025
4810  Miyatake S, Kikuchi H, Iwasaki K, Yamashita J, Li ZY, Namba Y, Hanaoka M.  Specific cytotoxic activity of T lymphocyte clones derived from a patient with gliosarcoma.  J Neurosurg  1988  69(5):751-9  PubMed ID: 3263476

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User's Publication
11920  Takumi Chinen , Shohei Yamamoto, Yutaka Takeda , Koki Watanabe, Kanako Kuroki , Kaho Hashimoto , Daisuke Takao , Daiju Kitagawa  NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells  EMBO J  2020    PubMed ID: 31782546
11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826
12337  Agarwal S, Mohamed MS, Mizuki T, Maekawa T, Sakthi Kumar D.  Chlorotoxin modified morusin-PLGA nanoparticles for targeted glioblastoma therapy  J Mater Chem B  2019  7(39):5896-5919  PubMed ID: 31423502
9629  Sakai S, Maruyama H, Kimura T, Tajiri K, Honda J, Homma S, Aonuma K, Miyauchi T.  Antagonists to endothelin receptor type B promote apoptosis in human pulmonary arterial smooth muscle cells.  Life Sci.  2016  159:116-120  PubMed ID: 27021787
2958  Awata T, Kurihara S, Takata N, Neda T, Iizuka H, Ohkubo T, Osaki M, Watanabe M, Nakashima Y, Inukai K, Inoue I, Kawasaki I, Mori K, Yoneya S, Katayama S  Functional VEGF C-634G polymorphism is associated with development of diabetic macular edema and correlated with macular retinal thickness in type 2 diabetes.  Biochem Biophys Res Commun  2005  333:679-85  PubMed ID: 15963467
2959  Lambrechts, Diether, Storkebaum, Erik, Morimoto, Masafumi, Del-Favero, Jurgen, Desmet, Frederik, Marklund, Stefan L, Wyns, Sabine, Thijs, Vincent, Andersson, Jorgen, van Marion, Ingrid, Al-Chalabi, Ammar, Bornes, Stephanie, Musson, Rhiannon, Hansen, Valerie, Beckman, Lars, Adolfsson, Rolf, Pall, Hardev Singh, Prats, Herve, Vermeire, Severine, Rutgeerts, Paul, Katayama, Shigehiro, Awata, Takuya, Leigh, Nigel, Lang-Lazdunski, Loic, Dewerchin, Mieke, Shaw, Christopher, Moons, Lieve, Vlietinck, Robert, Morrison, Karen E, Robberecht, Wim, Van Broeckhoven, Christine, Collen, Desire, Andersen, Peter M, Carmeliet, Peter  VEGF is a modifier of amyotrophic lateral sclerosis in mice and humans and protects motoneurons against ischemic death.  Nat Genet  2003  34:383-94  PubMed ID: 12847526
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959
4988  Kuramoto T.  Detection of MAGE-1 tumor antigen in brain tumor.  Kurume Med J  1997  44(1):43-51  PubMed ID: 9154761

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